FastQCFastQC Report
Fri 10 Feb 2017
ERR1632326.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632326.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698020
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10770.15429357325005014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9200.1318013810492536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACT358.865891E-426.428574
GGTATCA4900.023.7857151
CGTACCC400.001930431223.1250023
TACTATA1001.2876626E-820.352
GCAATAG555.140313E-420.1818183
CGTCACT1101.7516868E-920.18181820
TTTCGTC1103.8451617E-818.517
CTTATAC4800.018.537
TTTTCGT1103.8451617E-818.516
TATACTC752.0658015E-417.2666665
TTTTTCG1005.8711375E-616.65000215
GTATCAA7150.016.30072
TCGTCAC1251.654862E-716.27999919
TCTTATA8600.015.91860437
TTCGTCA1059.332505E-615.85714418
GTATTAG1201.9327545E-615.4166661
ATAATAC750.004102259414.83
AAGACCG750.004102259414.85
TAGCACC750.004102259414.84
TATACAG750.004102259414.85
Produced by FastQC (version 0.11.5)