FastQCFastQC Report
Fri 10 Feb 2017
ERR1632324.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632324.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52054
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1030.1978714411956814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT830.1594498021285588No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT740.14216006454835362RNA PCR Primer, Index 17 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT650.12487032696814847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA753.6009442E-722.1999991
CTTATAC500.006947225418.537
GTATCAA1306.7520086E-512.8076932
CAACGCA1359.612177E-512.3333346
AACGCAG1359.612177E-512.3333347
ATCAACG1401.3497441E-411.8928574
TCAACGC1401.3497441E-411.8928575
TGTCTCT3002.0008883E-1111.71666637
TCTCTTA1451.8712849E-411.482758537
TATCAAC1502.5636796E-411.0999993
TACATGG1200.007008641510.79166716
GTACATG1250.00948820310.36000115
AGTACAT1250.00948820310.36000114
CTGTCTC3452.5465852E-1010.18840636
ACGCAGA1656.1841006E-410.0909098
CAGAGTA1800.00137299059.2511
AGAGTAC1850.0017626499.012
CGCAGAG1850.0017626499.09
GCAGAGT1850.0017626499.010
GTCTCTT1900.00224643088.76315836
Produced by FastQC (version 0.11.5)