FastQCFastQC Report
Fri 10 Feb 2017
ERR1632315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54055
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT1590.2941448524650819TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT690.12764776616409212No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC650.12024789566182591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGA250.00545069829.5999981
GTCCAGT250.00545069829.5999981
GCCCACG450.00377953620.5555558
TGTCTCT2700.016.44444537
CTCTTAT700.002549894215.85714337
ACCTGTC750.00403751714.836
GTCTCTT1552.6091198E-511.93548437
CTGTCTC3950.011.24050636
TCTGTCT1450.002427346510.20689736
GCTGTCT1650.0070563068.96969737
CCTGTCT2301.4150243E-48.84782537
Produced by FastQC (version 0.11.5)