##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632329.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1006946
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83385504287221	34.0	31.0	34.0	31.0	34.0
2	32.996057385401	34.0	33.0	34.0	31.0	34.0
3	33.08450602117691	34.0	33.0	34.0	31.0	34.0
4	36.46306157430488	37.0	37.0	37.0	35.0	37.0
5	36.341217900463384	37.0	37.0	37.0	35.0	37.0
6	36.365479380224954	37.0	37.0	37.0	35.0	37.0
7	36.34279196699724	37.0	37.0	37.0	35.0	37.0
8	36.33241504509676	37.0	37.0	37.0	35.0	37.0
9	38.139087895478006	39.0	39.0	39.0	37.0	39.0
10	38.11233372991203	39.0	38.0	39.0	37.0	39.0
11	38.17776822193047	39.0	39.0	39.0	37.0	39.0
12	38.15223457861693	39.0	38.0	39.0	37.0	39.0
13	38.16111290972902	39.0	39.0	39.0	37.0	39.0
14	39.581856425270075	41.0	39.0	41.0	37.0	41.0
15	39.593464793544044	41.0	39.0	41.0	37.0	41.0
16	39.58464505544488	41.0	39.0	41.0	37.0	41.0
17	39.57880065068037	41.0	39.0	41.0	37.0	41.0
18	39.57233257791381	41.0	39.0	41.0	37.0	41.0
19	39.60847254966999	41.0	39.0	41.0	37.0	41.0
20	39.57928528441446	41.0	39.0	41.0	37.0	41.0
21	39.55531279730989	41.0	39.0	41.0	37.0	41.0
22	39.51967632822416	41.0	39.0	41.0	37.0	41.0
23	39.47155756118004	41.0	39.0	41.0	37.0	41.0
24	39.432140353107314	41.0	39.0	41.0	37.0	41.0
25	39.38115946634676	41.0	39.0	41.0	37.0	41.0
26	39.30104096942637	40.0	39.0	41.0	36.0	41.0
27	39.22390773685977	40.0	39.0	41.0	36.0	41.0
28	39.165809288680826	40.0	39.0	41.0	36.0	41.0
29	39.108949238588764	40.0	39.0	41.0	36.0	41.0
30	39.03387272008628	40.0	39.0	41.0	35.0	41.0
31	38.96156000421075	40.0	38.0	41.0	35.0	41.0
32	38.87564775072347	40.0	38.0	41.0	35.0	41.0
33	38.79542696430593	40.0	38.0	41.0	35.0	41.0
34	38.717920325419634	40.0	38.0	41.0	35.0	41.0
35	38.69121680805127	40.0	38.0	41.0	35.0	41.0
36	38.6508174221855	40.0	38.0	41.0	35.0	41.0
37	38.60168767739283	40.0	38.0	41.0	35.0	41.0
38	38.54817835315896	40.0	38.0	41.0	35.0	41.0
39	38.493741471737316	40.0	38.0	41.0	35.0	41.0
40	38.42455206138164	40.0	38.0	41.0	35.0	41.0
41	38.3711082818741	40.0	38.0	41.0	34.0	41.0
42	38.31487090668219	40.0	38.0	41.0	34.0	41.0
43	37.47337990319242	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	1.0
14	2.0
15	0.0
16	1.0
17	3.0
18	20.0
19	32.0
20	59.0
21	116.0
22	197.0
23	348.0
24	587.0
25	852.0
26	1273.0
27	1844.0
28	2669.0
29	3922.0
30	5648.0
31	7755.0
32	10573.0
33	14736.0
34	22261.0
35	33816.0
36	54384.0
37	103737.0
38	246432.0
39	495675.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.94957227100559	17.973555682231222	12.39967187912758	26.6772001676356
2	18.179822949790754	20.259179737543025	34.6681947194785	26.89280259318772
3	19.316924641440554	21.571663227223702	30.44413503802587	28.667277093309867
4	14.72988621038268	15.34084250793985	34.196272689895984	35.73299859178148
5	15.405294822165242	35.39256325562642	33.66466523527578	15.537476686932566
6	34.722318773797205	34.56203212486072	15.755661177461352	14.959987923880725
7	29.988698500217488	30.29765250569544	20.602495069248995	19.111153924838074
8	27.908944471699577	33.07635166136019	19.926192665743745	19.08851120119649
9	27.07046852562104	14.151900896373787	19.09536360440381	39.682266973601365
10	17.174009331185587	26.511848698937186	32.59062551517162	23.723516454705614
11	36.32677422622464	21.187034855890982	21.32229533659203	21.163895581292344
12	22.28709384614468	24.80619616146248	28.66161641239947	24.245093579993366
13	30.768680743555265	18.735860711497935	24.710858377708437	25.784600167238363
14	23.29121919149587	19.81129077428184	24.78246102571538	32.11502900850691
15	25.74249264608033	26.616323020300992	22.00008739296844	25.641096940650243
16	25.71269958865719	25.607033544996455	23.39817626764494	25.28209059870142
17	24.469733232963833	25.77953534747643	25.01206618825637	24.738665231303365
18	24.233871528363984	24.643228137357912	26.169327848762496	24.95357248551561
19	25.313174688612893	24.648888818268308	26.16019131115273	23.877745181966066
20	25.652418302471037	24.2074550174488	25.710216833871925	24.429909846208236
21	25.591839085710653	24.77570793269947	24.70509838660663	24.927354594983246
22	25.771888462737824	24.457716699803168	24.744226602022355	25.026168235436657
23	25.101643980908612	24.660905351428973	25.09330192483013	25.14414874283229
24	25.294305752244906	24.47350702023743	25.19628659332278	25.03590063419488
25	25.334427069574733	23.904956174412533	25.31635261473803	25.444264141274708
26	24.848005752046287	24.70718390062625	25.290035414014255	25.154774933313206
27	25.18526316207622	24.49485871139068	24.995183455716592	25.324694670816513
28	24.631410224580065	24.74114798608863	25.50533990899214	25.122101880339166
29	24.451460157744307	24.993495182462613	25.62798799538406	24.927056664409015
30	24.30944658402735	25.68995755482419	25.30145608602646	24.699139775122003
31	24.86955606358236	25.00302896083802	25.16838042953644	24.959034546043185
32	23.988873286154373	24.956750411640744	25.708826491192177	25.345549811012706
33	24.163261982271145	24.577186860069954	26.068329384098053	25.191221773560844
34	24.850190576257315	24.227614986305124	25.78380568570708	25.138388751730478
35	24.4769828769368	24.394853348640343	26.258508400649088	24.869655373773767
36	24.087786236799193	24.256117011239926	26.345404818133243	25.310691933827634
37	24.645512271760353	22.97193692611123	26.51999213463284	25.862558667495577
38	23.68140893354758	23.382683877784906	27.465425156860444	25.47048203180707
39	23.56620911151144	23.166584901275737	27.7487571329545	25.518448854258324
40	23.189724175874378	22.94323628079361	28.535095228542545	25.331944314789475
41	22.50219972073974	22.854750900246884	29.246255509232867	25.396793869780502
42	22.760207598024124	22.454630139054128	29.072264053881735	25.71289820904001
43	21.83046558603937	22.291165563992507	29.748069906429937	26.130298943538182
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	139.0
1	133.0
2	127.0
3	304.0
4	481.0
5	481.0
6	627.0
7	773.0
8	733.5
9	694.0
10	1013.5
11	1333.0
12	1333.0
13	2148.0
14	2963.0
15	4645.0
16	6327.0
17	6218.5
18	6110.0
19	6110.0
20	6964.0
21	7818.0
22	8279.0
23	8740.0
24	10083.0
25	11426.0
26	11426.0
27	12728.5
28	14031.0
29	17256.0
30	20481.0
31	23502.5
32	26524.0
33	26524.0
34	29520.5
35	32517.0
36	36297.0
37	40077.0
38	44062.0
39	48047.0
40	48047.0
41	52008.0
42	55969.0
43	56243.5
44	56518.0
45	60019.0
46	63520.0
47	63520.0
48	65286.0
49	67052.0
50	70589.0
51	74126.0
52	78102.5
53	82079.0
54	82079.0
55	76829.5
56	71580.0
57	68863.0
58	66146.0
59	60753.0
60	55360.0
61	55360.0
62	52302.0
63	49244.0
64	44584.0
65	39924.0
66	34837.0
67	29750.0
68	29750.0
69	25729.5
70	21709.0
71	19061.0
72	16413.0
73	13701.0
74	10989.0
75	10989.0
76	8978.0
77	6967.0
78	5818.5
79	4670.0
80	3741.0
81	2812.0
82	2812.0
83	2238.0
84	1664.0
85	1312.0
86	960.0
87	758.0
88	556.0
89	556.0
90	409.0
91	262.0
92	158.0
93	54.0
94	30.0
95	6.0
96	6.0
97	5.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1006946.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.86679625974399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34569118131363	57.307019502845336
2	6.939809328675314	9.003263956093049
3	1.7975810398969787	3.498099692261282
4	0.7925717996617315	2.0564637379950472
5	0.4436429780584587	1.438884933489206
6	0.29726187558674755	1.1569455311684953
7	0.19637469719383607	0.891673823140904
8	0.1471530219050328	0.7636276072735527
9	0.11476712033022166	0.6700117870600211
>10	0.7495324899385754	9.469036889930308
>50	0.1066936883250596	4.857954930525187
>100	0.06723978472830103	7.616594088107745
>500	0.0012225413717323417	0.5084271406557184
>1k	4.5845301439962824E-4	0.7619963794541834
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3142	0.31203262141167454	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2285	0.22692378737290778	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2260	0.22444103258764622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.931019141046293E-5	0.0	9.931019141046293E-5	0.0
3	0.0	9.931019141046293E-5	0.0	9.931019141046293E-5	0.0
4	0.0	9.931019141046293E-5	0.0	9.931019141046293E-5	0.0
5	0.0	9.931019141046293E-5	0.0	9.931019141046293E-5	0.0
6	0.0	9.931019141046293E-5	0.0	9.931019141046293E-5	9.931019141046293E-5
7	0.0	9.931019141046293E-5	0.0	9.931019141046293E-5	9.931019141046293E-5
8	0.0	9.931019141046293E-5	0.0	9.931019141046293E-5	9.931019141046293E-5
9	0.0	9.931019141046293E-5	0.0	2.979305742313888E-4	9.931019141046293E-5
10	0.0	9.931019141046293E-5	0.0	2.979305742313888E-4	9.931019141046293E-5
11	0.0	9.931019141046293E-5	0.0	3.972407656418517E-4	9.931019141046293E-5
12	0.0	9.931019141046293E-5	0.0	3.972407656418517E-4	9.931019141046293E-5
13	0.0	9.931019141046293E-5	0.0	3.972407656418517E-4	9.931019141046293E-5
14	0.0	9.931019141046293E-5	0.0	3.972407656418517E-4	9.931019141046293E-5
15	0.0	9.931019141046293E-5	0.0	4.965509570523146E-4	9.931019141046293E-5
16	0.0	9.931019141046293E-5	0.0	6.951713398732405E-4	9.931019141046293E-5
17	0.0	9.931019141046293E-5	0.0	7.944815312837034E-4	9.931019141046293E-5
18	0.0	9.931019141046293E-5	0.0	8.937917226941663E-4	9.931019141046293E-5
19	0.0	9.931019141046293E-5	0.0	0.001390342679746481	9.931019141046293E-5
20	0.0	9.931019141046293E-5	0.0	0.0015889630625674069	9.931019141046293E-5
21	0.0	9.931019141046293E-5	0.0	0.0019862038282092583	9.931019141046293E-5
22	0.0	9.931019141046293E-5	0.0	0.002681375168082499	9.931019141046293E-5
23	0.0	9.931019141046293E-5	0.0	0.0029793057423138877	9.931019141046293E-5
24	0.0	9.931019141046293E-5	0.0	0.004568268804881295	9.931019141046293E-5
25	0.0	9.931019141046293E-5	0.0	0.005958611484627775	9.931019141046293E-5
26	0.0	9.931019141046293E-5	0.0	0.008540676461299811	1.9862038282092586E-4
27	0.0	9.931019141046293E-5	0.0	0.027210992446466842	1.9862038282092586E-4
28	0.0	9.931019141046293E-5	0.0	0.11083017361407663	1.9862038282092586E-4
29	0.0	9.931019141046293E-5	0.0	0.23457067211151342	1.9862038282092586E-4
30	0.0	9.931019141046293E-5	0.0	0.3841318203756706	1.9862038282092586E-4
31	0.0	9.931019141046293E-5	0.0	0.8341062976564781	1.9862038282092586E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	865	0.0	26.092484	1
GTCTATA	45	0.0038254834	20.555557	1
ATTACGT	55	5.1424844E-4	20.181818	17
ATACACT	105	2.2586391E-8	19.38095	4
ATAGAGT	50	0.007034292	18.499998	4
TTTCGAG	95	3.605488E-6	17.526316	12
TGATAGA	85	2.722573E-5	17.411764	2
CTAGACT	65	0.0015797503	17.076923	4
CTTATAC	930	0.0	16.908602	37
AAGACCG	90	4.4450753E-5	16.444445	5
TTATAGT	90	4.4450753E-5	16.444445	4
TACGACG	80	3.3820453E-4	16.1875	5
AGGTCGC	265	0.0	16.056604	10
CGGTTTA	105	9.340716E-6	15.857142	15
TAACGCC	105	9.340716E-6	15.857142	4
ATAACGC	70	0.0025921944	15.857142	3
GTAAGGT	70	0.0025921944	15.857142	4
CCTATAA	70	0.0025921944	15.857142	1
TACACTG	280	0.0	15.857142	5
GTATCAA	1415	0.0	15.819788	2
>>END_MODULE