##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632316.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1714894
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219445050248005	31.0	31.0	34.0	30.0	34.0
2	31.384568958781127	31.0	31.0	34.0	30.0	34.0
3	31.428965288816684	31.0	31.0	34.0	30.0	34.0
4	35.29188509610506	37.0	35.0	37.0	33.0	37.0
5	34.31302634448543	35.0	35.0	37.0	30.0	37.0
6	34.251910030590814	35.0	35.0	37.0	30.0	37.0
7	34.26215906056001	35.0	35.0	37.0	30.0	37.0
8	34.19214890249776	35.0	35.0	37.0	30.0	37.0
9	35.72778025930466	37.0	35.0	39.0	30.0	39.0
10	35.36098616007753	37.0	34.0	39.0	30.0	39.0
11	35.600617297628894	37.0	35.0	39.0	30.0	39.0
12	35.49626099339085	37.0	35.0	39.0	30.0	39.0
13	35.6391899441015	37.0	35.0	39.0	30.0	39.0
14	36.55952904377763	38.0	36.0	40.0	31.0	41.0
15	36.51070036981878	38.0	36.0	40.0	31.0	41.0
16	36.39978564272777	38.0	35.0	40.0	30.0	41.0
17	36.39583321184866	38.0	35.0	40.0	30.0	41.0
18	36.41387106141838	38.0	35.0	40.0	30.0	41.0
19	36.490403488495495	38.0	35.0	40.0	30.0	41.0
20	36.44450619105321	38.0	35.0	40.0	30.0	41.0
21	36.3970321197695	38.0	35.0	40.0	30.0	41.0
22	36.36545757347101	38.0	35.0	40.0	30.0	41.0
23	36.29315514544923	38.0	35.0	40.0	30.0	41.0
24	36.188362079522115	38.0	34.0	40.0	30.0	41.0
25	36.090364185774746	38.0	34.0	40.0	29.0	41.0
26	35.91873608514579	38.0	34.0	40.0	29.0	41.0
27	35.79528530626383	38.0	34.0	40.0	29.0	41.0
28	35.67260017237217	38.0	34.0	40.0	28.0	41.0
29	35.483685872129705	38.0	34.0	40.0	27.0	41.0
30	35.372476083069856	38.0	34.0	40.0	27.0	41.0
31	35.228414117723894	38.0	34.0	40.0	27.0	41.0
32	35.105342371015354	38.0	33.0	40.0	27.0	41.0
33	34.91393054031328	37.0	33.0	40.0	26.0	41.0
34	34.873136765304444	37.0	33.0	40.0	26.0	41.0
35	34.82025303021644	37.0	33.0	40.0	26.0	40.0
36	34.77426359879969	37.0	33.0	40.0	26.0	40.0
37	34.726034961927674	37.0	33.0	39.0	26.0	40.0
38	34.61761251715849	37.0	33.0	39.0	25.0	40.0
39	34.401947292369094	37.0	33.0	39.0	25.0	40.0
40	34.363803826942075	37.0	33.0	39.0	25.0	40.0
41	34.3188704374731	37.0	33.0	39.0	24.0	40.0
42	34.16387835049863	37.0	32.0	39.0	24.0	40.0
43	33.23984631120057	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	5.0
13	9.0
14	14.0
15	40.0
16	83.0
17	184.0
18	384.0
19	659.0
20	1217.0
21	2047.0
22	3257.0
23	5297.0
24	8001.0
25	11957.0
26	17179.0
27	24079.0
28	33015.0
29	44858.0
30	59063.0
31	76881.0
32	99960.0
33	127665.0
34	161994.0
35	201620.0
36	241853.0
37	263497.0
38	232631.0
39	97439.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.90398940109418	19.585525402736263	12.631567898657291	25.878917297512267
2	19.48936785597244	21.090633007054663	33.761212063252884	25.658787073720006
3	20.81259832969268	22.821527161445548	29.07427514470282	27.29159936415895
4	15.796544859332414	16.190388443833847	33.754855985267895	34.258210711565845
5	15.913053518176634	35.1007700767511	33.123271759070825	15.862904646001445
6	34.72068827577681	34.56161138822574	15.53203871492932	15.185661621068125
7	29.69116458509972	29.680376746317847	21.18428311020973	19.444175558372702
8	27.508347454711483	33.19254717784306	19.584767338389426	19.714338029056023
9	26.825331478213815	14.313246183146013	19.691712723935122	39.16970961470505
10	17.426616455594342	26.625610679144017	32.67688848406957	23.270884381192072
11	36.61748189684027	21.376598203737373	21.210407173854477	20.795512725567878
12	22.04999259429446	24.958568867813405	29.03024909994437	23.96118943794777
13	31.02693227686376	19.518757427572783	24.023117463819922	25.431192831743537
14	23.01016855852315	20.13308111171886	25.07851797253941	31.778232357218577
15	25.670041413638394	27.07543440002706	21.890041017112427	25.36448316922212
16	25.32547201168119	25.648232485506394	23.883342060792096	25.14295344202032
17	24.053498350335357	26.029772102532284	25.40285288770035	24.513876659432015
18	24.409846905989525	24.942066390109243	26.108144293466534	24.539942410434698
19	24.96422519409363	25.009709054903684	26.133685230690645	23.89238052031204
20	25.03641624496908	24.871449780569527	25.86451407492241	24.22761989953898
21	25.28552785186723	24.84241008482157	25.500060062021323	24.372002001289875
22	25.405127080740854	24.76742002712704	25.430784643249087	24.39666824888302
23	25.05898323744791	24.7777413647724	25.546360299820282	24.616915097959406
24	24.784797194462165	25.018397638571248	25.30646209036827	24.89034307659832
25	25.05455147665103	24.548747619386386	25.56641984869036	24.83028105527222
26	24.832496935670658	24.965566384861106	25.536388838027307	24.66554784144093
27	24.765320772012732	24.82958130356745	25.494870236877613	24.910227687542204
28	24.640590030637462	24.913318257571603	25.8078924994781	24.63819921231283
29	24.659716577234512	24.94387408201323	25.811216320075758	24.585193020676495
30	24.48104664194988	25.14470282128225	25.614586091035363	24.759664445732508
31	24.835937381552444	24.91069418867872	25.553241191583854	24.700127238184987
32	24.27496976489509	25.03058498076266	25.706603440212632	24.98784181412962
33	24.28004296475467	24.890401389240385	25.97268402595146	24.856871620053482
34	24.947314527895017	24.37754170228597	25.732494253289122	24.942649516529887
35	24.437662036254135	24.71808753194075	26.17164676067442	24.672603671130695
36	24.498248871358815	24.622396486313438	25.81039994308686	25.068954699240887
37	24.397951127008433	23.97028621010978	26.394750929211952	25.237011733669835
38	23.94976016010319	24.213858116011835	26.841717330633845	24.994664393251128
39	23.851211795014734	23.966437575733543	26.986332682953	25.19601794629872
40	23.883225435507967	23.752663429926283	27.56514396808199	24.79896716648376
41	23.349023321558068	23.63125650914867	27.895718335943798	25.124001833349467
42	23.24371068999017	23.81062619613807	27.848718346440073	25.09694476743169
43	23.064282690358702	23.303014646969434	28.171420507623214	25.461282155048647
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	148.5
2	190.0
3	447.5
4	705.0
5	705.0
6	941.5
7	1178.0
8	1165.0
9	1152.0
10	1614.0
11	2076.0
12	2076.0
13	3655.0
14	5234.0
15	7893.5
16	10553.0
17	10332.5
18	10112.0
19	10112.0
20	12125.0
21	14138.0
22	13617.5
23	13097.0
24	15560.0
25	18023.0
26	18023.0
27	21677.5
28	25332.0
29	30004.5
30	34677.0
31	40026.0
32	45375.0
33	45375.0
34	52348.0
35	59321.0
36	65981.0
37	72641.0
38	78874.0
39	85107.0
40	85107.0
41	91141.5
42	97176.0
43	101772.0
44	106368.0
45	110528.5
46	114689.0
47	114689.0
48	117158.5
49	119628.0
50	120553.0
51	121478.0
52	121876.0
53	122274.0
54	122274.0
55	119888.5
56	117503.0
57	113061.0
58	108619.0
59	104116.5
60	99614.0
61	99614.0
62	92162.5
63	84711.0
64	76057.0
65	67403.0
66	59144.5
67	50886.0
68	50886.0
69	44026.5
70	37167.0
71	31586.0
72	26005.0
73	21329.0
74	16653.0
75	16653.0
76	13570.0
77	10487.0
78	8512.0
79	6537.0
80	5303.0
81	4069.0
82	4069.0
83	3168.5
84	2268.0
85	1756.0
86	1244.0
87	957.5
88	671.0
89	671.0
90	493.0
91	315.0
92	195.5
93	76.0
94	51.0
95	26.0
96	26.0
97	17.5
98	9.0
99	4.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1714894.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.50797912972399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.41534620322143	62.508947519175564
2	7.650692492973915	10.941711182310296
3	2.052225368932944	4.402514665534413
4	0.8745524169967563	2.5014990392981495
5	0.4998692524580033	1.7872320036178817
6	0.3134372068996423	1.344795674967516
7	0.20683697201806026	1.0353345714825932
8	0.14420368720203974	0.8249371403898165
9	0.10909556858349112	0.702108327727229
>10	0.6640329529492758	8.852887292093326
>50	0.054102666779320616	2.6257646183657677
>100	0.01511513469205115	1.563801200962731
>500	1.6335876435543824E-4	0.06261259940131791
>1k	3.267175287108764E-4	0.845854164673401
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4920	0.2868981989557372	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4429	0.25826669170222766	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3485	0.20321955759364718	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	5.831264206417423E-5	0.0	0.0	0.0
4	0.0	5.831264206417423E-5	0.0	0.0	0.0
5	0.0	5.831264206417423E-5	0.0	5.831264206417423E-5	0.0
6	0.0	5.831264206417423E-5	0.0	5.831264206417423E-5	0.0
7	0.0	5.831264206417423E-5	0.0	1.1662528412834846E-4	0.0
8	0.0	5.831264206417423E-5	0.0	1.1662528412834846E-4	0.0
9	0.0	5.831264206417423E-5	0.0	2.332505682566969E-4	0.0
10	0.0	5.831264206417423E-5	0.0	2.915632103208711E-4	0.0
11	0.0	5.831264206417423E-5	0.0	3.498758523850454E-4	0.0
12	0.0	5.831264206417423E-5	0.0	4.081884944492196E-4	0.0
13	0.0	5.831264206417423E-5	0.0	4.081884944492196E-4	0.0
14	0.0	5.831264206417423E-5	0.0	4.665011365133938E-4	0.0
15	0.0	5.831264206417423E-5	0.0	6.997517047700908E-4	0.0
16	0.0	5.831264206417423E-5	0.0	8.163769888984392E-4	0.0
17	0.0	5.831264206417423E-5	0.0	0.0011079401992193103	0.0
18	0.0	5.831264206417423E-5	0.0	0.0011079401992193103	0.0
19	0.0	5.831264206417423E-5	0.0	0.0011662528412834845	0.0
20	0.0	5.831264206417423E-5	0.0	0.0013411907674760074	0.0
21	0.0	5.831264206417423E-5	0.0	0.0018660045460535753	0.0
22	0.0	5.831264206417423E-5	0.0	0.002565756250823666	0.0
23	0.0	1.1662528412834846E-4	0.0	0.0031488826714654084	0.0
24	0.0	1.1662528412834846E-4	0.0	0.004373448154813067	0.0
25	0.0	1.1662528412834846E-4	0.0	0.005073199859583158	0.0
26	0.0	1.1662528412834846E-4	0.0	0.007114142331829256	0.0
27	0.0	1.1662528412834846E-4	0.0	0.019243171881177495	0.0
28	0.0	1.749379261925227E-4	0.0	0.05854589263243093	0.0
29	0.0	1.749379261925227E-4	0.0	0.11306821296243383	0.0
30	0.0	1.749379261925227E-4	0.0	0.18485107534343231	0.0
31	0.0	1.749379261925227E-4	0.0	0.3819478055203412	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1205	0.0	25.024897	1
GTATCAA	1885	0.0	15.702918	2
TCTTATA	1400	0.0	14.535715	37
TCAATAC	140	5.9993E-7	14.535715	3
GTATTAT	205	9.276846E-11	14.439025	1
TTGTACT	290	0.0	13.396551	4
GTACTAT	180	5.1626557E-8	13.361111	1
CTCTTAT	2180	0.0	13.068809	37
CGTGTAA	85	0.009407197	13.058824	1
GGACTAG	185	7.388371E-8	13.0	1
ATACGCT	100	0.0018331192	12.950001	4
CATCGTA	115	3.5787662E-4	12.869565	9
TGTACCG	115	3.5787662E-4	12.869565	5
TAATACT	250	1.6007107E-10	12.58	4
TAACACA	250	1.6007107E-10	12.58	4
CCAGTAC	325	0.0	12.523077	3
CCGATCG	120	5.123729E-4	12.333334	18
CGATCGC	120	5.123729E-4	12.333334	19
TACCGTC	105	0.0026444837	12.333333	7
GTTATAT	200	2.0411062E-7	12.025001	1
>>END_MODULE