FastQCFastQC Report
Fri 10 Feb 2017
ERR1632325.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632325.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598685
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGAC352.386411E-531.7142853
GGTATCA2100.029.071431
ATAGCAC358.864412E-426.428573
TAGGACA400.001930112423.1254
TTCTATA656.895072E-519.9230772
GTATAGA759.253612E-619.7333341
GTATAGG851.2432683E-619.5882361
GTGTACA801.6145628E-518.51
TATAGAA701.2179595E-418.52
GTAAGAG609.2297705E-418.53
TATACTG701.2179595E-418.55
TATAGGT500.007031041718.4999984
GCGTCGC500.007031041718.49999828
GTATTAG852.719587E-517.4117641
TATTAGC752.0651816E-417.2666662
TAGCGCG1107.7922414E-716.8181824
CGCGCGT1107.7922414E-716.8181827
GTATCAA3750.016.2799992
CATCTAA1151.2396868E-616.08695625
TAGAATG1353.9625957E-715.0740754
Produced by FastQC (version 0.11.5)