FastQCFastQC Report
Fri 10 Feb 2017
ERR1632327.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632327.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536569
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCC451.321928E-424.6666664
GGTATCA2650.024.433961
TTATACT400.001929852223.1250024
CTTACAC400.001929852223.1250023
CTTATAC3700.022.037
GTCCTAT450.003823189820.5555551
CAACACT851.2427772E-619.5882364
TCGTTTA500.007030112718.530
GATAACG902.1478663E-618.511
GTTCAAA1103.8402504E-818.51
CCGGTCG701.2176604E-418.520
CGTTTAT500.007030112718.531
AATTCCG953.5991416E-617.5263165
CGATAAC852.718736E-517.41176410
GCCGTCT852.718736E-517.41176436
ATAACGA752.0646794E-417.26666612
GTATCAA3700.017.02
TCTTATA6550.016.66412237
ATACCGC1005.866046E-616.65000227
TCCGATA904.4388522E-516.4444458
Produced by FastQC (version 0.11.5)