##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632321.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	736268
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10406265109987	33.0	31.0	34.0	30.0	34.0
2	32.29833837678672	34.0	31.0	34.0	30.0	34.0
3	32.31941222489637	34.0	31.0	34.0	30.0	34.0
4	35.99454410622219	37.0	35.0	37.0	35.0	37.0
5	35.69502001988406	37.0	35.0	37.0	33.0	37.0
6	35.67398555960601	37.0	35.0	37.0	33.0	37.0
7	35.67172687119364	37.0	35.0	37.0	33.0	37.0
8	35.629625353811385	37.0	35.0	37.0	33.0	37.0
9	37.3361927993611	39.0	37.0	39.0	34.0	39.0
10	37.17518892577159	39.0	37.0	39.0	34.0	39.0
11	37.30837684104158	39.0	37.0	39.0	34.0	39.0
12	37.2548569270972	39.0	37.0	39.0	34.0	39.0
13	37.3035171975422	39.0	37.0	39.0	34.0	39.0
14	38.51566684957108	40.0	38.0	41.0	35.0	41.0
15	38.496931823738095	40.0	38.0	41.0	35.0	41.0
16	38.39847039393264	40.0	38.0	41.0	34.0	41.0
17	38.444867901362	40.0	38.0	41.0	34.0	41.0
18	38.45472708307301	40.0	38.0	41.0	34.0	41.0
19	38.496338289861846	40.0	38.0	41.0	34.0	41.0
20	38.45878267152722	40.0	38.0	41.0	34.0	41.0
21	38.42088342831686	40.0	38.0	41.0	34.0	41.0
22	38.409648388901864	40.0	38.0	41.0	34.0	41.0
23	38.37194472664845	40.0	38.0	41.0	34.0	41.0
24	38.3081731108781	40.0	38.0	41.0	34.0	41.0
25	38.22536359043175	40.0	38.0	41.0	34.0	41.0
26	38.13207283217524	40.0	38.0	41.0	34.0	41.0
27	38.04741480004564	40.0	38.0	41.0	34.0	41.0
28	37.97317417027495	40.0	38.0	41.0	33.0	41.0
29	37.83596869618128	40.0	37.0	41.0	33.0	41.0
30	37.774008920664755	40.0	37.0	41.0	33.0	41.0
31	37.695889268581546	39.0	37.0	41.0	33.0	41.0
32	37.608635714169296	39.0	37.0	40.0	33.0	41.0
33	37.46978002575149	39.0	37.0	40.0	32.0	41.0
34	37.46335980919991	39.0	37.0	40.0	32.0	41.0
35	37.44434635214351	39.0	37.0	40.0	32.0	41.0
36	37.42411323050845	39.0	37.0	40.0	32.0	41.0
37	37.41325848739861	39.0	37.0	40.0	32.0	41.0
38	37.359089353333296	39.0	36.0	40.0	32.0	41.0
39	37.176060890871256	39.0	36.0	40.0	31.0	41.0
40	37.187969598026804	39.0	36.0	40.0	31.0	41.0
41	37.20069322583625	39.0	36.0	40.0	32.0	41.0
42	37.096391531344565	39.0	36.0	40.0	31.0	41.0
43	36.180328358695476	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	1.0
16	5.0
17	11.0
18	13.0
19	42.0
20	83.0
21	143.0
22	296.0
23	495.0
24	913.0
25	1334.0
26	2009.0
27	3120.0
28	4371.0
29	6323.0
30	8849.0
31	11952.0
32	16270.0
33	22399.0
34	32076.0
35	48008.0
36	76300.0
37	131456.0
38	224653.0
39	145144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6126519147919	17.254858285298287	11.476663388874703	27.65582641103511
2	20.341098621697533	19.969902263849576	32.092661911151914	27.596337203300976
3	21.262230600813833	20.20541433282446	28.50864087533344	30.023714191028265
4	16.30466080285983	14.428577637490697	31.685201584205753	37.581559975443724
5	17.290986434287515	33.75224782280365	32.48382382502024	16.472941917888594
6	38.52754703450374	32.01456534848723	14.168210488572097	15.289677128436926
7	32.61910065356636	27.76258101669501	19.29745690427942	20.320861425459206
8	29.473914389868906	31.097236332422433	18.881032450140438	20.547816827568223
9	28.09574774402799	12.787870720987465	17.589383213721092	41.526998321263456
10	17.95419602644689	25.498459799964145	31.507413061548238	25.039931112040726
11	39.592512509032034	19.3702564826938	19.248290024827917	21.788940983446246
12	24.50507152286939	23.084800643244037	26.88273835070925	25.52738948317732
13	33.28760722997604	17.652539564397745	22.304242476924163	26.755610728702052
14	25.14043799268745	17.90394258612353	22.365089885748123	34.5905295354409
15	27.575420906517735	25.1824064063629	20.16792798274541	27.07424470437395
16	27.76597651941956	23.269380171350647	21.547045369349206	27.41759793988059
17	26.190327435118732	23.985287965795063	22.849560214487116	26.97482438459909
18	26.942906658988303	22.64433602981523	23.563566527405783	26.849190783790682
19	27.33067307013207	22.802702276888308	23.057636621447624	26.808988031531992
20	27.481433391102154	23.10340799817458	23.36187366556743	26.05328494515584
21	28.163657798519015	22.719172909864344	22.968402809846413	26.148766481770224
22	28.26497959981963	22.61445560583918	22.152531415191206	26.968033379149986
23	27.317091059233867	22.984972863142225	22.66294338474577	27.034992692878134
24	27.241710898748828	22.562979784534978	22.3690286689086	27.826280647807593
25	27.548121064612342	22.50892338116012	22.887725665111073	27.05522988911646
26	27.637626516431517	22.07606469383431	22.493168248518202	27.793140541215973
27	27.076282006008682	22.593403488946958	23.066736568749423	27.263577936294936
28	26.71079009273797	22.644607670033196	23.23746244573987	27.407139791488973
29	26.852043006079306	22.897640533066763	22.863413865603285	27.386902595250646
30	26.444175218806194	23.082084241064397	23.47745657831116	26.99628396181825
31	27.45780069213928	22.948708894044017	22.63577936294936	26.957711050867346
32	26.487909293898415	22.79631873176615	23.279023399088373	27.436748575247055
33	26.579180407134356	22.62518539444876	23.436438905398578	27.359195293018303
34	26.927558986673333	21.921365589703747	23.699794096714783	27.451281326908138
35	26.102451824607343	22.25589051812655	24.338827709475357	27.30282994779075
36	26.29613130001576	21.658553678823473	24.74153433260715	27.303780688553626
37	26.234061510210953	21.475739812133625	24.705134543399957	27.58506413425546
38	25.50837466791983	21.092319644477282	25.921267799225284	27.4780378883776
39	25.526846202741392	21.210754779509635	26.399218762733135	26.86318025501584
40	25.016570053295812	21.297951289476114	26.64152183715712	27.043956820070953
41	24.89501105575687	20.32968973254304	27.713006676916557	27.062292534783527
42	23.941553890702842	20.280115392764593	28.183351714321415	27.59497900221115
43	22.78504566272064	20.096079145093906	28.664290720226877	28.454584471958583
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	8.0
2	9.0
3	16.0
4	23.0
5	23.0
6	32.5
7	42.0
8	48.5
9	55.0
10	68.0
11	81.0
12	81.0
13	141.5
14	202.0
15	341.5
16	481.0
17	480.5
18	480.0
19	480.0
20	645.0
21	810.0
22	954.5
23	1099.0
24	1550.5
25	2002.0
26	2002.0
27	2589.5
28	3177.0
29	4166.5
30	5156.0
31	6583.5
32	8011.0
33	8011.0
34	10329.5
35	12648.0
36	15498.0
37	18348.0
38	20972.0
39	23596.0
40	23596.0
41	26726.0
42	29856.0
43	34196.5
44	38537.0
45	43853.0
46	49169.0
47	49169.0
48	52036.5
49	54904.0
50	58523.5
51	62143.0
52	64073.5
53	66004.0
54	66004.0
55	66349.0
56	66694.0
57	64739.5
58	62785.0
59	61975.5
60	61166.0
61	61166.0
62	56688.5
63	52211.0
64	45549.5
65	38888.0
66	34377.0
67	29866.0
68	29866.0
69	24593.5
70	19321.0
71	15828.0
72	12335.0
73	9729.5
74	7124.0
75	7124.0
76	5518.5
77	3913.0
78	3101.0
79	2289.0
80	1843.0
81	1397.0
82	1397.0
83	1060.0
84	723.0
85	565.0
86	407.0
87	303.0
88	199.0
89	199.0
90	141.5
91	84.0
92	52.5
93	21.0
94	12.0
95	3.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	736268.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.95942841373311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.47080924438987	55.07112152913469
2	6.73248601218222	8.477469422608921
3	1.9642629705404437	3.7100662163846345
4	0.9769336994206438	2.460287492545499
5	0.5553600167120557	1.7482574608016153
6	0.3717748163662303	1.4044037962223068
7	0.2712541592402748	1.195460477443081
8	0.21722177696570094	1.094092713342076
9	0.16592099048698675	0.9401661650611021
>10	1.1045687171672451	13.404065713480684
>50	0.1178490432154857	5.097222525229145
>100	0.05112528815908831	5.251994073847781
>500	4.3326515380237373E-4	0.14539241389847332
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3582010898205546E-4
2	0.0	0.0	0.0	0.0	1.3582010898205546E-4
3	0.0	0.0	0.0	0.0	1.3582010898205546E-4
4	0.0	0.0	0.0	0.0	1.3582010898205546E-4
5	0.0	0.0	0.0	0.0	1.3582010898205546E-4
6	0.0	0.0	0.0	0.0	1.3582010898205546E-4
7	0.0	0.0	0.0	0.0	1.3582010898205546E-4
8	0.0	0.0	0.0	0.0	1.3582010898205546E-4
9	0.0	0.0	0.0	1.3582010898205546E-4	1.3582010898205546E-4
10	0.0	0.0	0.0	2.716402179641109E-4	1.3582010898205546E-4
11	0.0	0.0	0.0	2.716402179641109E-4	1.3582010898205546E-4
12	0.0	0.0	0.0	2.716402179641109E-4	1.3582010898205546E-4
13	0.0	0.0	0.0	2.716402179641109E-4	1.3582010898205546E-4
14	0.0	0.0	0.0	2.716402179641109E-4	1.3582010898205546E-4
15	0.0	0.0	0.0	2.716402179641109E-4	1.3582010898205546E-4
16	0.0	0.0	0.0	4.074603269461663E-4	1.3582010898205546E-4
17	0.0	0.0	0.0	6.791005449102772E-4	1.3582010898205546E-4
18	0.0	0.0	0.0	8.149206538923327E-4	1.3582010898205546E-4
19	0.0	0.0	0.0	8.149206538923327E-4	1.3582010898205546E-4
20	0.0	0.0	0.0	8.149206538923327E-4	1.3582010898205546E-4
21	0.0	0.0	0.0	8.149206538923327E-4	2.716402179641109E-4
22	0.0	0.0	0.0	0.001222380980838499	2.716402179641109E-4
23	0.0	1.3582010898205546E-4	0.0	0.00298804239760522	2.716402179641109E-4
24	0.0	1.3582010898205546E-4	0.0	0.0040746032694616635	2.716402179641109E-4
25	0.0	1.3582010898205546E-4	0.0	0.0050253440323360515	2.716402179641109E-4
26	0.0	1.3582010898205546E-4	0.0	0.006519365231138661	2.716402179641109E-4
27	0.0	1.3582010898205546E-4	0.0	0.023089418526949426	2.716402179641109E-4
28	0.0	4.074603269461663E-4	0.0	0.08733233007546165	2.716402179641109E-4
29	0.0	4.074603269461663E-4	0.0	0.1847153482155954	2.716402179641109E-4
30	0.0	4.074603269461663E-4	0.0	0.30858328760723	2.716402179641109E-4
31	0.0	4.074603269461663E-4	0.0	0.6656543541210538	2.716402179641109E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	30	3.5982326E-4	30.833334	30
TAAGGCG	25	0.005495077	29.599998	5
TCTATAG	25	0.005495077	29.599998	3
ACATGTA	130	0.0	28.461538	8
GGTATCA	315	0.0	25.253967	1
GTTCTAG	185	0.0	25.0	1
ATTAGAC	45	1.3225806E-4	24.666668	3
TCTAGAC	210	0.0	24.666666	3
CTAGACA	225	0.0	23.844444	4
CTAGAAC	175	0.0	22.199999	3
TTAGAAC	50	2.7008247E-4	22.199999	3
ACTGATC	185	0.0	21.0	8
AATCTAC	115	1.3460522E-10	20.913044	14
TAGACAT	240	0.0	20.8125	5
GAATCTA	125	1.8189894E-11	20.720001	13
ACTAGGC	45	0.0038245204	20.555557	3
CTCTAAC	45	0.0038245204	20.555557	1
TTCTAGA	245	0.0	20.387754	2
CAATTAC	155	0.0	20.290323	18
GACATGT	175	0.0	20.085714	7
>>END_MODULE