##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632331.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	963119
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78915066570175	34.0	31.0	34.0	31.0	34.0
2	32.9523807546108	34.0	31.0	34.0	31.0	34.0
3	33.03814066589902	34.0	33.0	34.0	31.0	34.0
4	36.42665859566679	37.0	37.0	37.0	35.0	37.0
5	36.294939669968095	37.0	37.0	37.0	35.0	37.0
6	36.323968273910076	37.0	37.0	37.0	35.0	37.0
7	36.302832775596784	37.0	37.0	37.0	35.0	37.0
8	36.29393356376522	37.0	37.0	37.0	35.0	37.0
9	38.08591150210929	39.0	39.0	39.0	37.0	39.0
10	38.05155230039071	39.0	38.0	39.0	35.0	39.0
11	38.12164955732366	39.0	38.0	39.0	37.0	39.0
12	38.08876473208399	39.0	38.0	39.0	37.0	39.0
13	38.10740728819596	39.0	38.0	39.0	37.0	39.0
14	39.47984309311726	40.0	39.0	41.0	37.0	41.0
15	39.48836851936261	40.0	39.0	41.0	37.0	41.0
16	39.49284667834401	40.0	39.0	41.0	37.0	41.0
17	39.49102343531796	40.0	39.0	41.0	37.0	41.0
18	39.48842874037372	40.0	39.0	41.0	37.0	41.0
19	39.503387431874984	41.0	39.0	41.0	37.0	41.0
20	39.48338159666666	41.0	39.0	41.0	37.0	41.0
21	39.44451620204772	40.0	39.0	41.0	37.0	41.0
22	39.407731547191986	40.0	39.0	41.0	37.0	41.0
23	39.35338000807792	40.0	39.0	41.0	36.0	41.0
24	39.29927765935466	40.0	39.0	41.0	36.0	41.0
25	39.25044464910359	40.0	39.0	41.0	36.0	41.0
26	39.17554320909462	40.0	39.0	41.0	36.0	41.0
27	39.092444443521515	40.0	39.0	41.0	36.0	41.0
28	39.01739037439818	40.0	39.0	41.0	35.0	41.0
29	38.948689621947025	40.0	39.0	41.0	35.0	41.0
30	38.87879275562002	40.0	38.0	41.0	35.0	41.0
31	38.80718685852943	40.0	38.0	41.0	35.0	41.0
32	38.720119735982784	40.0	38.0	41.0	35.0	41.0
33	38.63272970422139	40.0	38.0	41.0	35.0	41.0
34	38.56092549311144	40.0	38.0	41.0	35.0	41.0
35	38.52334135241855	40.0	38.0	41.0	35.0	41.0
36	38.48234953313142	40.0	38.0	41.0	35.0	41.0
37	38.421012356728504	40.0	38.0	41.0	34.0	41.0
38	38.379536692765896	40.0	38.0	41.0	34.0	41.0
39	38.330046442859086	40.0	38.0	41.0	34.0	41.0
40	38.24597168158867	40.0	38.0	41.0	34.0	41.0
41	38.19507973573359	40.0	37.0	41.0	34.0	41.0
42	38.11969652763573	40.0	37.0	41.0	34.0	41.0
43	37.25446388244858	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	0.0
13	1.0
14	2.0
15	1.0
16	7.0
17	6.0
18	15.0
19	20.0
20	69.0
21	114.0
22	208.0
23	373.0
24	631.0
25	923.0
26	1400.0
27	1949.0
28	2970.0
29	4256.0
30	6118.0
31	8176.0
32	11320.0
33	15703.0
34	23329.0
35	35456.0
36	57345.0
37	105699.0
38	243928.0
39	443098.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.165278641580116	17.843070274805086	11.90527857928252	27.086372504332278
2	20.154414978834392	19.750415057744682	32.86572064303581	27.229449320385125
3	20.61427507919582	20.499128352778836	28.901413013345184	29.985183554680162
4	15.491024473611256	14.72185680066534	32.42153877142908	37.36557995429433
5	17.174305563486964	33.83268318868177	31.726089922429107	17.26692132540216
6	36.736789534834216	32.797400944223924	14.799209651143835	15.66659986979802
7	31.544388595801763	28.67215785380623	19.537461102937435	20.245992447454572
8	28.660009822254572	31.066358362777603	19.255252985352797	21.01837882961503
9	27.88471621886807	13.252152641573886	18.875860615354902	39.98727052420313
10	18.449745047081407	25.151512949074828	30.889744673295823	25.50899733054794
11	38.592946458329656	20.006458184294985	19.85580182718854	21.54479353018682
12	24.327627219481705	24.12900171214564	26.415115889106122	25.128255179266528
13	32.44573100520289	17.69054498976762	23.080013996193614	26.783710008835875
14	24.478906552565157	19.574112856251407	23.038793752381583	32.90818683880185
15	27.48798435084346	25.928052504415344	20.366642128335126	26.21732101640607
16	27.176496362339442	24.133674031973204	22.109521253344603	26.58030835234275
17	26.117437201425787	24.201370754808078	23.147399231039987	26.533792812726155
18	26.253453623072538	22.541866581388177	24.591042228426602	26.61363756711268
19	26.45353274102162	23.460548488816023	24.14789865011489	25.938020120047472
20	27.142232683604	22.773509815505662	23.931206839445593	26.153050661444745
21	27.90153657024729	23.092785003722284	23.54319663509909	25.462481790931342
22	27.998720822660545	22.9078649678804	22.853250740562693	26.240163468896366
23	26.60668100203609	22.8230364056778	23.525753307742864	27.044529284543238
24	26.306095093129716	23.442586014812292	23.511840177589686	26.739478714468305
25	26.468068847151805	22.66303540891624	23.614735043125513	27.25416070080644
26	26.689329148319157	23.37416248667091	23.522638427857824	26.413869937152107
27	26.295919818838588	23.050318807956234	23.846793594561007	26.806967778644175
28	25.957747692652728	22.810784544796643	24.254116054194757	26.977351708355872
29	25.397796118652003	23.47819947483125	24.49167756009382	26.63232684642292
30	25.11278460917083	23.95259568132287	24.60505918790928	26.329560521597017
31	26.76377477757162	23.59210024929422	23.02051979038935	26.623605182744814
32	25.27735409643045	23.245933264736756	23.61078952860446	27.86592311022833
33	25.27787324307796	22.79874034257449	24.680543110456757	27.242843303890794
34	26.17059781813047	22.258412511849524	24.8311994675632	26.73979020245681
35	25.74271715125545	22.763646029203038	25.449087807425663	26.04454901211584
36	25.241948295070493	22.559725226062408	25.26873626208184	26.92959021678526
37	25.027229241661725	22.056256807310415	25.806260700910272	27.110253250117587
38	24.496453709250883	21.29965248323416	26.925021726287195	27.27887208122776
39	24.477556771281638	21.16706242946095	27.37740611492453	26.977974684332878
40	23.912829048123857	21.790765211775494	27.829686674232363	26.46671906586829
41	23.79145256193679	21.171423261300003	28.68171015212035	26.355414024642855
42	23.43168393521465	21.65786367001378	28.817311256449102	26.093141138322473
43	21.91619104181311	20.987437689423633	29.272187548994467	27.82418371976879
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	25.0
2	24.0
3	96.0
4	168.0
5	168.0
6	203.5
7	239.0
8	227.0
9	215.0
10	391.0
11	567.0
12	567.0
13	993.0
14	1419.0
15	2264.0
16	3109.0
17	3043.5
18	2978.0
19	2978.0
20	3328.0
21	3678.0
22	3496.0
23	3314.0
24	4025.5
25	4737.0
26	4737.0
27	5957.0
28	7177.0
29	8899.5
30	10622.0
31	12767.0
32	14912.0
33	14912.0
34	18372.0
35	21832.0
36	26265.0
37	30698.0
38	34325.0
39	37952.0
40	37952.0
41	41381.0
42	44810.0
43	49066.5
44	53323.0
45	58139.5
46	62956.0
47	62956.0
48	65739.5
49	68523.0
50	70582.0
51	72641.0
52	74605.5
53	76570.0
54	76570.0
55	76063.0
56	75556.0
57	76320.0
58	77084.0
59	75212.5
60	73341.0
61	73341.0
62	66352.5
63	59364.0
64	53871.5
65	48379.0
66	42844.0
67	37309.0
68	37309.0
69	31145.5
70	24982.0
71	21458.0
72	17934.0
73	13811.0
74	9688.0
75	9688.0
76	7708.5
77	5729.0
78	4805.5
79	3882.0
80	3415.5
81	2949.0
82	2949.0
83	2355.5
84	1762.0
85	1515.5
86	1269.0
87	1030.5
88	792.0
89	792.0
90	634.5
91	477.0
92	279.0
93	81.0
94	60.0
95	39.0
96	39.0
97	24.5
98	10.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	963119.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.86023834277614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.17136895446114	51.309275533256596
2	6.630912162455678	7.805941406243087
3	2.1222836043872992	3.7475435635560737
4	1.04817398281614	2.467830818130198
5	0.6449776762609638	1.8981769875245122
6	0.41157993323720515	1.4535415780467476
7	0.30883588866621664	1.2724707810987612
8	0.22764885266675144	1.0719572581139616
9	0.17085467933580817	0.9050892432915834
>10	1.0607923013237004	12.05738820720897
>50	0.11348684571731094	4.714737963295222
>100	0.08554999496607275	9.495129632428787
>500	0.002474586594056994	0.9598648468245594
>1k	0.0010605371117387117	0.8410521809808952
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1668	0.17318732160823325	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1509	0.15667845821752038	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1428	0.14826828252791194	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1254	0.13020197919467896	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	1169	0.12137648618706515	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	1056	0.10964377195341386	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.0382932950133888E-4	0.0
11	0.0	0.0	0.0	2.0765865900267775E-4	0.0
12	0.0	0.0	0.0	3.1148798850401665E-4	0.0
13	0.0	0.0	0.0	3.1148798850401665E-4	0.0
14	0.0	0.0	0.0	4.153173180053555E-4	0.0
15	0.0	0.0	0.0	7.268053065093721E-4	0.0
16	0.0	0.0	0.0	0.001661269272021422	0.0
17	0.0	0.0	0.0	0.002699562567034811	0.0
18	0.0	0.0	0.0	0.0028033918965361497	0.0
19	0.0	0.0	0.0	0.0030110505555388275	0.0
20	0.0	0.0	0.0	0.0036340265325468606	0.0
21	0.0	0.0	0.0	0.00467231982756025	0.0
22	0.0	0.0	0.0	0.006748906417587027	0.0
23	0.0	0.0	0.0	0.009759956973125854	0.0
24	0.0	0.0	0.0	0.012251860881157988	0.0
25	0.0	0.0	0.0	0.01380930082367807	0.0
26	0.0	0.0	0.0	0.01941608461675037	0.0
27	0.0	0.0	0.0	0.04236236643654626	0.0
28	0.0	0.0	0.0	0.12771007528664682	0.0
29	0.0	1.0382932950133888E-4	0.0	0.25562780923229633	0.0
30	0.0	1.0382932950133888E-4	0.0	0.4190551738674037	0.0
31	0.0	1.0382932950133888E-4	0.0	0.9272997417764576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	25	0.0054959077	29.599998	4
GGTATCA	670	0.0	26.783583	1
TCAATCG	45	0.003825364	20.555557	13
CTTATAC	890	0.0	20.16292	37
CTAGACA	270	0.0	19.185184	4
TCTAGAC	255	0.0	18.862745	3
GTAGACC	90	2.1514788E-6	18.5	4
CTCGTAT	50	0.007034076	18.499998	29
GTATCAA	1030	0.0	17.422329	2
CTCTAGT	255	0.0	17.411764	27
GTTCTAG	280	0.0	17.178572	1
GAACGGT	65	0.0015796826	17.076923	20
GTCTAGA	210	0.0	16.738094	1
TCTTATA	1615	0.0	16.724459	37
TAGCGCC	90	4.4447515E-5	16.444445	29
AAGACGG	255	0.0	15.960784	5
TATACAC	290	0.0	15.948276	37
ATTCCGC	70	0.0025920845	15.857143	28
CCTCTAG	410	0.0	15.792682	26
CGCAAGA	260	0.0	15.653846	2
>>END_MODULE