FastQCFastQC Report
Fri 10 Feb 2017
ERR1632318.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632318.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5256571
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62500.11889880304099384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54720.10409828003845092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24550.027.128311
CTTATAC34150.018.90629637
GTATCAA36800.017.595112
TCTAGAC12200.016.6803263
TAGAACT12350.015.72874454
GTCTAGA11350.015.4845811
TCTTATA57400.015.27700437
GTTCTAG12600.015.1230151
CTAGAAC13250.014.3811333
CTAGACA14600.014.3184934
ACTGATC12300.014.1382118
CAACGGA10300.013.83009724
GCAATTA10850.013.8110617
ACGGAGT9900.013.64141526
CTCTAGT12000.013.56666627
TAGACAT14200.013.5492965
CTCTTAT89750.013.29526537
GCCGTAT5150.013.2912638
AGCGTCA5900.013.1694923
TTCTAGA16900.013.1360952
Produced by FastQC (version 0.11.5)