##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632323.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2976400
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.610001679881734	34.0	31.0	34.0	31.0	34.0
2	32.769799422120684	34.0	31.0	34.0	31.0	34.0
3	32.77895813734713	34.0	31.0	34.0	31.0	34.0
4	36.284901222953906	37.0	37.0	37.0	35.0	37.0
5	36.17833322134122	37.0	35.0	37.0	35.0	37.0
6	36.210925278860365	37.0	36.0	37.0	35.0	37.0
7	36.19926891546835	37.0	36.0	37.0	35.0	37.0
8	36.18093435022175	37.0	36.0	37.0	35.0	37.0
9	37.93247345786857	39.0	38.0	39.0	35.0	39.0
10	37.85141210858755	39.0	38.0	39.0	35.0	39.0
11	37.966445370245935	39.0	38.0	39.0	35.0	39.0
12	37.9310875554361	39.0	38.0	39.0	35.0	39.0
13	37.95563869103615	39.0	38.0	39.0	35.0	39.0
14	39.32206524660664	40.0	39.0	41.0	36.0	41.0
15	39.34335203601666	40.0	39.0	41.0	37.0	41.0
16	39.226108385969624	40.0	39.0	41.0	36.0	41.0
17	39.280226784034404	40.0	39.0	41.0	36.0	41.0
18	39.298291896250504	40.0	39.0	41.0	36.0	41.0
19	39.35675816422524	40.0	39.0	41.0	37.0	41.0
20	39.33332280607445	40.0	39.0	41.0	36.0	41.0
21	39.307065582582986	40.0	39.0	41.0	36.0	41.0
22	39.29033160865475	40.0	39.0	41.0	36.0	41.0
23	39.24991365407875	40.0	39.0	41.0	36.0	41.0
24	39.200137078349684	40.0	39.0	41.0	36.0	41.0
25	39.14027482865206	40.0	39.0	41.0	36.0	41.0
26	39.05744053218653	40.0	39.0	41.0	36.0	41.0
27	38.99019453030507	40.0	39.0	41.0	36.0	41.0
28	38.921668458540516	40.0	38.0	41.0	35.0	41.0
29	38.789675110872196	40.0	38.0	41.0	35.0	41.0
30	38.74664863593603	40.0	38.0	41.0	35.0	41.0
31	38.673387985485824	40.0	38.0	41.0	35.0	41.0
32	38.60308023115173	40.0	38.0	41.0	35.0	41.0
33	38.47399879048515	40.0	38.0	41.0	34.0	41.0
34	38.4753625184787	40.0	38.0	41.0	34.0	41.0
35	38.446075460287595	40.0	38.0	41.0	34.0	41.0
36	38.4209397258433	40.0	38.0	41.0	34.0	41.0
37	38.39562256417148	40.0	38.0	41.0	34.0	41.0
38	38.32627973390673	40.0	38.0	41.0	34.0	41.0
39	38.14445135062492	40.0	38.0	41.0	34.0	41.0
40	38.1548770326569	40.0	38.0	41.0	34.0	41.0
41	38.15537158984007	40.0	38.0	41.0	34.0	41.0
42	38.03060038973256	40.0	38.0	41.0	34.0	41.0
43	37.19668357747614	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	3.0
13	3.0
14	4.0
15	7.0
16	13.0
17	23.0
18	53.0
19	122.0
20	214.0
21	418.0
22	723.0
23	1342.0
24	2107.0
25	3315.0
26	4911.0
27	7124.0
28	10573.0
29	15145.0
30	21194.0
31	29532.0
32	39878.0
33	55033.0
34	79460.0
35	117579.0
36	186597.0
37	342433.0
38	808953.0
39	1249635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62071630157237	18.277516462841014	12.474499395242574	26.62726784034404
2	18.74616986964118	20.486090579223223	34.443119204408006	26.32462034672759
3	19.85586614702325	21.871421851901626	30.07163015723693	28.201081843838192
4	14.944362316892889	15.542769789006854	34.36789410025534	35.14497379384491
5	15.442581642252385	35.28316086547507	33.878309367020556	15.395948125251982
6	34.818606370111546	34.67658916812256	15.65011423195807	14.85469022980782
7	29.814809837387447	30.02788603682301	20.835002015858084	19.32230210993146
8	27.508399408681626	33.041694664695605	20.036487031312998	19.41341889530977
9	27.010213680956863	14.077744926757157	19.242406934551806	39.669634457734176
10	17.047843031850558	26.485519419432872	32.825225104152665	23.641412444563905
11	36.430755274828655	21.090008063432332	21.162511759172155	21.316724902566857
12	22.273652734847467	24.837219459750035	28.72638086278726	24.16274694261524
13	31.18656766563634	18.961833086950676	24.178806612014515	25.672792635398466
14	23.410865475070555	20.0128006988308	24.48676253191775	32.08957129418089
15	25.935727724768178	26.76078484074721	21.75396452089773	25.549522913586888
16	25.720904448326838	25.619506786722212	23.206323074855533	25.453265690095417
17	24.505845988442413	25.760146485687407	24.708842897460016	25.025164628410163
18	24.367188549926087	24.518915468351025	25.939020292971378	25.174875688751513
19	25.378746136272007	24.727287998924876	25.65532186534068	24.23864399946244
20	25.87081709447655	24.208943690364197	25.382643461900283	24.53759575325897
21	25.890975675312454	24.730076602607177	24.39813197150921	24.980815750571157
22	25.971744389195	24.490424674102943	24.554730546969495	24.983100389732563
23	25.250436769251444	24.594745329928774	24.802849079424806	25.351968821394973
24	25.44963714554495	24.619070017470772	24.80987098508265	25.121421851901626
25	25.511960757962637	24.11513909420777	24.901962101868026	25.470938045961567
26	25.12266496438651	24.56316355328585	24.944496707431796	25.369674774895845
27	25.33389329391211	24.36789410025534	24.751377503023786	25.546835102808764
28	24.846324418760922	24.654213143394703	25.243952425749228	25.25551001209515
29	24.638119876360705	24.912142185190163	25.370380325225106	25.07935761322403
30	24.656968149442278	25.454676790753933	25.02378712538637	24.864567934417416
31	25.239282354522242	24.9694597500336	24.820521435290953	24.970736460153205
32	24.347231554898535	24.893428302647493	25.22271872060207	25.5366214218519
33	24.378779733906732	24.56941271334498	25.64144604219863	25.410361510549656
34	25.034303185055773	24.12273215965596	25.525970971643595	25.31699368364467
35	24.79811181292837	24.322839672087085	25.82710657169735	25.051941943287193
36	24.428773014379786	24.546532724096224	25.667988173632576	25.35670608789141
37	24.900181427227523	23.415770729740625	25.99700981050934	25.68703803252251
38	24.117289342830265	23.775836581104688	26.799690901760513	25.30718317430453
39	24.0427025937374	23.417249025668593	27.15609461093939	25.383953769654617
40	23.556444026340547	23.05284907942481	28.06336513909421	25.327341755140438
41	22.935156564977827	23.149106302916277	28.515589302513106	25.400147829592797
42	23.23911436634861	22.868129283698426	28.414426824351562	25.478329525601396
43	22.32203332885365	22.700409891143664	28.928201854589435	26.049354925413247
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	106.5
2	128.0
3	382.5
4	637.0
5	637.0
6	832.5
7	1028.0
8	1050.5
9	1073.0
10	1569.0
11	2065.0
12	2065.0
13	3542.5
14	5020.0
15	8067.5
16	11115.0
17	11410.0
18	11705.0
19	11705.0
20	14141.0
21	16577.0
22	18629.5
23	20682.0
24	25348.0
25	30014.0
26	30014.0
27	35283.5
28	40553.0
29	50788.0
30	61023.0
31	69946.0
32	78869.0
33	78869.0
34	89718.0
35	100567.0
36	111991.0
37	123415.0
38	135612.0
39	147809.0
40	147809.0
41	159500.0
42	171191.0
43	172819.5
44	174448.0
45	183277.5
46	192107.0
47	192107.0
48	198736.5
49	205366.0
50	212726.0
51	220086.0
52	231042.5
53	241999.0
54	241999.0
55	228504.0
56	215009.0
57	207176.0
58	199343.0
59	185432.0
60	171521.0
61	171521.0
62	159241.0
63	146961.0
64	131371.0
65	115781.0
66	101215.5
67	86650.0
68	86650.0
69	74456.5
70	62263.0
71	54354.0
72	46445.0
73	37814.5
74	29184.0
75	29184.0
76	23649.5
77	18115.0
78	14981.0
79	11847.0
80	9527.0
81	7207.0
82	7207.0
83	5631.5
84	4056.0
85	3182.0
86	2308.0
87	1780.0
88	1252.0
89	1252.0
90	966.5
91	681.0
92	416.5
93	152.0
94	98.5
95	45.0
96	45.0
97	29.0
98	13.0
99	9.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2976400.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.364689647915114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.98151898403076	53.37593917559285
2	7.767292659052296	9.53275006854547
3	2.0493976384194914	3.7728195014034682
4	0.8992606016406769	2.2073139092911025
5	0.4869783959230159	1.49416390655277
6	0.32912722832964575	1.2118074132676326
7	0.2261855268750879	0.9715863261677952
8	0.1687281466240935	0.8283160281964331
9	0.12759833575905405	0.7047029046100339
>10	0.7950403249017359	9.25552067173068
>50	0.08393297921593684	3.6225365682240955
>100	0.0781414823580983	9.531430191847887
>500	0.005810934421203361	2.409816181915207
>1k	9.867624488029336E-4	1.0812971526546347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4884	0.16409084800430052	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3882	0.13042601800833223	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3562	0.11967477489584734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.3597634726515254E-5	0.0	0.0	0.0
6	0.0	3.3597634726515254E-5	0.0	0.0	0.0
7	0.0	3.3597634726515254E-5	0.0	0.0	0.0
8	0.0	3.3597634726515254E-5	0.0	3.3597634726515254E-5	0.0
9	0.0	3.3597634726515254E-5	0.0	6.719526945303051E-5	0.0
10	3.3597634726515254E-5	3.3597634726515254E-5	0.0	6.719526945303051E-5	0.0
11	3.3597634726515254E-5	3.3597634726515254E-5	0.0	6.719526945303051E-5	0.0
12	3.3597634726515254E-5	6.719526945303051E-5	0.0	2.0158580835909153E-4	0.0
13	3.3597634726515254E-5	6.719526945303051E-5	0.0	2.0158580835909153E-4	0.0
14	3.3597634726515254E-5	6.719526945303051E-5	0.0	2.3518344308560678E-4	0.0
15	3.3597634726515254E-5	6.719526945303051E-5	0.0	2.3518344308560678E-4	0.0
16	3.3597634726515254E-5	6.719526945303051E-5	0.0	5.375621556242441E-4	0.0
17	3.3597634726515254E-5	6.719526945303051E-5	0.0	0.001209514850154549	0.0
18	3.3597634726515254E-5	6.719526945303051E-5	0.0	0.0013775030237871255	0.0
19	3.3597634726515254E-5	6.719526945303051E-5	0.0	0.001579088832146217	0.0
20	3.3597634726515254E-5	6.719526945303051E-5	0.0	0.001847869909958339	0.0
21	3.3597634726515254E-5	6.719526945303051E-5	0.0	0.0024526273350356136	0.0
22	3.3597634726515254E-5	6.719526945303051E-5	0.0	0.0035949469157371323	0.0
23	3.3597634726515254E-5	6.719526945303051E-5	0.0	0.004905254670071227	0.0
24	3.3597634726515254E-5	1.0079290417954576E-4	0.0	0.006954710388388657	0.0
25	3.3597634726515254E-5	1.0079290417954576E-4	0.0	0.00806343233436366	0.0
26	3.3597634726515254E-5	1.0079290417954576E-4	0.0	0.01112081709447655	0.0
27	3.3597634726515254E-5	1.0079290417954576E-4	0.0	0.024660663889262194	0.0
28	3.3597634726515254E-5	1.0079290417954576E-4	0.0	0.07458674909286386	0.0
29	3.3597634726515254E-5	1.0079290417954576E-4	0.0	0.14682166375487166	0.0
30	3.3597634726515254E-5	1.0079290417954576E-4	0.0	0.24435559736594545	0.0
31	3.3597634726515254E-5	1.0079290417954576E-4	0.0	0.5387716704743986	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1845	0.0	23.262873	1
AACGCTA	140	1.873559E-9	17.178572	33
TACGTTA	145	2.9849616E-9	16.586208	19
CTTATAC	1735	0.0	16.31412	37
TTAACGG	70	0.0025937923	15.857142	35
TCTTATA	2945	0.0	15.578948	37
GTATACG	100	1.0942912E-4	14.8	1
CTTAACG	165	1.6480044E-8	14.575757	30
TAACCCG	395	0.0	14.518987	28
TAGGACA	900	0.0	14.388889	4
GTATCAA	2965	0.0	14.288364	2
ACGTTAA	170	2.4410838E-8	14.1470585	20
TAACGCT	170	2.4410838E-8	14.1470585	32
CGACCAT	290	0.0	14.034484	10
GCTTAGG	835	0.0	13.958084	1
TTAGGAC	1020	0.0	13.784315	3
AATTACG	175	3.5719495E-8	13.742857	16
GACCATA	325	0.0	13.661539	11
TCTAGCG	220	2.8558134E-10	13.454545	28
CTTAGGA	985	0.0	13.335026	2
>>END_MODULE