FastQCFastQC Report
Fri 10 Feb 2017
ERR1632321.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632321.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736268
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG303.5982326E-430.83333430
TAAGGCG250.00549507729.5999985
TCTATAG250.00549507729.5999983
ACATGTA1300.028.4615388
GGTATCA3150.025.2539671
GTTCTAG1850.025.01
ATTAGAC451.3225806E-424.6666683
TCTAGAC2100.024.6666663
CTAGACA2250.023.8444444
CTAGAAC1750.022.1999993
TTAGAAC502.7008247E-422.1999993
ACTGATC1850.021.08
AATCTAC1151.3460522E-1020.91304414
TAGACAT2400.020.81255
GAATCTA1251.8189894E-1120.72000113
ACTAGGC450.003824520420.5555573
CTCTAAC450.003824520420.5555571
TTCTAGA2450.020.3877542
CAATTAC1550.020.29032318
GACATGT1750.020.0857147
Produced by FastQC (version 0.11.5)