##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632326.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	698020
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44853012807656	31.0	31.0	34.0	30.0	34.0
2	31.626249964184407	31.0	31.0	34.0	30.0	34.0
3	31.665671470731496	31.0	31.0	34.0	30.0	34.0
4	35.512241769576804	37.0	35.0	37.0	33.0	37.0
5	34.775267184321365	37.0	35.0	37.0	32.0	37.0
6	34.7285822755795	35.0	35.0	37.0	32.0	37.0
7	34.7284203891006	35.0	35.0	37.0	32.0	37.0
8	34.66448669092576	35.0	35.0	37.0	32.0	37.0
9	36.26116730179651	37.0	35.0	39.0	32.0	39.0
10	35.97935732500501	37.0	35.0	39.0	32.0	39.0
11	36.16253832268416	37.0	35.0	39.0	32.0	39.0
12	36.06623735709579	37.0	35.0	39.0	32.0	39.0
13	36.17227156814991	37.0	35.0	39.0	32.0	39.0
14	37.177305807856506	38.0	36.0	40.0	32.0	41.0
15	37.13872525142546	38.0	36.0	40.0	32.0	41.0
16	37.02454514197301	38.0	36.0	40.0	32.0	41.0
17	37.03849173376121	38.0	36.0	40.0	32.0	41.0
18	37.03125841666428	38.0	36.0	40.0	32.0	41.0
19	37.08854760608578	39.0	36.0	40.0	32.0	41.0
20	37.05160597117561	39.0	36.0	40.0	31.0	41.0
21	36.99930374487837	39.0	36.0	40.0	31.0	41.0
22	36.970460731784186	39.0	36.0	40.0	31.0	41.0
23	36.90118477980574	38.0	36.0	40.0	31.0	41.0
24	36.801401105985505	38.0	36.0	40.0	31.0	41.0
25	36.70943096186355	38.0	36.0	40.0	31.0	41.0
26	36.541975874616774	38.0	35.0	40.0	30.0	41.0
27	36.42638606343658	38.0	35.0	40.0	30.0	41.0
28	36.302233460359304	38.0	35.0	40.0	30.0	41.0
29	36.136381479040715	38.0	35.0	40.0	30.0	41.0
30	36.04221798802327	38.0	35.0	40.0	29.0	41.0
31	35.92426864559755	38.0	34.0	40.0	29.0	41.0
32	35.81187358528409	38.0	34.0	40.0	29.0	41.0
33	35.63946018738718	38.0	34.0	40.0	28.0	41.0
34	35.61267585456004	38.0	34.0	40.0	28.0	41.0
35	35.554959743273834	38.0	34.0	40.0	28.0	41.0
36	35.54122088192315	38.0	34.0	40.0	28.0	41.0
37	35.50661585627919	38.0	34.0	40.0	28.0	41.0
38	35.4175396120455	38.0	34.0	40.0	27.0	41.0
39	35.23616658548465	38.0	34.0	40.0	27.0	41.0
40	35.214819059625796	38.0	33.0	40.0	27.0	41.0
41	35.188328414658606	38.0	33.0	40.0	27.0	41.0
42	35.060604280679634	38.0	33.0	40.0	27.0	41.0
43	34.10690811151543	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	2.0
15	12.0
16	17.0
17	54.0
18	89.0
19	171.0
20	299.0
21	498.0
22	874.0
23	1393.0
24	2208.0
25	3257.0
26	4887.0
27	6963.0
28	9635.0
29	13048.0
30	17584.0
31	23617.0
32	31568.0
33	42548.0
34	57548.0
35	78354.0
36	104755.0
37	129527.0
38	125398.0
39	43710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82043494455746	18.92596200681929	12.818687143634852	26.434915904988394
2	20.20457866536775	20.432652359531247	33.10650124638263	26.256267728718374
3	21.103836566287498	21.877166843356925	28.54416778888857	28.474828801467005
4	16.318013810492534	15.792527434743992	32.52557233317097	35.363886421592504
5	16.683762642904217	34.3375547978568	32.67642760952408	16.302254949714907
6	36.27575141113435	33.26007850777914	15.048995730781353	15.41517435030515
7	30.578923240021776	28.67768831838629	20.549554454027106	20.193833987564826
8	27.852067276009286	32.330019197157675	19.35445975760007	20.463453769232974
9	27.119280249849574	14.028251339503168	19.07280593679264	39.779662473854614
10	18.11165869172803	25.623477837311253	31.757112976705535	24.50775049425518
11	37.82599352454085	20.63164379244148	20.402710524053752	21.139652158963926
12	23.00005730494828	24.199306610125785	27.78459069940689	25.016045385519043
13	31.830033523394746	18.631557835019056	23.082719692845476	26.455688948740725
14	23.916506690352712	19.680668175696972	23.509928082289903	32.89289705166041
15	26.605254863757487	26.38606343657775	20.747686312713103	26.260995386951663
16	26.497378298616088	24.792412824847425	22.813099911177332	25.897108965359163
17	25.185094982951778	25.012893613363513	24.082691040371337	25.71932036331337
18	25.450703418240167	23.87882868685711	24.930374487837025	25.7400934070657
19	26.013724535113607	24.2599065929343	24.68768803186155	25.038680840090542
20	26.211713131428898	23.890432938884274	24.410475344546	25.48737858514083
21	26.790206584338556	23.892008824962037	23.90848399759319	25.409300593106217
22	26.70267327583737	23.926248531560702	23.703618807484027	25.6674593851179
23	26.036789776797225	23.710781926019312	24.231254118793156	26.021174178390304
24	26.052978424686973	24.180969026675452	23.800893957193203	25.96515859144437
25	26.197386894358328	23.499613191599096	24.128391736626458	26.17460817741612
26	25.819460760436662	24.10160167330449	24.226526460559867	25.852411105698973
27	25.919601157559956	23.767227300077362	24.13999598865362	26.17317555370906
28	25.628348757915248	23.91793931405977	24.42680725480645	26.02690467321853
29	25.60743245179221	24.18268817512392	24.674078106644508	25.535801266439357
30	25.52734878656772	24.331251253545744	24.353170396263717	25.788229563622817
31	26.06931033494742	24.02982722558093	24.055757714678663	25.845104724792982
32	25.21145525916163	24.104466920718604	24.337554797856793	26.346523022262975
33	25.014182974699867	23.770092547491476	24.956018452193344	26.25970602561531
34	25.873613936563423	23.28772814532535	24.721354688977392	26.11730322913384
35	25.442680725480642	23.55118764505315	25.312025443397033	25.694106186069167
36	25.15859144437122	23.532706799232113	25.074496432766967	26.234205323629695
37	25.090112031173895	22.91166442222286	25.758287728145323	26.239935818457923
38	24.468496604681814	22.79648147617547	26.48749319503739	26.24752872410533
39	24.382968969370506	22.36053408211799	26.93533136586344	26.32116558264806
40	24.16076903240595	22.407094352597348	27.479728374545143	25.952408240451565
41	23.468525257155957	22.35795535944529	28.01194808171686	26.161571301681903
42	23.452193346895505	22.609810607145928	27.987593478696887	25.950402567261683
43	22.81080771324604	22.083894444285264	28.405346551674736	26.699951290793962
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	25.0
2	34.0
3	79.5
4	125.0
5	125.0
6	153.5
7	182.0
8	200.0
9	218.0
10	315.0
11	412.0
12	412.0
13	776.0
14	1140.0
15	1706.0
16	2272.0
17	2270.5
18	2269.0
19	2269.0
20	2680.0
21	3091.0
22	3104.0
23	3117.0
24	3849.0
25	4581.0
26	4581.0
27	5603.5
28	6626.0
29	8134.0
30	9642.0
31	11709.0
32	13776.0
33	13776.0
34	16294.0
35	18812.0
36	21750.5
37	24689.0
38	27718.5
39	30748.0
40	30748.0
41	33885.0
42	37022.0
43	39817.5
44	42613.0
45	44896.5
46	47180.0
47	47180.0
48	49073.5
49	50967.0
50	52310.0
51	53653.0
52	54524.0
53	55395.0
54	55395.0
55	54192.0
56	52989.0
57	51416.5
58	49844.0
59	48114.5
60	46385.0
61	46385.0
62	42538.0
63	38691.0
64	34669.0
65	30647.0
66	26901.0
67	23155.0
68	23155.0
69	20078.5
70	17002.0
71	14427.0
72	11852.0
73	9584.5
74	7317.0
75	7317.0
76	5903.0
77	4489.0
78	3649.5
79	2810.0
80	2362.0
81	1914.0
82	1914.0
83	1487.5
84	1061.0
85	859.5
86	658.0
87	519.5
88	381.0
89	381.0
90	285.5
91	190.0
92	114.5
93	39.0
94	26.5
95	14.0
96	14.0
97	7.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	698020.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.16307956051872
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.55098435731792	66.41376777614538
2	5.9977196700405315	8.896187221418081
3	1.6799446858359224	3.7376961417876045
4	0.8009694777640017	2.376094524198352
5	0.47218575780880706	1.750937496185919
6	0.29311228357647245	1.304286576422835
7	0.21687958460251514	1.1259120521550008
8	0.1519206078632163	0.9013520102273661
9	0.1167067536891685	0.7789799033189699
>10	0.6646629394498842	8.821827009994532
>50	0.03977885170597598	1.965924927896533
>100	0.014552913794420124	1.5397855020395936
>500	3.8807770079153745E-4	0.23226297181369043
>1k	1.9403885039576872E-4	0.15498588639612426
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1077	0.15429357325005014	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	920	0.1318013810492536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.4326237070571044E-4	0.0
10	0.0	0.0	0.0	1.4326237070571044E-4	0.0
11	0.0	0.0	0.0	1.4326237070571044E-4	0.0
12	0.0	0.0	0.0	1.4326237070571044E-4	0.0
13	0.0	0.0	0.0	1.4326237070571044E-4	0.0
14	0.0	0.0	0.0	2.865247414114209E-4	0.0
15	0.0	0.0	0.0	4.2978711211713133E-4	0.0
16	0.0	0.0	0.0	7.163118535285522E-4	0.0
17	0.0	0.0	0.0	0.001289361336351394	0.0
18	0.0	0.0	0.0	0.0015758860777628147	0.0
19	0.0	0.0	0.0	0.0017191484484685253	0.0
20	0.0	0.0	0.0	0.0018624108191742357	0.0
21	0.0	0.0	0.0	0.0024354603019970775	0.0
22	0.0	0.0	0.0	0.003008509784819919	0.0
23	0.0	0.0	0.0	0.003581559267642761	0.0
24	0.0	0.0	0.0	0.005730494828228417	0.0
25	0.0	0.0	0.0	0.007449643276696943	0.0
26	0.0	0.0	0.0	0.008882266983754047	0.0
27	0.0	0.0	0.0	0.02593048909773359	0.0
28	0.0	0.0	0.0	0.08037018996590356	0.0
29	0.0	0.0	0.0	0.15586945932781296	0.0
30	0.0	0.0	0.0	0.2627431878742729	0.0
31	0.0	0.0	0.0	0.5982636600670468	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAACT	35	8.865891E-4	26.42857	4
GGTATCA	490	0.0	23.785715	1
CGTACCC	40	0.0019304312	23.125002	3
TACTATA	100	1.2876626E-8	20.35	2
GCAATAG	55	5.140313E-4	20.181818	3
CGTCACT	110	1.7516868E-9	20.181818	20
TTTCGTC	110	3.8451617E-8	18.5	17
CTTATAC	480	0.0	18.5	37
TTTTCGT	110	3.8451617E-8	18.5	16
TATACTC	75	2.0658015E-4	17.266666	5
TTTTTCG	100	5.8711375E-6	16.650002	15
GTATCAA	715	0.0	16.3007	2
TCGTCAC	125	1.654862E-7	16.279999	19
TCTTATA	860	0.0	15.918604	37
TTCGTCA	105	9.332505E-6	15.857144	18
GTATTAG	120	1.9327545E-6	15.416666	1
ATAATAC	75	0.0041022594	14.8	3
AAGACCG	75	0.0041022594	14.8	5
TAGCACC	75	0.0041022594	14.8	4
TATACAG	75	0.0041022594	14.8	5
>>END_MODULE