##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632325.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	598685
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84162121984015	31.0	31.0	34.0	30.0	34.0
2	32.0252202744348	33.0	31.0	34.0	30.0	34.0
3	32.05611799193232	33.0	31.0	34.0	30.0	34.0
4	35.80605994805281	37.0	35.0	37.0	35.0	37.0
5	35.34705228960138	37.0	35.0	37.0	33.0	37.0
6	35.323455573465175	37.0	35.0	37.0	32.0	37.0
7	35.315843891194866	37.0	35.0	37.0	32.0	37.0
8	35.27282126660932	37.0	35.0	37.0	32.0	37.0
9	36.94699215781254	39.0	37.0	39.0	33.0	39.0
10	36.70384425866691	38.0	37.0	39.0	32.0	39.0
11	36.88155373861046	38.0	37.0	39.0	33.0	39.0
12	36.79740264078772	38.0	37.0	39.0	33.0	39.0
13	36.88190283705121	39.0	37.0	39.0	33.0	39.0
14	38.018747755497465	39.0	37.0	40.0	33.0	41.0
15	37.9843774271946	39.0	37.0	40.0	33.0	41.0
16	37.87762178775149	39.0	37.0	40.0	33.0	41.0
17	37.886108721614875	39.0	37.0	40.0	33.0	41.0
18	37.90281366661934	39.0	37.0	40.0	33.0	41.0
19	37.953872236651996	39.0	37.0	40.0	33.0	41.0
20	37.91376099284265	39.0	37.0	40.0	33.0	41.0
21	37.87193599305144	39.0	37.0	40.0	33.0	41.0
22	37.83720654434302	39.0	37.0	40.0	33.0	41.0
23	37.78538797531256	39.0	37.0	40.0	33.0	41.0
24	37.70421841201968	39.0	37.0	40.0	33.0	41.0
25	37.607635066854854	39.0	37.0	40.0	33.0	41.0
26	37.47902486282435	39.0	37.0	40.0	32.0	41.0
27	37.38422375706757	39.0	37.0	40.0	32.0	41.0
28	37.29367029406115	39.0	36.0	40.0	32.0	41.0
29	37.118362745016164	39.0	36.0	40.0	31.0	41.0
30	37.0598578551325	39.0	36.0	40.0	31.0	41.0
31	36.94928384709822	39.0	36.0	40.0	31.0	41.0
32	36.86973283112154	39.0	36.0	40.0	31.0	41.0
33	36.70040171375598	39.0	36.0	40.0	30.0	41.0
34	36.68687373159508	38.0	35.0	40.0	30.0	41.0
35	36.63815696067214	38.0	35.0	40.0	30.0	41.0
36	36.62659662426819	38.0	35.0	40.0	30.0	41.0
37	36.597998947693696	38.0	35.0	40.0	30.0	41.0
38	36.52293944227766	38.0	35.0	40.0	30.0	41.0
39	36.33327208799285	38.0	35.0	40.0	30.0	41.0
40	36.33532826110559	38.0	35.0	40.0	30.0	41.0
41	36.34003023292716	38.0	35.0	40.0	30.0	41.0
42	36.21053976632119	38.0	35.0	40.0	30.0	41.0
43	35.25522603706457	38.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	2.0
15	0.0
16	4.0
17	15.0
18	30.0
19	58.0
20	123.0
21	240.0
22	434.0
23	696.0
24	1093.0
25	1773.0
26	2493.0
27	3652.0
28	5136.0
29	7022.0
30	9952.0
31	13314.0
32	18011.0
33	24658.0
34	35686.0
35	51707.0
36	77512.0
37	118481.0
38	155463.0
39	71128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04149928593501	17.912758796362027	11.941338099334374	27.10440381836859
2	20.563735520348764	19.875059505416036	31.941839197574684	27.619365776660516
3	21.097071080785387	20.988165729891346	28.488437158104844	29.42632603121842
4	16.316426835481096	15.086898786507094	32.08966317846614	36.50701119954567
5	17.153093864052046	33.78153787049951	32.691983263318775	16.373385002129666
6	37.57802517183494	32.66208440164694	14.47906662101105	15.280823805507069
7	31.939500739119904	28.20832324177155	19.753960764007783	20.09821525510076
8	29.328110776117654	31.30711475984867	18.703992917811537	20.660781546222136
9	27.776710624117857	13.267745141434977	17.874508297351696	41.081035937095464
10	18.136415644287062	25.35055997728355	31.648362661499785	24.864661716929604
11	39.19807578275721	19.61732797714992	19.61498951869514	21.56960672139773
12	23.874491594077018	23.288707751154615	27.250390439045574	25.586410215722793
13	33.15382880813784	17.836591863834904	21.835689887002346	27.17388944102491
14	24.6241345615808	18.490358034692704	22.654985509909217	34.23052189381728
15	27.53334391207396	25.272221619048413	19.986470347511627	27.207964121365997
16	27.575770229753545	23.720988499795386	21.721606520958435	26.98163474949264
17	26.18705997310773	24.145752774831504	23.21387708060165	26.453310171459115
18	26.642892339043065	23.028804797180488	23.683907230012444	26.644395633764002
19	27.14933562724972	23.22757376583679	23.548944770622278	26.07414583629121
20	27.40305836959336	22.960321371004785	23.251125383131363	26.385494876270492
21	27.886618171492522	22.944954358301946	22.610053701028086	26.558373769177447
22	27.954433466681145	23.01878283237429	22.468911030007433	26.557872670937137
23	27.54436807336078	22.927415919891097	22.602704260170206	26.925511746577918
24	27.252561864753584	23.07173221310038	22.527539524123704	27.148166398022333
25	27.254566257714824	22.490625287087536	22.929420312852336	27.325388142345307
26	27.00552043228075	22.805314982002223	23.064382772242496	27.12478181347453
27	26.828632753451316	22.899855516674045	23.111318974084867	27.160192755789776
28	26.876070053533997	22.944119194568096	23.2213935542063	26.95841719769161
29	26.7629888839707	23.12484862657324	23.401955953464675	26.710206535991382
30	26.63303740698364	23.361534028746338	23.046677301084877	26.95875126318515
31	27.07066320352105	23.247951760942733	22.806985309469923	26.874399726066294
32	26.280431278552157	23.28720445643368	23.208364999958242	27.223999265055916
33	26.18806216958835	23.051354217994437	23.720988499795386	27.03959511262183
34	26.69199996659345	22.61873940386013	23.600223823880672	27.08903680566575
35	26.13761828006381	22.877807194100402	24.225761460534336	26.75881306530145
36	26.354593818118044	22.73466013011851	23.953331050552464	26.95741500121099
37	26.02771073268914	22.146203763247783	24.521743487810785	27.304342016252285
38	25.319157820890787	22.38038367421933	25.208749175275813	27.091709329614073
39	25.364423695265458	22.042476427503612	25.634181581299014	26.958918295931916
40	25.023676891854645	21.708410933963602	26.396017939317	26.871894234864747
41	24.563501674503286	21.45168160217811	26.842830536926765	27.141986186391843
42	24.114016552945206	21.466547516640638	27.42059680800421	26.99883912240995
43	23.719986303314766	20.76500998020662	27.8922972848827	27.622706431595915
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	5.5
2	8.0
3	17.0
4	26.0
5	26.0
6	38.5
7	51.0
8	45.5
9	40.0
10	65.0
11	90.0
12	90.0
13	162.5
14	235.0
15	392.5
16	550.0
17	622.5
18	695.0
19	695.0
20	913.5
21	1132.0
22	1319.0
23	1506.0
24	1961.5
25	2417.0
26	2417.0
27	3185.5
28	3954.0
29	5051.5
30	6149.0
31	7597.0
32	9045.0
33	9045.0
34	11057.5
35	13070.0
36	15147.5
37	17225.0
38	19908.5
39	22592.0
40	22592.0
41	25402.0
42	28212.0
43	30629.5
44	33047.0
45	35359.5
46	37672.0
47	37672.0
48	39840.5
49	42009.0
50	44187.5
51	46366.0
52	47445.5
53	48525.0
54	48525.0
55	48997.5
56	49470.0
57	48357.5
58	47245.0
59	45840.0
60	44435.0
61	44435.0
62	41730.5
63	39026.0
64	34908.5
65	30791.0
66	27320.5
67	23850.0
68	23850.0
69	20514.0
70	17178.0
71	14680.0
72	12182.0
73	9942.0
74	7702.0
75	7702.0
76	6319.5
77	4937.0
78	4018.5
79	3100.0
80	2581.5
81	2063.0
82	2063.0
83	1590.0
84	1117.0
85	823.5
86	530.0
87	397.5
88	265.0
89	265.0
90	199.0
91	133.0
92	82.0
93	31.0
94	21.0
95	11.0
96	11.0
97	5.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	598685.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.8126381739191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.85314567559931	70.81563458919452
2	6.1295212296271195	9.661674776999158
3	1.6561777394909536	3.9158321080259926
4	0.7487670555737708	2.3604922810994546
5	0.39509547847453574	1.5569258494582514
6	0.26218582876680535	1.2398134114156403
7	0.1767879998637737	0.9753190066728118
8	0.13792920263325797	0.8696451472601704
9	0.09804230237061265	0.6954275252225758
>10	0.5291704801252214	7.091155718748868
>50	0.011264231154168027	0.5422053509748531
>100	0.001912776320510728	0.27587423492770413
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	1.6703274677000427E-4
8	0.0	0.0	0.0	0.0	1.6703274677000427E-4
9	0.0	0.0	0.0	0.0	1.6703274677000427E-4
10	0.0	0.0	0.0	1.6703274677000427E-4	1.6703274677000427E-4
11	0.0	0.0	0.0	1.6703274677000427E-4	1.6703274677000427E-4
12	0.0	1.6703274677000427E-4	0.0	1.6703274677000427E-4	1.6703274677000427E-4
13	0.0	1.6703274677000427E-4	0.0	1.6703274677000427E-4	1.6703274677000427E-4
14	0.0	1.6703274677000427E-4	0.0	1.6703274677000427E-4	1.6703274677000427E-4
15	0.0	1.6703274677000427E-4	0.0	1.6703274677000427E-4	1.6703274677000427E-4
16	0.0	1.6703274677000427E-4	0.0	3.3406549354000854E-4	1.6703274677000427E-4
17	0.0	1.6703274677000427E-4	0.0	6.681309870800171E-4	1.6703274677000427E-4
18	0.0	1.6703274677000427E-4	0.0	6.681309870800171E-4	1.6703274677000427E-4
19	0.0	1.6703274677000427E-4	1.6703274677000427E-4	8.351637338500213E-4	1.6703274677000427E-4
20	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.0010021964806200255	3.3406549354000854E-4
21	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.00116922922739003	3.3406549354000854E-4
22	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.0018373602144700469	3.3406549354000854E-4
23	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.00233845845478006	3.3406549354000854E-4
24	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.0028395566950900723	3.3406549354000854E-4
25	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.0036747204289400938	3.3406549354000854E-4
26	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.00467691690956012	3.3406549354000854E-4
27	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.017705471157620452	5.010982403100127E-4
28	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.06564386948061167	5.010982403100127E-4
29	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.1331250991756934	5.010982403100127E-4
30	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.22065025848317563	5.010982403100127E-4
31	0.0	1.6703274677000427E-4	1.6703274677000427E-4	0.47253564061234204	5.010982403100127E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGAC	35	2.386411E-5	31.714285	3
GGTATCA	210	0.0	29.07143	1
ATAGCAC	35	8.864412E-4	26.42857	3
TAGGACA	40	0.0019301124	23.125	4
TTCTATA	65	6.895072E-5	19.923077	2
GTATAGA	75	9.253612E-6	19.733334	1
GTATAGG	85	1.2432683E-6	19.588236	1
GTGTACA	80	1.6145628E-5	18.5	1
TATAGAA	70	1.2179595E-4	18.5	2
GTAAGAG	60	9.2297705E-4	18.5	3
TATACTG	70	1.2179595E-4	18.5	5
TATAGGT	50	0.0070310417	18.499998	4
GCGTCGC	50	0.0070310417	18.499998	28
GTATTAG	85	2.719587E-5	17.411764	1
TATTAGC	75	2.0651816E-4	17.266666	2
TAGCGCG	110	7.7922414E-7	16.818182	4
CGCGCGT	110	7.7922414E-7	16.818182	7
GTATCAA	375	0.0	16.279999	2
CATCTAA	115	1.2396868E-6	16.086956	25
TAGAATG	135	3.9625957E-7	15.074075	4
>>END_MODULE