##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632318.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5256571
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.47697557970776	31.0	30.0	31.0	27.0	34.0
2	30.644040953693956	31.0	30.0	33.0	27.0	34.0
3	30.689467145026672	31.0	31.0	34.0	27.0	34.0
4	34.541082390021934	35.0	35.0	37.0	30.0	37.0
5	33.069041015521336	35.0	32.0	37.0	26.0	37.0
6	32.99101733810882	35.0	32.0	37.0	26.0	37.0
7	33.02437882033744	35.0	32.0	37.0	26.0	37.0
8	32.93228018797806	35.0	32.0	37.0	26.0	37.0
9	34.311839029664014	37.0	32.0	39.0	27.0	39.0
10	33.82126142688836	35.0	32.0	38.0	27.0	39.0
11	34.13176441448237	37.0	32.0	39.0	27.0	39.0
12	33.99425880483684	35.0	32.0	38.0	27.0	39.0
13	34.17991443471419	37.0	32.0	39.0	27.0	39.0
14	34.93186908347666	37.0	33.0	39.0	27.0	40.0
15	34.87531396417931	37.0	33.0	39.0	27.0	40.0
16	34.76277310817261	37.0	33.0	39.0	27.0	40.0
17	34.74135743624503	37.0	33.0	39.0	27.0	40.0
18	34.744233645850116	37.0	33.0	39.0	27.0	40.0
19	34.81484013057181	37.0	33.0	39.0	27.0	40.0
20	34.74618548860084	37.0	33.0	39.0	27.0	40.0
21	34.684626156481094	37.0	32.0	39.0	27.0	40.0
22	34.64292444637388	37.0	32.0	39.0	26.0	40.0
23	34.56826703187306	37.0	32.0	39.0	26.0	40.0
24	34.434095915379054	37.0	32.0	39.0	25.0	40.0
25	34.29708226142099	37.0	32.0	39.0	25.0	40.0
26	34.081858686965326	37.0	32.0	39.0	25.0	40.0
27	33.92335649989318	36.0	32.0	39.0	24.0	40.0
28	33.77975128653261	36.0	31.0	39.0	24.0	40.0
29	33.55704640915152	36.0	31.0	39.0	24.0	40.0
30	33.43139472481205	36.0	31.0	39.0	24.0	40.0
31	33.263501434680514	36.0	31.0	39.0	23.0	40.0
32	33.11129080155105	36.0	30.0	38.0	23.0	40.0
33	32.90049311613978	36.0	30.0	38.0	21.0	40.0
34	32.84859083992207	36.0	30.0	38.0	21.0	40.0
35	32.78699136756642	35.0	30.0	38.0	21.0	40.0
36	32.7244551628809	35.0	30.0	38.0	21.0	40.0
37	32.69106114993976	35.0	30.0	38.0	21.0	40.0
38	32.57142802789119	35.0	30.0	38.0	21.0	40.0
39	32.35572562417591	35.0	30.0	38.0	19.0	40.0
40	32.30364071939673	35.0	30.0	38.0	19.0	40.0
41	32.260172838909625	35.0	30.0	38.0	19.0	40.0
42	32.09985178550808	35.0	30.0	38.0	19.0	40.0
43	31.130930410718317	34.0	27.0	38.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	11.0
11	25.0
12	45.0
13	131.0
14	278.0
15	598.0
16	1192.0
17	2341.0
18	4195.0
19	7355.0
20	12421.0
21	19458.0
22	30088.0
23	44478.0
24	63463.0
25	87950.0
26	118025.0
27	155379.0
28	198925.0
29	247693.0
30	301310.0
31	359484.0
32	421052.0
33	481164.0
34	536570.0
35	575131.0
36	574699.0
37	512192.0
38	366765.0
39	134147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.678976656074845	19.802890515509063	12.490842414189784	26.027290414226307
2	20.59625942463252	21.585820870677864	32.35261161696475	25.46530808772487
3	21.674528889650688	22.462247727653637	28.71185417261557	27.151369210080105
4	16.939864409707393	16.044128387117762	32.896901801573684	34.11910540160116
5	16.520465527812714	34.84891576657102	32.63950967275054	15.991109032865722
6	36.14380933882563	34.034753834771756	14.755493647855229	15.065943178547384
7	30.78598196428813	29.302866830867497	20.19272639901563	19.718424805828743
8	27.99248787850483	32.67886993250924	19.2624621640229	20.066180024963042
9	27.425996909392076	13.816155056214402	18.798699760737563	39.95914827365596
10	17.49499816515367	26.51317750678151	32.2299651236519	23.761859204412914
11	37.79943236760238	20.760149534744226	20.38773945981135	21.052678637842046
12	23.176154188728738	24.34370238697432	28.046020875586002	24.43412254871094
13	32.03616958659932	19.064880889081497	23.10510026403144	25.793849260287743
14	24.078434401437743	19.46613105768	23.885114459597332	32.57032008128493
15	26.432497534990013	26.561478956528884	21.026787234491838	25.979236273989258
16	26.48150286565139	24.958342615366558	22.60290976760325	25.9572447513788
17	25.014025302806715	25.311310358026173	24.216318965348325	25.458345373818787
18	25.54018579792797	24.225260155337004	24.791827219683707	25.44272682705132
19	25.91035106345943	24.387457146493407	24.685198773116543	25.016993016930616
20	26.181402286775924	24.495246045378252	24.505956449556184	24.817395218289644
21	26.571713765494653	24.18536722894069	24.203801299364166	25.039117706200486
22	26.547934004886454	24.2415445354015	23.81691410617302	25.39360735353903
23	26.091172363124173	24.316270055136705	24.038731713126293	25.55382586861283
24	25.98344053566479	24.163432777755688	23.78328001276878	26.069846673810744
25	26.314017255735727	24.027735951821064	24.038465379807484	25.619781412635728
26	26.14753990766985	23.816153153833554	24.128048493970688	25.908258444525913
27	25.801230497980526	24.084978591557118	24.214739989243938	25.899050921218414
28	25.645387458858636	24.075942282526004	24.60012430156465	25.67854595705071
29	25.700099932065978	24.281608676074192	24.351368981794405	25.666922410065425
30	25.53295675070307	24.558119732426327	24.23119558358481	25.677727933285787
31	26.13568807498272	24.348039815309257	24.078491472863202	25.43778063684482
32	25.509405275796716	24.208956751464026	24.3621364573978	25.919501515341466
33	25.40774204324454	24.038998046445105	24.71013898604242	25.843120924267932
34	25.9865414164481	23.387851129567164	24.73142662773888	25.894180826245854
35	25.342262094433803	23.77338763235577	25.208429601730863	25.675920671479563
36	25.688799789824962	23.277551087962095	25.226540267410062	25.807108854802873
37	25.443050231795596	22.91094327461762	25.526051108222454	26.119955385364335
38	24.8485942642076	23.03923983905097	26.282913328860204	25.829252567881227
39	24.83912040758129	22.806578661260353	26.526703434615456	25.827597496542896
40	24.39717831263004	23.004882840924246	26.92314057966686	25.674798266778858
41	24.31899045975028	22.444783871462974	27.366851888807357	25.869373779979384
42	23.938114790040885	22.610633433848797	27.364055388959834	26.087196387150485
43	23.375314439774524	22.24727869175552	27.692006823459632	26.685400045010333
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	175.0
1	216.0
2	257.0
3	567.0
4	877.0
5	877.0
6	1215.5
7	1554.0
8	1531.0
9	1508.0
10	2155.0
11	2802.0
12	2802.0
13	4842.0
14	6882.0
15	10357.5
16	13833.0
17	14676.5
18	15520.0
19	15520.0
20	18905.0
21	22290.0
22	24044.0
23	25798.0
24	31661.0
25	37524.0
26	37524.0
27	46266.5
28	55009.0
29	66480.5
30	77952.0
31	91433.0
32	104914.0
33	104914.0
34	125630.5
35	146347.0
36	169752.0
37	193157.0
38	211194.5
39	229232.0
40	229232.0
41	251446.0
42	273660.0
43	294878.0
44	316096.0
45	337322.0
46	358548.0
47	358548.0
48	371462.5
49	384377.0
50	396835.5
51	409294.0
52	413815.5
53	418337.0
54	418337.0
55	411846.5
56	405356.0
57	387990.0
58	370624.0
59	360021.0
60	349418.0
61	349418.0
62	327213.0
63	305008.0
64	269990.5
65	234973.0
66	207382.5
67	179792.0
68	179792.0
69	150328.0
70	120864.0
71	101952.0
72	83040.0
73	65610.5
74	48181.0
75	48181.0
76	37791.5
77	27402.0
78	21804.0
79	16206.0
80	12793.0
81	9380.0
82	9380.0
83	7311.5
84	5243.0
85	4040.5
86	2838.0
87	2145.0
88	1452.0
89	1452.0
90	1057.0
91	662.0
92	398.0
93	134.0
94	85.5
95	37.0
96	37.0
97	25.0
98	13.0
99	9.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5256571.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.67856370653471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.99793112947626	49.025585855751466
2	8.318485380820968	9.5959657795912
3	2.5376705435239533	4.391075763325287
4	1.1905595144953063	2.7467905121295426
5	0.6663329523754118	1.9216563821674275
6	0.40649348824662657	1.4067576334874745
7	0.28783722180577653	1.1621426274525515
8	0.21649704901810646	0.9989791067254099
9	0.15437774633829623	0.8013858009340208
>10	1.0165248843757488	11.271046479473743
>50	0.11749494234146286	4.769870574272215
>100	0.0845161993298015	9.279053624819648
>500	0.004415725118449903	1.6846286982097503
>1k	7.968209848828421E-4	0.7205873528836186
>5k	6.64017487397566E-5	0.224473808776755
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6250	0.11889880304099384	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5472	0.10409828003845092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.9023808486559012E-5	0.0
5	0.0	0.0	0.0	1.9023808486559012E-5	0.0
6	0.0	0.0	0.0	1.9023808486559012E-5	1.9023808486559012E-5
7	0.0	0.0	0.0	1.9023808486559012E-5	1.9023808486559012E-5
8	0.0	0.0	0.0	1.9023808486559012E-5	5.707142545967704E-5
9	0.0	0.0	0.0	5.707142545967704E-5	5.707142545967704E-5
10	0.0	1.9023808486559012E-5	0.0	5.707142545967704E-5	7.609523394623605E-5
11	0.0	1.9023808486559012E-5	0.0	7.609523394623605E-5	7.609523394623605E-5
12	0.0	1.9023808486559012E-5	0.0	1.1414285091935408E-4	9.511904243279507E-5
13	0.0	3.8047616973118025E-5	0.0	1.1414285091935408E-4	9.511904243279507E-5
14	0.0	3.8047616973118025E-5	0.0	1.1414285091935408E-4	9.511904243279507E-5
15	0.0	3.8047616973118025E-5	0.0	1.3316665940591308E-4	1.3316665940591308E-4
16	0.0	3.8047616973118025E-5	0.0	2.2828570183870816E-4	1.9023808486559013E-4
17	0.0	3.8047616973118025E-5	0.0	3.994999782177393E-4	1.9023808486559013E-4
18	0.0	3.8047616973118025E-5	0.0	4.375475951908573E-4	2.2828570183870816E-4
19	0.0	3.8047616973118025E-5	0.0	4.7559521216397534E-4	2.2828570183870816E-4
20	0.0	3.8047616973118025E-5	0.0	5.897380630833294E-4	2.6633331881182617E-4
21	0.0	3.8047616973118025E-5	0.0	7.799761479489195E-4	4.185237867042983E-4
22	0.0	3.8047616973118025E-5	0.0	0.0010272856582741866	4.185237867042983E-4
23	0.0	7.609523394623605E-5	0.0	0.0015409284874112802	4.185237867042983E-4
24	0.0	7.609523394623605E-5	0.0	0.002454071294766113	4.185237867042983E-4
25	0.0	7.609523394623605E-5	0.0	0.0030818569748225604	4.185237867042983E-4
26	0.0	7.609523394623605E-5	0.0	0.004698880696180076	4.565714036774163E-4
27	0.0	7.609523394623605E-5	0.0	0.016683880042712255	5.136428291370933E-4
28	0.0	7.609523394623605E-5	0.0	0.05731873497000231	5.136428291370933E-4
29	0.0	9.511904243279507E-5	0.0	0.1221138266752223	5.136428291370933E-4
30	0.0	1.1414285091935408E-4	0.0	0.21500708351508996	5.136428291370933E-4
31	0.0	1.1414285091935408E-4	0.0	0.5179612336635423	5.136428291370933E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2455	0.0	27.12831	1
CTTATAC	3415	0.0	18.906296	37
GTATCAA	3680	0.0	17.59511	2
TCTAGAC	1220	0.0	16.680326	3
TAGAACT	1235	0.0	15.7287445	4
GTCTAGA	1135	0.0	15.484581	1
TCTTATA	5740	0.0	15.277004	37
GTTCTAG	1260	0.0	15.123015	1
CTAGAAC	1325	0.0	14.381133	3
CTAGACA	1460	0.0	14.318493	4
ACTGATC	1230	0.0	14.138211	8
CAACGGA	1030	0.0	13.830097	24
GCAATTA	1085	0.0	13.81106	17
ACGGAGT	990	0.0	13.641415	26
CTCTAGT	1200	0.0	13.566666	27
TAGACAT	1420	0.0	13.549296	5
CTCTTAT	8975	0.0	13.295265	37
GCCGTAT	515	0.0	13.291263	8
AGCGTCA	590	0.0	13.169492	3
TTCTAGA	1690	0.0	13.136095	2
>>END_MODULE