FastQCFastQC Report
Fri 10 Feb 2017
ERR1632322.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632322.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73206
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27153.7087124006228995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24203.3057399666693987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17042.3276780591754775No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8321.1365188645739421No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.4671748217359233No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT2930.40024041745212147TruSeq Adapter, Index 11 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.37838428544108405No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT2040.2786656831407262No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.22265934486244296No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.21309728710761414No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.20626724585416498No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.15435893232795128No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.1434308663224326No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1020.1393328415703631No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1010.13796683331967324No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG900.12294074256208506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG860.11747670955932574No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.11747670955932574No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG820.1120126765565664No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC810.11064666830587658No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT810.11064666830587658No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC810.11064666830587658No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA780.10654864355380707No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.10381662705242739No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.10381662705242739No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC740.10108461055104773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT507.1340764E-933.30000336
GTGTATA250.005463202529.613
GAACGCC250.005463202529.69
GTATAAG250.005463202529.615
TCAGATG250.005463202529.67
GGAACGC250.005463202529.68
CCGTCTT604.2076863E-827.75000237
TCTCGTA556.1282117E-726.9090928
CGTATGC556.1282117E-726.9090931
CTCGTAT556.1282117E-726.9090929
TGCAATC358.7899755E-426.42857237
TAAGAGA358.7899755E-426.42857218
GGGAACG358.7899755E-426.4285727
CCATCGT508.940229E-625.90000223
GTATGCC601.3090594E-624.66666832
TATGCCG601.3090594E-624.66666833
TACGCCA451.3067527E-424.66666830
TCGTATG601.3090594E-624.66666830
GTGGCCA451.3067527E-424.66666819
TTACGCC451.3067527E-424.66666829
Produced by FastQC (version 0.11.5)