FastQCFastQC Report
Fri 10 Feb 2017
ERR1632314.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632314.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783278
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.2910843914931863No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16050.20490809138007196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15690.20031202204070586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.022.751
ACGTTAA450.003824735320.55555720
CGCCTAA450.003824735320.55555713
TAAGACT1101.7516868E-920.1818184
CTTATAC9500.018.69473637
CGTTATT1156.397204E-817.6956522
GCGTTAT953.6034216E-617.5263161
CTAGAAC1201.03991624E-716.9583323
TGACCCG904.443046E-516.44444513
TAGAACT1606.2755134E-1016.18754
CCAATAC700.002591504515.8571433
GTAAGAC1001.0926573E-414.83
GTACGGG750.004102853614.837
GTATCAA11400.014.7675442
GATTAAT1904.5474735E-1014.6052641
CGGACCA1304.439322E-614.2307699
TCTTATA15550.014.03858637
TCACGAG800.006296259413.87525
TTGGCGT800.006296259413.87523
ATAGCCG800.006296259413.8755
Produced by FastQC (version 0.11.5)