FastQCFastQC Report
Fri 10 Feb 2017
ERR1632320.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632320.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1223258
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6400.026.8828121
TATGCCG656.902595E-519.92307733
GTACCGT609.237278E-418.56
CTCGTAT701.2192788E-418.529
TCGTATT1103.8522558E-818.526
CGTGTTA500.007035133418.515
TAGTACC609.237278E-418.54
TATACAG953.6067704E-617.5263165
GTATAGA1401.869921E-917.1785721
GTTCCAA1554.0017767E-1016.7096791
CTTATAC9100.016.6703337
CGAACGA904.4463304E-516.44444516
ATAACGA904.4463304E-516.44444512
CGTATTG1151.2422788E-616.08695627
TATGGAC700.002592621615.8571423
ATCGTAC700.002592621615.85714225
CGTACTA700.002592621615.85714227
GTATCAA10850.015.6866361
CTATACA957.0602684E-515.5789484
ATAGACG855.3645007E-415.2352943
Produced by FastQC (version 0.11.5)