FastQCFastQC Report
Fri 10 Feb 2017
ERR1632316.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632316.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714894
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.2868981989557372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44290.25826669170222766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34850.20321955759364718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12050.025.0248971
GTATCAA18850.015.7029182
TCTTATA14000.014.53571537
TCAATAC1405.9993E-714.5357153
GTATTAT2059.276846E-1114.4390251
TTGTACT2900.013.3965514
GTACTAT1805.1626557E-813.3611111
CTCTTAT21800.013.06880937
CGTGTAA850.00940719713.0588241
GGACTAG1857.388371E-813.01
ATACGCT1000.001833119212.9500014
CATCGTA1153.5787662E-412.8695659
TGTACCG1153.5787662E-412.8695655
TAATACT2501.6007107E-1012.584
TAACACA2501.6007107E-1012.584
CCAGTAC3250.012.5230773
CCGATCG1205.123729E-412.33333418
CGATCGC1205.123729E-412.33333419
TACCGTC1050.002644483712.3333337
GTTATAT2002.0411062E-712.0250011
Produced by FastQC (version 0.11.5)