##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632320.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1223258
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.790934537113184	31.0	31.0	34.0	30.0	34.0
2	31.964216052541655	33.0	31.0	34.0	30.0	34.0
3	32.00501284275271	33.0	31.0	34.0	30.0	34.0
4	35.7694182257545	37.0	35.0	37.0	35.0	37.0
5	35.29556806495441	37.0	35.0	37.0	32.0	37.0
6	35.27069596111368	37.0	35.0	37.0	32.0	37.0
7	35.26515910789057	37.0	35.0	37.0	32.0	37.0
8	35.2056320089466	37.0	35.0	37.0	32.0	37.0
9	36.8777436975683	39.0	37.0	39.0	33.0	39.0
10	36.63557892120877	38.0	35.0	39.0	32.0	39.0
11	36.818962148622774	38.0	37.0	39.0	33.0	39.0
12	36.7142221837094	38.0	37.0	39.0	32.0	39.0
13	36.80801024804252	38.0	37.0	39.0	33.0	39.0
14	37.913852188172896	39.0	37.0	40.0	33.0	41.0
15	37.887070429950185	39.0	37.0	40.0	33.0	41.0
16	37.793158924773024	39.0	37.0	40.0	33.0	41.0
17	37.80753446942509	39.0	37.0	40.0	33.0	41.0
18	37.80920051207513	39.0	37.0	40.0	33.0	41.0
19	37.86000336805482	39.0	37.0	40.0	33.0	41.0
20	37.824907746362584	39.0	37.0	40.0	33.0	41.0
21	37.789098456744206	39.0	37.0	40.0	33.0	41.0
22	37.762717268147846	39.0	37.0	40.0	33.0	41.0
23	37.69426237147029	39.0	37.0	40.0	33.0	41.0
24	37.62125651334387	39.0	37.0	40.0	33.0	41.0
25	37.53406476802114	39.0	37.0	40.0	32.0	41.0
26	37.40028187021871	39.0	37.0	40.0	32.0	41.0
27	37.30491441707309	39.0	36.0	40.0	32.0	41.0
28	37.205272313771914	39.0	36.0	40.0	32.0	41.0
29	37.04781493356267	39.0	36.0	40.0	31.0	41.0
30	36.97168790230679	39.0	36.0	40.0	31.0	41.0
31	36.8757261346339	39.0	36.0	40.0	31.0	41.0
32	36.770564345379306	39.0	36.0	40.0	30.0	41.0
33	36.61691401159853	38.0	35.0	40.0	30.0	41.0
34	36.59913280763339	38.0	35.0	40.0	30.0	41.0
35	36.55812020031751	38.0	35.0	40.0	30.0	41.0
36	36.53323746911935	38.0	35.0	40.0	30.0	41.0
37	36.50946325305046	38.0	35.0	40.0	30.0	41.0
38	36.427783018790805	38.0	35.0	40.0	30.0	41.0
39	36.26044710110214	38.0	35.0	40.0	30.0	41.0
40	36.24447500036787	38.0	35.0	40.0	30.0	41.0
41	36.23289526820998	38.0	35.0	40.0	30.0	41.0
42	36.114432932382215	38.0	35.0	40.0	30.0	41.0
43	35.16146062400573	38.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	5.0
14	2.0
15	13.0
16	19.0
17	45.0
18	72.0
19	131.0
20	270.0
21	547.0
22	924.0
23	1592.0
24	2497.0
25	3710.0
26	5581.0
27	8029.0
28	11407.0
29	15614.0
30	21415.0
31	28387.0
32	38493.0
33	52627.0
34	74210.0
35	107122.0
36	161013.0
37	240460.0
38	306542.0
39	142530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.154428583340554	17.872108745661176	12.019214262240672	26.954248408757596
2	19.434739032975873	20.009760819058613	33.018218560598015	27.537281587367506
3	20.290486553122893	21.27457985151129	29.30052368347479	29.13440991189103
4	15.365523871497263	15.211427188704263	33.69550822475716	35.72754071504131
5	16.256995662403188	34.804023354026704	33.221528083200766	15.717452900369342
6	36.3900338276962	34.08357026890484	14.936587375680354	14.589808527718601
7	31.396483816169606	29.584928118189296	19.79958438857543	19.219003677065672
8	29.251310843665035	32.214545091877596	18.981277866157427	19.552866198299952
9	28.02360581332801	13.474099494955274	17.86622282462081	40.6360718670959
10	17.390852951707654	26.017160729788806	32.08677155595957	24.50521476254396
11	38.88558259990943	19.961283719378905	20.075977430762766	21.077156249948906
12	23.609083284147744	23.949076973132406	27.931229552555553	24.5106101901643
13	32.68501003059044	17.958108591973236	22.76159240323791	26.59528897419841
14	24.019381030003483	18.4925829220001	23.628294276432282	33.85974177156413
15	26.684395278837336	25.729976832360794	20.794959035624537	26.790668853177337
16	26.92252983426227	24.41872442281187	22.129755129334942	26.52899061359092
17	25.650843893929164	24.557125316163884	23.693529901296372	26.09850088861058
18	26.075856442385824	23.615296200801467	24.356677005177975	25.952170351634734
19	26.740638524334198	23.68461927083248	24.07693225795376	25.497809946879563
20	27.16123663201058	23.368496261622653	23.701459544920205	25.76880756144656
21	27.62197345122615	23.42253228672937	23.05237325241282	25.903121009631658
22	27.671922031165952	23.466513196725465	23.024088131857713	25.837476640250873
23	27.00869317838101	23.39179469907411	23.17932929929745	26.420182823247423
24	26.530789089464363	23.466186201112112	23.30734808192548	26.695676627498045
25	26.804566166744877	22.860835571890803	23.555374254654375	26.77922400670995
26	26.427867220161243	23.241131470221326	23.590117538573217	26.74088377104421
27	26.405059276129812	23.377815636603234	23.621182121841837	26.595942965425117
28	26.420019325440748	23.36694303245922	23.808877603906943	26.404160038193087
29	26.19210338293312	23.682820794959035	23.87288699522096	26.252188826886886
30	26.168314452061626	23.935179659564866	23.5041994411645	26.392306447209013
31	26.482230240881314	23.70759071267059	23.316994452519417	26.49318459392867
32	25.8645355272559	23.690423442969514	23.71756407887788	26.727476950896705
33	25.817448158932947	23.458174808584943	24.286781692823592	26.43759533965852
34	26.27556901324169	22.796417436060096	24.336076281536684	26.591937269161537
35	25.9091704284787	23.126029014320775	24.691030019832283	26.27377053736824
36	25.83208121263053	23.233201826597497	24.33893749315353	26.595779467618442
37	25.720984452993562	22.52836278201328	24.758963358506545	26.991689406486614
38	25.19190555058704	22.697501263020555	25.431184590658717	26.67940859573369
39	25.161249711835115	22.188532590835294	25.80518582343218	26.845031873897412
40	24.8496228923089	22.032637432168848	26.690444697684377	26.427294977837875
41	24.420441149781976	21.669263556829385	27.209386736076937	26.700908557311703
42	23.930928716591268	21.95710144548411	27.486188522780967	26.62578131514366
43	23.436838344813605	21.2104069623906	27.942837896829616	27.409916795966183
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	15.0
2	19.0
3	54.0
4	89.0
5	89.0
6	117.5
7	146.0
8	150.5
9	155.0
10	216.0
11	277.0
12	277.0
13	487.0
14	697.0
15	1252.0
16	1807.0
17	1899.0
18	1991.0
19	1991.0
20	2559.5
21	3128.0
22	3557.5
23	3987.0
24	5320.5
25	6654.0
26	6654.0
27	8580.0
28	10506.0
29	13145.0
30	15784.0
31	19064.0
32	22344.0
33	22344.0
34	26940.5
35	31537.0
36	36402.0
37	41267.0
38	46230.5
39	51194.0
40	51194.0
41	56380.0
42	61566.0
43	65658.0
44	69750.0
45	74187.0
46	78624.0
47	78624.0
48	82488.0
49	86352.0
50	89218.5
51	92085.0
52	94414.0
53	96743.0
54	96743.0
55	97331.5
56	97920.0
57	96151.5
58	94383.0
59	91024.0
60	87665.0
61	87665.0
62	81121.5
63	74578.0
64	66844.0
65	59110.0
66	52069.5
67	45029.0
68	45029.0
69	38360.5
70	31692.0
71	27031.0
72	22370.0
73	18031.5
74	13693.0
75	13693.0
76	10961.0
77	8229.0
78	6727.0
79	5225.0
80	4248.5
81	3272.0
82	3272.0
83	2507.0
84	1742.0
85	1330.5
86	919.0
87	678.0
88	437.0
89	437.0
90	314.5
91	192.0
92	127.0
93	62.0
94	40.5
95	19.0
96	19.0
97	11.5
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1223258.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.91729468874763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.1664307904987	65.17024046396364
2	7.308200549807503	10.804048273691771
3	1.86588918539172	4.137644423194411
4	0.7988259228529044	2.3618820457811567
5	0.442328946155614	1.634787953117385
6	0.2780286016628134	1.2330673248606363
7	0.2000803243378793	1.035257740685226
8	0.15624231089871912	0.9239207150041238
9	0.11898588543889466	0.7915603282009507
>10	0.6135743802060472	8.312053129173533
>50	0.0383384406656306	1.9057413466695368
>100	0.012742258565159866	1.504453773470519
>500	3.324035184289245E-4	0.18534248218717103
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.174890333846171E-5	0.0
8	0.0	0.0	0.0	8.174890333846171E-5	0.0
9	0.0	0.0	0.0	1.6349780667692342E-4	0.0
10	0.0	0.0	0.0	2.4524671001538515E-4	0.0
11	0.0	0.0	0.0	4.0874451669230857E-4	0.0
12	0.0	0.0	0.0	5.72242323369232E-4	0.0
13	0.0	0.0	0.0	6.539912267076937E-4	0.0
14	0.0	0.0	0.0	6.539912267076937E-4	0.0
15	0.0	0.0	0.0	8.174890333846171E-4	0.0
16	0.0	0.0	0.0	9.809868400615406E-4	0.0
17	0.0	0.0	0.0	0.001144484646738464	0.0
18	0.0	0.0	0.0	0.0013079824534153873	0.0
19	0.0	0.0	0.0	0.001389731356753849	0.0
20	0.0	0.0	0.0	0.0016349780667692343	0.0
21	0.0	0.0	0.0	0.001716726970107696	0.0
22	0.0	0.0	0.0	0.0022072203901384664	0.0
23	0.0	0.0	0.0	0.002534216003492313	0.0
24	0.0	0.0	0.0	0.0035152028435538538	0.0
25	0.0	0.0	0.0	0.004169194070261548	0.0
26	0.0	0.0	0.0	0.005640674330353858	0.0
27	0.0	0.0	0.0	0.017003771894400038	0.0
28	0.0	0.0	0.0	0.06163867311720013	0.0
29	0.0	0.0	0.0	0.13553968173516953	0.0
30	0.0	0.0	0.0	0.23625433064815435	0.0
31	0.0	0.0	0.0	0.5890008485536167	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	640	0.0	26.882812	1
TATGCCG	65	6.902595E-5	19.923077	33
GTACCGT	60	9.237278E-4	18.5	6
CTCGTAT	70	1.2192788E-4	18.5	29
TCGTATT	110	3.8522558E-8	18.5	26
CGTGTTA	50	0.0070351334	18.5	15
TAGTACC	60	9.237278E-4	18.5	4
TATACAG	95	3.6067704E-6	17.526316	5
GTATAGA	140	1.869921E-9	17.178572	1
GTTCCAA	155	4.0017767E-10	16.709679	1
CTTATAC	910	0.0	16.67033	37
CGAACGA	90	4.4463304E-5	16.444445	16
ATAACGA	90	4.4463304E-5	16.444445	12
CGTATTG	115	1.2422788E-6	16.086956	27
TATGGAC	70	0.0025926216	15.857142	3
ATCGTAC	70	0.0025926216	15.857142	25
CGTACTA	70	0.0025926216	15.857142	27
GTATCAA	1085	0.0	15.686636	1
CTATACA	95	7.0602684E-5	15.578948	4
ATAGACG	85	5.3645007E-4	15.235294	3
>>END_MODULE