##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632328.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	118404
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.942442822877606	31.0	31.0	34.0	30.0	34.0
2	32.12497888584845	33.0	31.0	34.0	30.0	34.0
3	32.145493395493396	34.0	31.0	34.0	30.0	34.0
4	35.86430357082531	37.0	35.0	37.0	35.0	37.0
5	35.47971352319178	37.0	35.0	37.0	33.0	37.0
6	35.467475760954024	37.0	35.0	37.0	33.0	37.0
7	35.45490861795209	37.0	35.0	37.0	33.0	37.0
8	35.417131178000744	37.0	35.0	37.0	33.0	37.0
9	37.10055403533664	39.0	37.0	39.0	33.0	39.0
10	36.91022262761393	39.0	37.0	39.0	33.0	39.0
11	37.05872267828789	39.0	37.0	39.0	33.0	39.0
12	36.98453599540556	39.0	37.0	39.0	33.0	39.0
13	37.063139758791934	39.0	37.0	39.0	33.0	39.0
14	38.205474477213606	40.0	38.0	41.0	34.0	41.0
15	38.18948684166075	40.0	38.0	41.0	34.0	41.0
16	38.0701581027668	40.0	38.0	41.0	33.0	41.0
17	38.1106297084558	40.0	38.0	41.0	33.0	41.0
18	38.12189621972231	40.0	38.0	41.0	33.0	41.0
19	38.15912469173339	40.0	38.0	41.0	34.0	41.0
20	38.12610215871086	40.0	38.0	41.0	34.0	41.0
21	38.08967602445863	40.0	38.0	41.0	34.0	41.0
22	38.0540184453228	40.0	37.0	41.0	34.0	41.0
23	37.996300800648626	40.0	37.0	41.0	33.0	41.0
24	37.91893010371271	40.0	37.0	41.0	33.0	41.0
25	37.84056281882369	39.0	37.0	41.0	33.0	41.0
26	37.726749096314315	39.0	37.0	40.0	33.0	41.0
27	37.61986926117361	39.0	37.0	40.0	33.0	41.0
28	37.52182358704098	39.0	37.0	40.0	32.0	41.0
29	37.36169386169386	39.0	37.0	40.0	32.0	41.0
30	37.309753048883486	39.0	36.0	40.0	32.0	41.0
31	37.22541468193642	39.0	36.0	40.0	31.0	41.0
32	37.12362758014932	39.0	36.0	40.0	31.0	41.0
33	36.96105705888315	39.0	36.0	40.0	31.0	41.0
34	36.92731664470795	39.0	36.0	40.0	31.0	41.0
35	36.91669200364853	39.0	36.0	40.0	31.0	41.0
36	36.89145636971724	39.0	36.0	40.0	31.0	41.0
37	36.847285564676866	39.0	36.0	40.0	31.0	41.0
38	36.78315766359245	39.0	35.0	40.0	31.0	41.0
39	36.59507280159454	39.0	35.0	40.0	30.0	41.0
40	36.58791088138914	39.0	35.0	40.0	30.0	41.0
41	36.586652477956825	38.0	35.0	40.0	30.0	41.0
42	36.48492449579406	38.0	35.0	40.0	30.0	41.0
43	35.52309888179453	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	8.0
20	22.0
21	35.0
22	55.0
23	128.0
24	178.0
25	292.0
26	487.0
27	705.0
28	1003.0
29	1369.0
30	1783.0
31	2368.0
32	3206.0
33	4444.0
34	6174.0
35	9068.0
36	13988.0
37	22388.0
38	33110.0
39	17587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08131482044526	18.07962568832134	12.271544880240533	26.56751461099287
2	21.066011283402588	19.66656531873923	31.79960136481876	27.467822033039425
3	21.778825039694606	20.897098071011115	27.92557683862032	29.398500050673963
4	17.011249619945275	15.192898888551062	31.24303232998885	36.55281916151482
5	17.5931556366339	33.46508563899868	32.038613560352694	16.90314516401473
6	37.84331610418567	32.12982669504408	14.194621803317457	15.832235397452788
7	31.580858754771796	27.618154792067834	19.719772980642546	21.08121347251782
8	28.410357758183846	31.237964933617107	18.690246951116514	21.66143035708253
9	27.073409682105332	13.624539711496233	18.34819769602378	40.95385291037465
10	18.639572987399074	24.91638795986622	31.7075436640654	24.7364953886693
11	38.42944495118408	20.001858045336306	19.543258673693455	22.025438329786155
12	23.401236444714705	23.456133238741934	27.14519779737171	25.99743251917165
13	32.89162528292963	17.957163609337524	21.913955609607783	27.237255498125062
14	24.527887571365834	19.16742677612243	22.64028242289112	33.66440322962062
15	27.309043613391438	25.559947299077734	19.87939596635249	27.251613121178337
16	27.550589507111244	24.01016857538597	21.9274686665991	26.511773250903687
17	25.942535725144424	24.436674436674437	23.05749805749806	26.563291780683084
18	26.17563595824465	23.414749501706023	23.758487888922673	26.65112665112665
19	26.99739873652917	23.47386912604304	23.505118070335463	26.023614067092325
20	27.053140096618357	23.24836998750042	23.37505489679403	26.323435019087192
21	27.40532414445458	23.247525421438464	22.925745751832707	26.421404682274247
22	27.60464173507652	23.311712442147225	22.317658187223405	26.76598763555285
23	26.93658998006824	23.061720887807844	22.98655450829364	27.015134623830278
24	26.9830411134759	23.45782237086585	22.44434309651701	27.114793419141247
25	27.02189115232593	22.96375122462079	22.95783926218709	27.05651836086619
26	26.853822505996416	23.299043951217865	23.118306813958988	26.728826728826725
27	26.809905070774636	23.01442518833823	23.128441606702477	27.047228134184653
28	26.68237559541907	22.883517448734843	23.492449579406102	26.941657376439988
29	26.32850241545894	23.35816357555488	23.67909867909868	26.634235329887503
30	26.338637208202424	23.34887334887335	23.527076787946353	26.785412654977875
31	26.98219654741394	23.25597108205804	22.8902739772305	26.871558393297523
32	26.534576534576537	23.136887267322052	23.226411269889528	27.102124928211886
33	26.098780446606533	23.097192662410055	23.728928076754162	27.07509881422925
34	26.529509138204787	22.49923989054424	23.64362690449647	27.327624066754503
35	26.162967467315294	22.85480220262829	24.06337623728928	26.918854092767138
36	26.34286003851221	22.547380156075807	23.918110874632614	27.191648930779365
37	26.014323840410796	21.971386101820883	24.826019391236784	27.18827066653154
38	25.261815479206785	22.092159048680788	25.505895071112462	27.14013040099997
39	25.202695854869766	21.511097598054118	26.01010101010101	27.276105536975102
40	24.795615013006316	21.53052261747914	26.80821593865072	26.86564643086382
41	24.131786088307827	21.623424884294447	27.10465862639776	27.14013040099997
42	24.03466099118273	21.854835985270768	27.29553055640012	26.81497246714638
43	23.494983277591974	21.369210499645284	27.954292084726866	27.18151413803588
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	5.0
3	6.0
4	7.0
5	7.0
6	7.0
7	7.0
8	9.0
9	11.0
10	20.5
11	30.0
12	30.0
13	45.5
14	61.0
15	84.0
16	107.0
17	117.0
18	127.0
19	127.0
20	167.5
21	208.0
22	235.0
23	262.0
24	349.0
25	436.0
26	436.0
27	558.5
28	681.0
29	903.5
30	1126.0
31	1412.0
32	1698.0
33	1698.0
34	2103.0
35	2508.0
36	2976.0
37	3444.0
38	3976.0
39	4508.0
40	4508.0
41	5042.5
42	5577.0
43	6191.5
44	6806.0
45	7227.0
46	7648.0
47	7648.0
48	8131.0
49	8614.0
50	8949.0
51	9284.0
52	9576.5
53	9869.0
54	9869.0
55	9816.5
56	9764.0
57	9539.5
58	9315.0
59	9123.0
60	8931.0
61	8931.0
62	8218.5
63	7506.0
64	6809.0
65	6112.0
66	5369.0
67	4626.0
68	4626.0
69	4018.0
70	3410.0
71	2840.0
72	2270.0
73	1831.0
74	1392.0
75	1392.0
76	1117.5
77	843.0
78	683.5
79	524.0
80	427.0
81	330.0
82	330.0
83	256.5
84	183.0
85	136.0
86	89.0
87	67.5
88	46.0
89	46.0
90	38.0
91	30.0
92	18.0
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	118404.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.88089924865656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.53895144038783	80.39867316668656
2	4.742997511122439	8.24151777800915
3	1.2990510372545128	3.385881668597164
4	0.5470004562290642	1.9009556610642604
5	0.2649998732285079	1.1511713643436379
6	0.18499990321431217	0.9643774771304322
7	0.12299993546268813	0.7480441500367546
8	0.08599995487187014	0.5977402731689568
9	0.05599997061414153	0.4378795024369463
>10	0.15499991866413207	2.0390936353560494
>50	9.999994752524647E-4	0.045178043902221784
>100	9.999994752524647E-4	0.08948727926786237
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	8.445660619573663E-4	0.0
22	0.0	0.0	0.0	0.002533698185872099	0.0
23	0.0	0.0	0.0	0.004222830309786832	0.0
24	0.0	0.0	0.0	0.00675652849565893	0.0
25	0.0	0.0	0.0	0.007601094557616297	0.0
26	0.0	0.0	0.0	0.010134792743488396	0.0
27	0.0	0.0	0.0	0.022803283672848892	0.0
28	0.0	0.0	0.0	0.07009898314246141	0.0
29	0.0	0.0	0.0	0.15371102327624067	0.0
30	0.0	0.0	0.0	0.2432350258437215	0.0
31	0.0	0.0	0.0	0.47126786257221037	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATTG	25	0.005476699	29.6	22
CGTATGC	25	0.005476699	29.6	31
TTTGGAT	25	0.005476699	29.6	2
TCGTATG	25	0.005476699	29.6	30
GCCGTCT	35	8.822295E-4	26.428572	36
GGTATCA	80	6.8522786E-7	20.8125	1
CAGAACT	55	5.1057426E-4	20.181818	4
CTCTTAT	200	0.0	18.5	37
GAGAGAG	50	0.0069985837	18.5	7
CGAGCCC	50	0.0069985837	18.5	5
CCTGATC	50	0.0069985837	18.5	3
GTATCAA	105	1.6359283E-4	14.095238	2
CCGAGCC	80	0.006254731	13.875	4
GATCTGG	80	0.006254731	13.875	1
TCTTATA	110	2.4273149E-4	13.454545	37
TCTCTTA	300	1.8189894E-12	12.333333	36
CAACGCA	140	1.3780779E-4	11.892858	4
GTCTCTT	430	0.0	11.616279	37
ATCAACG	145	1.9103354E-4	11.4827585	2
AACGCAG	145	1.9103354E-4	11.4827585	5
>>END_MODULE