##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632315.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	54055
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.771732494681345	34.0	31.0	34.0	31.0	34.0
2	32.92783276292665	34.0	31.0	34.0	31.0	34.0
3	32.93216168717048	34.0	33.0	34.0	31.0	34.0
4	36.37548792896124	37.0	37.0	37.0	35.0	37.0
5	36.28900194246601	37.0	37.0	37.0	35.0	37.0
6	36.31997040051799	37.0	37.0	37.0	35.0	37.0
7	36.31188604199426	37.0	37.0	37.0	35.0	37.0
8	36.29342336509111	37.0	37.0	37.0	35.0	37.0
9	38.09177689390436	39.0	39.0	39.0	37.0	39.0
10	38.0060493941356	39.0	38.0	39.0	35.0	39.0
11	38.13129220238646	39.0	38.0	39.0	37.0	39.0
12	38.09138840070299	39.0	38.0	39.0	37.0	39.0
13	38.123374340949034	39.0	38.0	39.0	37.0	39.0
14	39.54331699195264	40.0	39.0	41.0	37.0	41.0
15	39.54135602626954	41.0	39.0	41.0	37.0	41.0
16	39.42565905096661	40.0	39.0	41.0	37.0	41.0
17	39.4744426972528	40.0	39.0	41.0	37.0	41.0
18	39.497641291277404	41.0	39.0	41.0	37.0	41.0
19	39.536416612709274	41.0	39.0	41.0	37.0	41.0
20	39.51911941541023	41.0	39.0	41.0	37.0	41.0
21	39.49038941818518	41.0	39.0	41.0	37.0	41.0
22	39.47568217556193	41.0	39.0	41.0	37.0	41.0
23	39.44599019517158	40.0	39.0	41.0	37.0	41.0
24	39.40373693460364	41.0	39.0	41.0	37.0	41.0
25	39.33680510591065	40.0	39.0	41.0	36.0	41.0
26	39.25418555175285	40.0	39.0	41.0	36.0	41.0
27	39.18880769586532	40.0	39.0	41.0	36.0	41.0
28	39.13889556932754	40.0	39.0	41.0	36.0	41.0
29	38.99722504856165	40.0	39.0	41.0	35.0	41.0
30	38.96831005457405	40.0	39.0	41.0	35.0	41.0
31	38.90687262972898	40.0	38.0	41.0	35.0	41.0
32	38.83733234668393	40.0	38.0	41.0	35.0	41.0
33	38.71630746461937	40.0	38.0	41.0	35.0	41.0
34	38.71963740634539	40.0	38.0	41.0	35.0	41.0
35	38.70729812228286	40.0	38.0	41.0	35.0	41.0
36	38.680399593007124	40.0	38.0	41.0	35.0	41.0
37	38.65542503006198	40.0	38.0	41.0	35.0	41.0
38	38.593802608454354	40.0	38.0	41.0	35.0	41.0
39	38.42134862639904	40.0	38.0	41.0	34.0	41.0
40	38.43729534733142	40.0	38.0	41.0	34.0	41.0
41	38.44819165664601	40.0	38.0	41.0	34.0	41.0
42	38.332124687817966	40.0	38.0	41.0	34.0	41.0
43	37.49172139487559	39.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	2.0
21	4.0
22	8.0
23	21.0
24	24.0
25	42.0
26	77.0
27	101.0
28	134.0
29	225.0
30	284.0
31	430.0
32	593.0
33	853.0
34	1290.0
35	1920.0
36	3124.0
37	5537.0
38	13619.0
39	25766.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.3521413375266	17.64499121265378	11.960040699287761	27.042826750531866
2	20.34224401072981	19.583757284247525	32.4058828970493	27.668115807973358
3	21.200628988992694	20.884284525020814	28.524650818610674	29.39043566737582
4	15.739524558320229	15.042086763481638	32.59087965960596	36.62750901859218
5	17.078901119230412	33.70456017019702	32.47433169919526	16.7422070113773
6	37.064101378225885	32.206086393488114	14.66654333549163	16.063268892794376
7	30.992507631116457	28.1028581999815	20.196096568310054	20.70853760059199
8	28.409952825825545	30.874109703080194	19.535658125982795	21.18027934511146
9	27.020627139025066	13.497363796133568	18.668023309592083	40.813985755249284
10	18.268430302469707	25.14106003144945	31.941541023032098	24.648968643048747
11	37.64869114790491	20.344093978355378	19.81685320506891	22.1903616686708
12	23.36879104615669	24.136527610766812	27.218573674960687	25.27610766811581
13	32.55758024234576	18.375728424752566	22.06086393488114	27.005827398020532
14	24.804365923596336	19.507908611599298	22.708352603829432	32.979372860974934
15	27.129775228933497	25.734899639256316	20.42364258625474	26.711682545555455
16	27.39987050226621	24.145777448894645	21.944315974470445	26.5100360743687
17	25.146609934326147	24.423272592729628	24.058828970493014	26.371288502451208
18	25.86624734067154	23.544537970585512	23.90713162519656	26.682083063546386
19	26.64878364628619	23.805383405790398	24.11617796688558	25.42965498103783
20	26.336139117565445	23.20969383035797	24.14207751364351	26.312089538433074
21	27.386920728887244	23.572287484969014	23.18934418647674	25.851447599667004
22	27.608916843955235	23.48348903894182	23.115345481454074	25.792248635648875
23	26.807880862084914	23.47238923318842	23.163444639718804	26.556285265007862
24	26.794931088705948	23.489038941818517	23.35399130515216	26.36203866432337
25	26.63583387290722	23.31144204976413	23.59078716122468	26.461936916103966
26	26.60438442327259	23.679585607251873	23.522338359078717	26.19369161039682
27	26.251040606789385	23.67033576912404	23.605586902229213	26.47303672185737
28	25.992045139210063	23.196744056979	23.770234020904635	27.040976782906302
29	26.256590509666083	23.122745351956343	23.931181204328926	26.68948293404865
30	26.012394783091295	23.8109333086671	24.00887984460272	26.167792063638885
31	26.593284617519192	23.83128295254833	23.455739524558318	26.119692905374155
32	26.130792711127555	23.464989362686154	23.77393395615577	26.630283970030526
33	25.36675608176857	23.276292664878365	24.602719452409584	26.754231800943483
34	26.17519193414115	22.971048006659885	24.238275830172974	26.61548422902599
35	25.94949588382203	22.911849042641755	24.858014984737768	26.280640088798446
36	25.87549717879937	23.126445287207474	24.151327351771343	26.846730182221812
37	25.474054204051427	22.144112478031637	25.52030339469059	26.861529923226346
38	25.42965498103783	22.13671260752937	25.679400610489317	26.754231800943483
39	25.26500786236241	21.940616039219314	26.147442419757656	26.646933678660623
40	24.55092035889372	21.615021737119598	27.188974192951626	26.645083711035056
41	23.94968088058459	21.49662380908334	27.29072241235778	27.262972897974286
42	23.958930718712423	21.20802885949496	27.749514383498287	27.08352603829433
43	23.33179169364536	21.14327999260013	28.426602534455647	27.09832577929886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	2.0
6	5.0
7	8.0
8	6.5
9	5.0
10	8.5
11	12.0
12	12.0
13	22.0
14	32.0
15	52.5
16	73.0
17	78.0
18	83.0
19	83.0
20	105.5
21	128.0
22	150.0
23	172.0
24	223.0
25	274.0
26	274.0
27	361.5
28	449.0
29	543.5
30	638.0
31	791.0
32	944.0
33	944.0
34	1098.0
35	1252.0
36	1488.5
37	1725.0
38	1917.0
39	2109.0
40	2109.0
41	2398.0
42	2687.0
43	2955.5
44	3224.0
45	3370.5
46	3517.0
47	3517.0
48	3703.5
49	3890.0
50	4020.5
51	4151.0
52	4223.5
53	4296.0
54	4296.0
55	4403.0
56	4510.0
57	4330.0
58	4150.0
59	3961.0
60	3772.0
61	3772.0
62	3510.0
63	3248.0
64	2981.0
65	2714.0
66	2351.0
67	1988.0
68	1988.0
69	1703.5
70	1419.0
71	1212.0
72	1005.0
73	802.5
74	600.0
75	600.0
76	485.0
77	370.0
78	308.5
79	247.0
80	214.5
81	182.0
82	182.0
83	138.0
84	94.0
85	66.0
86	38.0
87	33.5
88	29.0
89	29.0
90	21.0
91	13.0
92	8.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	54055.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.01470724262325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.934753445262	86.4045879197114
2	3.3659904874181876	6.127092775876422
3	0.8597910484166024	2.347608916843955
4	0.3496077076303915	1.2727777263897881
5	0.19919508923126958	0.9064841365276107
6	0.1117931623236717	0.6104893164369624
7	0.05691288263750559	0.3625936546110443
8	0.03048904427009228	0.2219961150679863
9	0.01829342656205537	0.14984737767089076
>10	0.06707589739420301	1.054481546572935
>50	0.00406520590267897	0.24789566182591805
>100	0.002032602951339485	0.2941448524650819
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	159	0.2941448524650819	TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	69	0.12764776616409212	No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	65	0.12024789566182591	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0018499676255665527	0.0
17	0.0	0.0	0.0	0.0018499676255665527	0.0
18	0.0	0.0	0.0	0.0018499676255665527	0.0
19	0.0	0.0	0.0	0.0018499676255665527	0.0
20	0.0	0.0	0.0	0.0018499676255665527	0.0
21	0.0	0.0	0.0	0.0018499676255665527	0.0
22	0.0	0.0	0.0	0.0018499676255665527	0.0
23	0.0	0.0	0.0	0.0036999352511331053	0.0
24	0.0	0.0	0.0	0.0036999352511331053	0.0
25	0.0	0.0	0.0	0.0036999352511331053	0.0
26	0.0	0.0	0.0	0.005549902876699658	0.0
27	0.0	0.0	0.0	0.024049579132365183	0.0
28	0.0	0.0	0.0	0.05734899639256313	0.0
29	0.0	0.0	0.0	0.12024789566182592	0.0
30	0.0	0.0	0.0	0.1905466654333549	0.0
31	0.0	0.0	0.0	0.4143927481269078	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTGA	25	0.005450698	29.599998	1
GTCCAGT	25	0.005450698	29.599998	1
GCCCACG	45	0.003779536	20.555555	8
TGTCTCT	270	0.0	16.444445	37
CTCTTAT	70	0.0025498942	15.857143	37
ACCTGTC	75	0.004037517	14.8	36
GTCTCTT	155	2.6091198E-5	11.935484	37
CTGTCTC	395	0.0	11.240506	36
TCTGTCT	145	0.0024273465	10.206897	36
GCTGTCT	165	0.007056306	8.969697	37
CCTGTCT	230	1.4150243E-4	8.847825	37
>>END_MODULE