FastQCFastQC Report
Fri 10 Feb 2017
ERR1632328.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632328.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118404
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATTG250.00547669929.622
CGTATGC250.00547669929.631
TTTGGAT250.00547669929.62
TCGTATG250.00547669929.630
GCCGTCT358.822295E-426.42857236
GGTATCA806.8522786E-720.81251
CAGAACT555.1057426E-420.1818184
CTCTTAT2000.018.537
GAGAGAG500.006998583718.57
CGAGCCC500.006998583718.55
CCTGATC500.006998583718.53
GTATCAA1051.6359283E-414.0952382
CCGAGCC800.00625473113.8754
GATCTGG800.00625473113.8751
TCTTATA1102.4273149E-413.45454537
TCTCTTA3001.8189894E-1212.33333336
CAACGCA1401.3780779E-411.8928584
GTCTCTT4300.011.61627937
ATCAACG1451.9103354E-411.48275852
AACGCAG1451.9103354E-411.48275855
Produced by FastQC (version 0.11.5)