FastQCFastQC Report
Fri 10 Feb 2017
ERR1632317.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632317.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252248
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA3550.14073451523897118No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC3520.13954520947638832No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG3180.12606641083378262No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT3180.12606641083378262TruSeq Adapter, Index 6 (95% over 21bp)
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG2860.11338048269956551No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG2680.10624464812406836No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT2570.10188386032793123No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG405.9220387E-527.7500021
ATATCAC451.3192152E-424.6666663
CTATATC502.6940063E-422.21
GGTATCA957.0776878E-921.4210531
TATATCA555.127809E-420.1818182
TTAGGAC1052.2386303E-819.380953
GCTTAGG902.138695E-618.51
TATTCAA609.2095917E-418.510
TCACTAT701.21441524E-418.56
GGATTAT500.007020036718.51
CTTATAC1900.017.52631637
TATCACT752.0592165E-417.2666664
TAGGAGT752.0592165E-417.2666664
GAATAGG650.00157531617.0769211
TAGGACA1005.8412807E-616.6500024
TCAACGC2700.016.4444453
TTCCTCG904.423859E-516.44444519
CTCTTAT5650.016.37168137
TATCAAC2600.016.3653831
CAACGCA2600.016.3653834
Produced by FastQC (version 0.11.5)