FastQCFastQC Report
Fri 10 Feb 2017
ERR1632319.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632319.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319991
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43050.32613858730854983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38460.2913656229474292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30970.23462281182220182No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.11181894422007423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10500.029.5999981
CGTATTA656.9031215E-519.92307715
CTTATAC13050.019.5632237
GCCGTCT2250.018.91111236
AGAGCGT701.21937126E-418.58
TATGCCG2400.017.72916633
GTATCAA18000.017.1638892
CGTATGC2850.014.92982631
AAACCGT750.00410483514.86
CCGTCTT2900.014.67241437
TTTAGCG908.27516E-414.38888826
GCATTAG908.27516E-414.3888881
ATCCGTA1051.6559243E-414.09523912
TCTTATA19300.013.99481937
TCGTATG3050.013.95081930
ATGCCGT3050.013.95081934
CTCGTAT3200.013.87500129
GTCTTAC1203.301089E-513.8749991
CTACTAG950.001245245213.63157751
CCGTATT950.001245245213.631577514
Produced by FastQC (version 0.11.5)