FastQCFastQC Report
Fri 10 Feb 2017
ERR1632329.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632329.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006946
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31420.31203262141167454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22850.22692378737290778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22600.22444103258764622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.026.0924841
GTCTATA450.003825483420.5555571
ATTACGT555.1424844E-420.18181817
ATACACT1052.2586391E-819.380954
ATAGAGT500.00703429218.4999984
TTTCGAG953.605488E-617.52631612
TGATAGA852.722573E-517.4117642
CTAGACT650.001579750317.0769234
CTTATAC9300.016.90860237
AAGACCG904.4450753E-516.4444455
TTATAGT904.4450753E-516.4444454
TACGACG803.3820453E-416.18755
AGGTCGC2650.016.05660410
CGGTTTA1059.340716E-615.85714215
TAACGCC1059.340716E-615.8571424
ATAACGC700.002592194415.8571423
GTAAGGT700.002592194415.8571424
CCTATAA700.002592194415.8571421
TACACTG2800.015.8571425
GTATCAA14150.015.8197882
Produced by FastQC (version 0.11.5)