##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632322.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73206
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69344042838019	31.0	31.0	34.0	30.0	34.0
2	31.791178318717044	31.0	31.0	34.0	30.0	34.0
3	31.675040297243395	31.0	31.0	34.0	30.0	34.0
4	35.554667650192606	37.0	35.0	37.0	33.0	37.0
5	35.16273256290468	37.0	35.0	37.0	32.0	37.0
6	35.24116875665929	37.0	35.0	37.0	32.0	37.0
7	35.19665054776931	37.0	35.0	37.0	32.0	37.0
8	35.22230418271727	37.0	35.0	37.0	32.0	37.0
9	36.74556730322651	38.0	37.0	39.0	32.0	39.0
10	36.551635111876074	38.0	35.0	39.0	32.0	39.0
11	36.754869819413706	38.0	37.0	39.0	32.0	39.0
12	36.67096959265634	38.0	35.0	39.0	32.0	39.0
13	36.77908914569844	38.0	37.0	39.0	32.0	39.0
14	37.62004480507062	39.0	37.0	40.0	33.0	41.0
15	37.62223041827173	39.0	37.0	40.0	33.0	41.0
16	37.431344425320326	39.0	36.0	40.0	32.0	41.0
17	37.66274622298719	39.0	37.0	40.0	33.0	41.0
18	37.82374395541349	39.0	37.0	40.0	33.0	41.0
19	37.9265224161954	39.0	37.0	40.0	33.0	41.0
20	37.953596699724066	39.0	37.0	40.0	33.0	41.0
21	37.90705679862307	39.0	37.0	41.0	33.0	41.0
22	37.82542414556184	39.0	37.0	40.0	33.0	41.0
23	37.729694287353496	39.0	37.0	41.0	33.0	41.0
24	37.600032784198014	39.0	36.0	40.0	33.0	41.0
25	37.49129852744311	39.0	36.0	40.0	33.0	41.0
26	37.25780673715269	39.0	36.0	40.0	32.0	41.0
27	37.066265060240966	39.0	35.0	40.0	32.0	41.0
28	36.84631041171489	38.0	35.0	40.0	31.0	41.0
29	36.525708275277985	38.0	35.0	40.0	31.0	41.0
30	36.23054121246892	38.0	35.0	40.0	31.0	41.0
31	35.83602436958719	38.0	35.0	40.0	30.0	41.0
32	35.4869273010409	37.0	35.0	40.0	30.0	41.0
33	35.10441767068273	36.0	34.0	40.0	29.0	41.0
34	34.95139742644046	36.0	34.0	40.0	28.0	41.0
35	34.69707401032702	36.0	33.0	40.0	26.0	41.0
36	34.45072808239762	36.0	33.0	40.0	25.0	41.0
37	34.19884982105292	36.0	33.0	40.0	24.0	41.0
38	33.887413599978146	35.0	33.0	39.0	22.0	41.0
39	33.40586837144496	35.0	33.0	39.0	21.0	41.0
40	33.04500997186023	35.0	33.0	39.0	18.0	40.0
41	32.650274567658386	35.0	32.0	39.0	15.0	40.0
42	32.16836051689752	35.0	31.0	39.0	12.0	40.0
43	31.148444116602466	35.0	30.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	0.0
18	6.0
19	14.0
20	32.0
21	53.0
22	90.0
23	164.0
24	248.0
25	418.0
26	570.0
27	823.0
28	1207.0
29	1591.0
30	2018.0
31	2637.0
32	3216.0
33	4122.0
34	5524.0
35	7422.0
36	11179.0
37	14743.0
38	12201.0
39	4923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.99221375297107	18.94516842881731	16.741797120454606	19.320820697757014
2	18.999808758844903	24.71382127148048	32.56427068819496	23.72209928147966
3	21.953118596836322	25.86126820205994	32.30063109581182	19.884982105291915
4	14.185995683413926	19.345408846269432	29.552222495423873	36.91637297489277
5	12.483949403054394	40.20025680955113	30.590388765948145	16.72540502144633
6	28.527716307406497	33.44807802639128	17.78406141573095	20.240144250471275
7	24.248012457995248	35.44518208889982	19.93689041881813	20.369915034286805
8	32.79102805780947	30.388219544846052	18.53809797011174	18.282654427232742
9	27.369341310821515	12.214845777668497	21.132147638171734	39.28366527333825
10	21.201814058956916	29.90328661585116	25.75745157500751	23.13744775018441
11	31.653143184984838	24.857252137802913	17.94661639756304	25.542988279649208
12	20.35625495177991	33.7950441220665	26.59481463268038	19.25388629347321
13	33.79914214681857	19.838537824768462	27.03466928940251	19.327650739010462
14	22.586946425156405	23.96524875010245	29.850012294074258	23.597792530666887
15	28.18894626123542	27.134387891702865	25.592164576674044	19.084501270387673
16	20.4518755293282	26.164522033713084	29.93333879736634	23.450263639592382
17	17.63106849165369	31.62582301997104	29.863672376581153	20.879436111794114
18	17.70073491243887	23.890118296314512	34.648799278747646	23.760347512498974
19	18.920580280304893	27.714941398246047	36.90271289238587	16.461765429063192
20	20.731907220719613	23.88055623855968	37.54473677021009	17.842799770510613
21	23.086905444908886	23.705707182471382	35.52167855093845	17.685708821681285
22	21.532388055623855	25.668661038712674	34.710269650028685	18.088681255634782
23	19.1883178974401	26.53471026965003	35.16241838100702	19.11455345190285
24	17.972570554326147	27.44173974810808	36.43007403764719	18.155615659918585
25	17.844165778761305	27.728601480752946	35.1391962407453	19.288036499740457
26	19.012102833101114	26.99232303363112	35.36595361035981	18.62962052290796
27	18.07092314837582	27.697183290987077	34.87282463186078	19.35906892877633
28	17.351036800262275	27.67532715897604	36.38636177362511	18.587274267136575
29	17.264978280468814	28.376089391579924	36.53389066469961	17.825041663251646
30	17.107887331639482	28.871950386580338	37.04887577520968	16.9712865065705
31	17.1324754801519	29.5754446356856	35.669207442012954	17.62287244214955
32	16.700816872933913	30.128677977214984	36.39182580662787	16.77867934322323
33	16.617490369641832	29.011283228150695	36.55301478020927	17.8182116219982
34	17.106521323388794	29.74073163401907	35.016255498183206	18.136491544408926
35	16.509575717837336	30.06174357293118	35.47796628691637	17.950714422315112
36	16.7295030461984	29.76258776603011	34.098297953719644	19.409611234051855
37	16.47815752807147	30.057645548179114	34.36876758735623	19.09542933639319
38	16.632516460399422	28.82140808130481	35.04903969619976	19.497035762096004
39	17.11335136464224	28.287298855285087	35.514848509684995	19.084501270387673
40	16.55055596535803	28.15889407972024	35.43152200639292	19.85902794852881
41	15.911264104035189	28.44438980411442	35.30858126383084	20.335764828019563
42	16.087479168374177	27.840614157309513	35.311313280332215	20.7605933939841
43	15.692702783924814	28.68480725623583	35.11597410048356	20.50651585935579
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	13.0
2	20.0
3	90.0
4	160.0
5	160.0
6	256.5
7	353.0
8	399.0
9	445.0
10	669.5
11	894.0
12	894.0
13	1685.0
14	2476.0
15	3877.5
16	5279.0
17	4629.5
18	3980.0
19	3980.0
20	4295.0
21	4610.0
22	3212.0
23	1814.0
24	1551.5
25	1289.0
26	1289.0
27	1348.0
28	1407.0
29	1432.5
30	1458.0
31	1588.0
32	1718.0
33	1718.0
34	1594.5
35	1471.0
36	1476.5
37	1482.0
38	1678.5
39	1875.0
40	1875.0
41	2207.0
42	2539.0
43	2915.0
44	3291.0
45	3788.5
46	4286.0
47	4286.0
48	5037.0
49	5788.0
50	5600.0
51	5412.0
52	4745.0
53	4078.0
54	4078.0
55	3831.5
56	3585.0
57	3482.0
58	3379.0
59	2947.0
60	2515.0
61	2515.0
62	2209.0
63	1903.0
64	1756.0
65	1609.0
66	1447.0
67	1285.0
68	1285.0
69	1140.0
70	995.0
71	863.5
72	732.0
73	616.0
74	500.0
75	500.0
76	391.5
77	283.0
78	227.5
79	172.0
80	119.5
81	67.0
82	67.0
83	51.0
84	35.0
85	21.0
86	7.0
87	7.0
88	7.0
89	7.0
90	3.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	73206.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.97177826954074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.05276128598634	53.0776165888042
2	6.958664724991598	8.48564325328525
3	2.240394309398454	4.098024752069502
4	1.066427691273664	2.600879709313444
5	0.6765990814383331	2.0626724585416496
6	0.37414584966954184	1.3687402671912139
7	0.2800492886748068	1.1952572193536048
8	0.2038758821552593	0.9944540065021993
9	0.16802957320488407	0.9220555692156381
>10	0.8782345692841941	9.197333551894653
>50	0.06721182928195363	2.7770947736524327
>100	0.024644337403382996	2.741578559134497
>500	0.002240394309398454	1.1365188645739421
>1k	0.006721182928195362	9.342130426467776
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2715	3.7087124006228995	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2420	3.3057399666693987	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1704	2.3276780591754775	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	832	1.1365188645739421	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	342	0.4671748217359233	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	293	0.40024041745212147	TruSeq Adapter, Index 11 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	277	0.37838428544108405	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	204	0.2786656831407262	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	0.22265934486244296	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	156	0.21309728710761414	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	151	0.20626724585416498	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	113	0.15435893232795128	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105	0.1434308663224326	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	102	0.1393328415703631	No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	101	0.13796683331967324	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	90	0.12294074256208506	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	86	0.11747670955932574	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86	0.11747670955932574	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	82	0.1120126765565664	No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	81	0.11064666830587658	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	81	0.11064666830587658	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	81	0.11064666830587658	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	78	0.10654864355380707	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76	0.10381662705242739	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76	0.10381662705242739	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	74	0.10108461055104773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.001366008250689834	0.0
13	0.0	0.0	0.0	0.001366008250689834	0.0
14	0.0	0.0	0.0	0.001366008250689834	0.0
15	0.0	0.0	0.0	0.002732016501379668	0.0
16	0.0	0.0	0.0	0.004098024752069503	0.0
17	0.0	0.0	0.0	0.006830041253449171	0.0
18	0.0	0.0	0.0	0.006830041253449171	0.0
19	0.0	0.0	0.0	0.009562057754828839	0.0
20	0.0	0.0	0.0	0.010928066005518672	0.0
21	0.0	0.0	0.0	0.010928066005518672	0.0
22	0.0	0.0	0.0	0.017758107258967846	0.0
23	0.0	0.0	0.0	0.019124115509657678	0.0
24	0.0	0.0	0.0	0.03278419801655602	0.0
25	0.0	0.0	0.0	0.040980247520695026	0.0
26	0.0	0.0	0.0	0.04917629702483403	0.0
27	0.0	0.0	0.0	0.0683004125344917	0.0
28	0.0	0.0	0.0	0.12840477556484442	0.0
29	0.0	0.0	0.0	0.22675736961451248	0.0
30	0.0	0.0	0.0	0.4234625577138486	0.0
31	0.0	0.0	0.0	0.7717946616397563	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	50	7.1340764E-9	33.300003	36
GTGTATA	25	0.0054632025	29.6	13
GAACGCC	25	0.0054632025	29.6	9
GTATAAG	25	0.0054632025	29.6	15
TCAGATG	25	0.0054632025	29.6	7
GGAACGC	25	0.0054632025	29.6	8
CCGTCTT	60	4.2076863E-8	27.750002	37
TCTCGTA	55	6.1282117E-7	26.90909	28
CGTATGC	55	6.1282117E-7	26.90909	31
CTCGTAT	55	6.1282117E-7	26.90909	29
TGCAATC	35	8.7899755E-4	26.428572	37
TAAGAGA	35	8.7899755E-4	26.428572	18
GGGAACG	35	8.7899755E-4	26.428572	7
CCATCGT	50	8.940229E-6	25.900002	23
GTATGCC	60	1.3090594E-6	24.666668	32
TATGCCG	60	1.3090594E-6	24.666668	33
TACGCCA	45	1.3067527E-4	24.666668	30
TCGTATG	60	1.3090594E-6	24.666668	30
GTGGCCA	45	1.3067527E-4	24.666668	19
TTACGCC	45	1.3067527E-4	24.666668	29
>>END_MODULE