##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632317.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	252248
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.690439567409854	34.0	31.0	34.0	31.0	34.0
2	32.85836954108655	34.0	31.0	34.0	31.0	34.0
3	32.865568805302715	34.0	31.0	34.0	31.0	34.0
4	36.33746551013289	37.0	37.0	37.0	35.0	37.0
5	36.244925628746316	37.0	37.0	37.0	35.0	37.0
6	36.27918952776633	37.0	37.0	37.0	35.0	37.0
7	36.26787526561162	37.0	37.0	37.0	35.0	37.0
8	36.241873077289014	37.0	36.0	37.0	35.0	37.0
9	38.03203593289144	39.0	38.0	39.0	35.0	39.0
10	37.958663696045164	39.0	38.0	39.0	35.0	39.0
11	38.07945355364562	39.0	38.0	39.0	37.0	39.0
12	38.03696362310107	39.0	38.0	39.0	35.0	39.0
13	38.056674383939615	39.0	38.0	39.0	35.0	39.0
14	39.44953379214107	40.0	39.0	41.0	37.0	41.0
15	39.47351812501982	40.0	39.0	41.0	37.0	41.0
16	39.36759062509911	40.0	39.0	41.0	37.0	41.0
17	39.40069296882433	40.0	39.0	41.0	37.0	41.0
18	39.412153912023086	40.0	39.0	41.0	37.0	41.0
19	39.469629095176174	40.0	39.0	41.0	37.0	41.0
20	39.44207684500967	40.0	39.0	41.0	37.0	41.0
21	39.41762868288351	40.0	39.0	41.0	37.0	41.0
22	39.40202102692588	40.0	39.0	41.0	37.0	41.0
23	39.36453807364181	40.0	39.0	41.0	37.0	41.0
24	39.31807982620278	40.0	39.0	41.0	36.0	41.0
25	39.25483651010117	40.0	39.0	41.0	36.0	41.0
26	39.17200136373727	40.0	39.0	41.0	36.0	41.0
27	39.11035568171006	40.0	39.0	41.0	36.0	41.0
28	39.0524761345977	40.0	39.0	41.0	36.0	41.0
29	38.928487044495895	40.0	39.0	41.0	35.0	41.0
30	38.88802289810028	40.0	38.0	41.0	35.0	41.0
31	38.821913355110844	40.0	38.0	41.0	35.0	41.0
32	38.75409914052837	40.0	38.0	41.0	35.0	41.0
33	38.643275665218354	40.0	38.0	41.0	35.0	41.0
34	38.638197329612126	40.0	38.0	41.0	35.0	41.0
35	38.62934889473851	40.0	38.0	41.0	35.0	41.0
36	38.60257365767023	40.0	38.0	41.0	35.0	41.0
37	38.57904918968634	40.0	38.0	41.0	35.0	41.0
38	38.52031730677746	40.0	38.0	41.0	35.0	41.0
39	38.363075227553836	40.0	38.0	41.0	34.0	41.0
40	38.382381624433094	40.0	38.0	41.0	34.0	41.0
41	38.40789619739304	40.0	38.0	41.0	34.0	41.0
42	38.31218086962037	40.0	38.0	41.0	34.0	41.0
43	37.46229900732612	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	2.0
17	2.0
18	9.0
19	9.0
20	15.0
21	35.0
22	66.0
23	100.0
24	152.0
25	219.0
26	345.0
27	471.0
28	784.0
29	1020.0
30	1515.0
31	2089.0
32	3018.0
33	4067.0
34	6169.0
35	9358.0
36	15066.0
37	27173.0
38	65661.0
39	114902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04850781770321	17.15177127271574	12.48334971932384	27.316371190257204
2	19.746440011417334	20.014430243252672	33.19709175097523	27.04203799435476
3	20.843376359772922	20.645555167929974	29.396863404268814	29.114205068028287
4	16.177729853160383	14.96463797532587	31.78855729282294	37.06907487869081
5	16.754939583267262	34.54457517998161	32.750705654752466	15.94977958199867
6	37.37393358916622	32.17627097142495	14.791395769242968	15.658399670165867
7	31.463083949129427	28.457708287082557	19.57954076940154	20.499666994386477
8	28.469601344708384	31.908677174843803	19.3048111382449	20.316910342202913
9	27.55740382480733	13.74441026291586	17.907376867210047	40.79080904506676
10	18.01679299736767	25.934001458881735	31.63355110843297	24.415654435317624
11	38.01259078367321	19.934746757159623	19.843170213440743	22.209492245726427
12	23.491563857790744	23.45509181440487	27.123307221464593	25.930037106339793
13	32.58499571849926	17.9244235831404	22.682835939234405	26.80774475912594
14	24.72804541562272	18.976959183026228	22.53813707145349	33.75685832989756
15	27.578811328533824	25.671164885350922	20.316117471694522	26.43390631442073
16	27.57246836446672	23.97878278519552	21.532380831562588	26.916368018775174
17	26.06958231581618	24.631315213599315	22.89611810599093	26.402984364593575
18	25.585138435190764	23.19582315816181	24.317734927531635	26.90130347911579
19	27.206955060099585	23.31990739272462	23.968871903840665	25.504265643335128
20	27.428562367194193	22.95320478259491	23.8761060543592	25.7421267958517
21	27.138371761123974	23.50662839745013	22.58927721924455	26.765722622181343
22	27.455519964479404	22.977387333100758	22.764105166344233	26.802987536075605
23	26.72806127303289	23.508214138466908	22.694728996860235	27.068995591639972
24	27.743728394278644	23.075703276140942	22.868367638197327	26.31220069138308
25	27.432130284481936	22.585312866702612	23.145475880879136	26.837080967936316
26	27.118153563160064	22.78789128159589	23.140322222574607	26.953632932669436
27	26.73162919032064	22.844977958199866	23.115346801560367	27.308046049919128
28	26.37483746154578	23.097507215121627	23.53081094795598	26.99684437537661
29	26.269782119184292	23.565697250325076	23.38333703339571	26.78118359709492
30	26.27572864799721	24.184929117376548	23.06262091275253	26.476721321873715
31	26.715375344898675	23.371047540515683	23.32625035679173	26.587326757793917
32	25.70922266975358	23.36351527068599	23.893549205543753	27.033712854016684
33	25.939155117186264	22.959151311407823	23.965303986552914	27.136389584853006
34	26.2277599822397	22.856871015825693	23.93081411943801	26.984554882496592
35	26.076718150391677	22.5999809711078	24.391868320065967	26.931432558434555
36	25.677507849418035	22.93338301988519	24.605546922076687	26.783562208620086
37	26.345104817481207	21.551013288509722	24.86481557831975	27.23906631568932
38	24.86045479052361	22.1282230186166	26.272953601217846	26.738368589641944
39	24.968285179664456	21.363499413275825	26.91319653674162	26.755018870318096
40	23.98433287875424	21.041593986870062	28.205575465414988	26.768497668960705
41	23.57322952015477	21.136342012622496	28.44898671149028	26.841441755732454
42	23.660841711331706	20.4909454187942	28.43828295962703	27.40992991024706
43	22.284418508769146	20.548428530652373	29.504693793409658	27.66245916716882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	4.5
4	8.0
5	8.0
6	9.5
7	11.0
8	11.0
9	11.0
10	24.5
11	38.0
12	38.0
13	51.5
14	65.0
15	133.0
16	201.0
17	221.5
18	242.0
19	242.0
20	311.5
21	381.0
22	517.0
23	653.0
24	826.5
25	1000.0
26	1000.0
27	1277.0
28	1554.0
29	2232.5
30	2911.0
31	3445.0
32	3979.0
33	3979.0
34	4706.5
35	5434.0
36	6211.5
37	6989.0
38	8530.0
39	10071.0
40	10071.0
41	11478.5
42	12886.0
43	13087.0
44	13288.0
45	14752.5
46	16217.0
47	16217.0
48	17487.0
49	18757.0
50	20422.5
51	22088.0
52	23611.0
53	25134.0
54	25134.0
55	23116.0
56	21098.0
57	19986.5
58	18875.0
59	17509.5
60	16144.0
61	16144.0
62	15209.5
63	14275.0
64	12992.5
65	11710.0
66	10267.5
67	8825.0
68	8825.0
69	7662.5
70	6500.0
71	5711.0
72	4922.0
73	4087.5
74	3253.0
75	3253.0
76	2610.0
77	1967.0
78	1657.5
79	1348.0
80	1031.0
81	714.0
82	714.0
83	550.0
84	386.0
85	284.5
86	183.0
87	124.5
88	66.0
89	66.0
90	55.5
91	45.0
92	28.5
93	12.0
94	8.5
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	252248.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.49434879463281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.74194897895896	67.42514797359017
2	4.946226140443981	7.270393383658409
3	1.1860943797779484	2.6151370215226266
4	0.5693561650954635	1.6737784234360218
5	0.32170592500541795	1.1821783730824087
6	0.19811442944552876	0.8736174587351618
7	0.14772021047836661	0.7599620471039555
8	0.1043167488393816	0.6133353219458896
9	0.08245376490525687	0.5453897181639842
>10	0.5877742346122221	8.715506843650209
>50	0.0758315788684774	3.8232100598072036
>100	0.03845744356901354	4.502343375303985
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	355	0.14073451523897118	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	352	0.13954520947638832	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	318	0.12606641083378262	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	318	0.12606641083378262	TruSeq Adapter, Index 6 (95% over 21bp)
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	286	0.11338048269956551	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	268	0.10624464812406836	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	257	0.10188386032793123	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	3.96435254194285E-4	0.0
21	0.0	0.0	0.0	7.9287050838857E-4	0.0
22	0.0	0.0	0.0	0.001189305762582855	0.0
23	0.0	0.0	0.0	0.00158574101677714	0.0
24	0.0	0.0	0.0	0.002775046779359995	0.0
25	0.0	0.0	0.0	0.003567917287748565	0.0
26	0.0	0.0	0.0	0.0059465288129142745	0.0
27	0.0	0.0	0.0	0.020218197963908533	0.0
28	0.0	0.0	0.0	0.09316228473565698	0.0
29	0.0	0.0	0.0	0.1875138752338968	0.0
30	0.0	0.0	0.0	0.30010148742507375	0.0
31	0.0	0.0	0.0	0.6438108528115188	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCTAG	40	5.9220387E-5	27.750002	1
ATATCAC	45	1.3192152E-4	24.666666	3
CTATATC	50	2.6940063E-4	22.2	1
GGTATCA	95	7.0776878E-9	21.421053	1
TATATCA	55	5.127809E-4	20.181818	2
TTAGGAC	105	2.2386303E-8	19.38095	3
GCTTAGG	90	2.138695E-6	18.5	1
TATTCAA	60	9.2095917E-4	18.5	10
TCACTAT	70	1.21441524E-4	18.5	6
GGATTAT	50	0.0070200367	18.5	1
CTTATAC	190	0.0	17.526316	37
TATCACT	75	2.0592165E-4	17.266666	4
TAGGAGT	75	2.0592165E-4	17.266666	4
GAATAGG	65	0.001575316	17.076921	1
TAGGACA	100	5.8412807E-6	16.650002	4
TCAACGC	270	0.0	16.444445	3
TTCCTCG	90	4.423859E-5	16.444445	19
CTCTTAT	565	0.0	16.371681	37
TATCAAC	260	0.0	16.365383	1
CAACGCA	260	0.0	16.365383	4
>>END_MODULE