FastQCFastQC Report
Fri 10 Feb 2017
ERR1632323.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632323.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976400
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48840.16409084800430052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38820.13042601800833223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35620.11967477489584734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18450.023.2628731
AACGCTA1401.873559E-917.17857233
TACGTTA1452.9849616E-916.58620819
CTTATAC17350.016.3141237
TTAACGG700.002593792315.85714235
TCTTATA29450.015.57894837
GTATACG1001.0942912E-414.81
CTTAACG1651.6480044E-814.57575730
TAACCCG3950.014.51898728
TAGGACA9000.014.3888894
GTATCAA29650.014.2883642
ACGTTAA1702.4410838E-814.147058520
TAACGCT1702.4410838E-814.147058532
CGACCAT2900.014.03448410
GCTTAGG8350.013.9580841
TTAGGAC10200.013.7843153
AATTACG1753.5719495E-813.74285716
GACCATA3250.013.66153911
TCTAGCG2202.8558134E-1013.45454528
CTTAGGA9850.013.3350262
Produced by FastQC (version 0.11.5)