##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632314.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	783278
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64373313178718	34.0	31.0	34.0	31.0	34.0
2	32.81151902645038	34.0	31.0	34.0	31.0	34.0
3	32.820471148174725	34.0	31.0	34.0	31.0	34.0
4	36.306404622624406	37.0	37.0	37.0	35.0	37.0
5	36.21377467514726	37.0	35.0	37.0	35.0	37.0
6	36.23828449158536	37.0	36.0	37.0	35.0	37.0
7	36.22598362267292	37.0	36.0	37.0	35.0	37.0
8	36.213246127173235	37.0	36.0	37.0	35.0	37.0
9	37.979957307622584	39.0	38.0	39.0	35.0	39.0
10	37.90412727026675	39.0	38.0	39.0	35.0	39.0
11	38.01383289202556	39.0	38.0	39.0	35.0	39.0
12	37.97536123828321	39.0	38.0	39.0	35.0	39.0
13	38.00832526893389	39.0	38.0	39.0	35.0	39.0
14	39.37025423923562	40.0	39.0	41.0	37.0	41.0
15	39.3893789944311	40.0	39.0	41.0	37.0	41.0
16	39.27965805244115	40.0	39.0	41.0	36.0	41.0
17	39.32817467106187	40.0	39.0	41.0	36.0	41.0
18	39.34770158232454	40.0	39.0	41.0	36.0	41.0
19	39.40432260321367	40.0	39.0	41.0	37.0	41.0
20	39.381612913933495	40.0	39.0	41.0	37.0	41.0
21	39.357700586509516	40.0	39.0	41.0	37.0	41.0
22	39.3283700040088	40.0	39.0	41.0	36.0	41.0
23	39.282916154928394	40.0	39.0	41.0	36.0	41.0
24	39.22660153866188	40.0	39.0	41.0	36.0	41.0
25	39.164943991788356	40.0	39.0	41.0	36.0	41.0
26	39.072674069742796	40.0	39.0	41.0	36.0	41.0
27	39.0019890766752	40.0	39.0	41.0	35.0	41.0
28	38.93183007820978	40.0	38.0	41.0	35.0	41.0
29	38.7784898848174	40.0	38.0	41.0	35.0	41.0
30	38.73026435058817	40.0	38.0	41.0	35.0	41.0
31	38.64467787937361	40.0	38.0	41.0	35.0	41.0
32	38.568496753387684	40.0	38.0	41.0	35.0	41.0
33	38.427735746439964	40.0	38.0	41.0	34.0	41.0
34	38.417134146497155	40.0	38.0	41.0	34.0	41.0
35	38.386890733558204	40.0	38.0	41.0	34.0	41.0
36	38.36336013522657	40.0	38.0	41.0	34.0	41.0
37	38.33766683093359	40.0	38.0	41.0	34.0	41.0
38	38.27040846289567	40.0	38.0	41.0	34.0	41.0
39	38.08359994791122	40.0	38.0	41.0	34.0	41.0
40	38.0774565352276	40.0	37.0	41.0	34.0	41.0
41	38.07466952984764	40.0	37.0	41.0	34.0	41.0
42	37.93482390671001	40.0	37.0	41.0	33.0	41.0
43	37.085089840388726	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	1.0
17	4.0
18	16.0
19	20.0
20	45.0
21	108.0
22	168.0
23	321.0
24	523.0
25	893.0
26	1310.0
27	1922.0
28	2856.0
29	3988.0
30	5421.0
31	7767.0
32	10307.0
33	14300.0
34	21286.0
35	31606.0
36	50576.0
37	91510.0
38	202789.0
39	335539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.951927668082085	17.724358401487084	12.332147717668567	26.991566212762265
2	19.15973638988967	20.21542798342351	33.26315816351283	27.361677463173994
3	20.044990412088683	21.290908208835177	29.72469544657197	28.93940593250417
4	15.18222138244659	15.293931401111738	33.765534076024096	35.75831314041758
5	16.303151626880876	34.44971006462584	33.016119436521905	16.23101887197138
6	35.40747984751263	33.911842283327246	15.352658953781418	15.328018915378703
7	30.342611435531193	29.558087933019948	20.309009061916715	19.790291569532144
8	28.788501655861648	31.603849463408906	19.53686941290321	20.070779467826238
9	27.510794379517872	13.610876342754425	18.77519858849604	40.10313068923166
10	17.793427110170335	25.808333695061016	31.977407765825163	24.42083142894349
11	37.45017733167534	20.554898771572798	20.609285592088632	21.385638304663225
12	23.106483266477547	24.440492392228556	27.880394955558565	24.572629385735333
13	32.094607533978994	18.478879784699682	23.237854248427762	26.188658432893558
14	23.812490584441285	19.518357466952985	24.150429349477452	32.51872259912828
15	26.442463595300776	26.10695053352705	21.28490778497545	26.165678086196724
16	25.904723482594942	24.930356782649326	23.119122457160802	26.04579727759493
17	24.803581870038478	25.130413467504514	24.612844992454786	25.453159670002222
18	25.0919852210837	23.93645678801141	25.356386876690014	25.615171114214874
19	25.623214235558766	24.20877389636885	25.41383774343209	24.754174124640294
20	26.174870224875455	23.864068695916394	24.81124198560409	25.14981909360406
21	26.56591912449986	23.957522105816835	24.189878944640345	25.286679825042963
22	26.788573150273592	24.116597172396006	24.0518691958666	25.0429604814638
23	26.299602440002147	23.89394314662227	24.071402490558906	25.735051922816677
24	25.63904514106103	23.97986410954987	24.515816861957056	25.86527388743205
25	25.726881132880024	23.5088946708576	24.5333074591652	26.230916737097175
26	25.189396357359712	24.10970306838696	24.558713509124473	26.14218706512886
27	25.275827994658346	24.258181641767035	24.514923181807735	25.951067181766884
28	25.388814699251093	24.162685534382426	24.745620328925362	25.702879437441112
29	25.070664566092756	24.515433856178777	25.026874238776013	25.387027338952457
30	25.196928804332565	24.6086319288937	24.573906071662936	25.620533195110806
31	25.276338669029386	24.470877517305478	24.393382681500057	25.859401132165083
32	24.596886418359766	24.424789155319058	25.18135323601582	25.796971190305356
33	24.625867188916324	24.26852279778061	25.33787493073979	25.76773508256328
34	25.276721674807668	23.54374819668113	25.450989303925297	25.728540824585906
35	24.580417169893703	23.649202454301026	26.163507720119807	25.606872655685464
36	24.342315244395987	23.412249546138153	25.999708915608505	26.245726293857352
37	24.218093703640342	22.321959763966305	26.809638468079022	26.65030806431433
38	23.56813289789832	22.40634870378078	27.604375458011077	26.421142940309828
39	23.403312744644943	21.939847665835117	28.43932805466258	26.21751153485736
40	22.88919132159974	21.62399556734646	29.39045907072585	26.096354040327956
41	22.292468319038704	21.43811009628765	30.047952323440718	26.221469261232922
42	21.85711841772653	21.41410840084874	30.472961068739323	26.255812112685405
43	20.99969614874923	21.050125242889496	30.856349852798115	27.09382875556316
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	45.5
2	60.0
3	138.0
4	216.0
5	216.0
6	312.5
7	409.0
8	402.0
9	395.0
10	604.0
11	813.0
12	813.0
13	1419.0
14	2025.0
15	3215.0
16	4405.0
17	4254.5
18	4104.0
19	4104.0
20	4637.0
21	5170.0
22	5184.5
23	5199.0
24	6204.5
25	7210.0
26	7210.0
27	8373.0
28	9536.0
29	11342.5
30	13149.0
31	15608.5
32	18068.0
33	18068.0
34	20695.0
35	23322.0
36	26689.5
37	30057.0
38	32287.5
39	34518.0
40	34518.0
41	37254.5
42	39991.0
43	42613.5
44	45236.0
45	47260.5
46	49285.0
47	49285.0
48	51051.0
49	52817.0
50	54383.0
51	55949.0
52	56795.0
53	57641.0
54	57641.0
55	56919.5
56	56198.0
57	55324.0
58	54450.0
59	51748.5
60	49047.0
61	49047.0
62	45804.0
63	42561.0
64	39048.5
65	35536.0
66	31251.0
67	26966.0
68	26966.0
69	23666.0
70	20366.0
71	17367.5
72	14369.0
73	11862.0
74	9355.0
75	9355.0
76	7680.5
77	6006.0
78	4866.0
79	3726.0
80	3039.0
81	2352.0
82	2352.0
83	1871.5
84	1391.0
85	1070.0
86	749.0
87	560.5
88	372.0
89	372.0
90	276.0
91	180.0
92	107.5
93	35.0
94	21.5
95	8.0
96	8.0
97	5.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	783278.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.93127356104826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.48588861245902	70.51680538872085
2	6.146043296210337	9.579379628700291
3	1.379167145173828	3.224407562308547
4	0.5581693674670254	1.7399539867788014
5	0.34654589333468383	1.350338140746155
6	0.20279800710446289	0.9482584181575984
7	0.15689352042989924	0.8558838302404876
8	0.10431440119550887	0.6503483308739314
9	0.08500230784770783	0.5961904295580144
>10	0.4945842491235337	7.05822260098418
>50	0.030240243435668723	1.5648488664285254
>100	0.009695625680944684	1.1422252665235524
>500	1.6433263434538029E-4	0.07466277814109508
>1k	4.929979030361408E-4	0.6984747718379634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2280	0.2910843914931863	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1605	0.20490809138007196	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1569	0.20031202204070586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.553371855203389E-4	0.0
3	0.0	0.0	0.0	5.106743710406778E-4	0.0
4	0.0	0.0	0.0	6.383429638008473E-4	0.0
5	0.0	1.2766859276016945E-4	0.0	6.383429638008473E-4	0.0
6	1.2766859276016945E-4	1.2766859276016945E-4	0.0	6.383429638008473E-4	0.0
7	1.2766859276016945E-4	1.2766859276016945E-4	0.0	6.383429638008473E-4	0.0
8	1.2766859276016945E-4	1.2766859276016945E-4	0.0	6.383429638008473E-4	0.0
9	1.2766859276016945E-4	1.2766859276016945E-4	0.0	0.001149017334841525	0.0
10	1.2766859276016945E-4	1.2766859276016945E-4	0.0	0.0015320231131220332	0.0
11	1.2766859276016945E-4	1.2766859276016945E-4	0.0	0.0015320231131220332	0.0
12	1.2766859276016945E-4	1.2766859276016945E-4	0.0	0.0016596917058822028	0.0
13	1.2766859276016945E-4	1.2766859276016945E-4	0.0	0.0020426974841627112	0.0
14	1.2766859276016945E-4	1.2766859276016945E-4	0.0	0.0020426974841627112	0.0
15	1.2766859276016945E-4	1.2766859276016945E-4	0.0	0.0021703660769228804	0.0
16	1.2766859276016945E-4	1.2766859276016945E-4	0.0	0.002553371855203389	0.0
17	1.2766859276016945E-4	1.2766859276016945E-4	0.0	0.0029363776334838973	0.0
18	1.2766859276016945E-4	2.553371855203389E-4	0.0	0.003191714819004236	0.0
19	2.553371855203389E-4	2.553371855203389E-4	0.0	0.003574720597284744	0.0
20	2.553371855203389E-4	2.553371855203389E-4	0.0	0.0039577263755652525	0.0
21	2.553371855203389E-4	2.553371855203389E-4	0.0	0.0045960693393661	0.0
22	5.106743710406778E-4	2.553371855203389E-4	0.0	0.005234412303166947	0.0
23	5.106743710406778E-4	2.553371855203389E-4	0.0	0.007532446972849997	0.0
24	5.106743710406778E-4	2.553371855203389E-4	0.0	0.009958150235293216	0.0
25	5.106743710406778E-4	2.553371855203389E-4	0.0	0.01366053942533813	0.0
26	5.106743710406778E-4	2.553371855203389E-4	0.0	0.01800127157918389	0.0
27	5.106743710406778E-4	2.553371855203389E-4	0.0	0.04991841976922625	0.0
28	6.383429638008473E-4	2.553371855203389E-4	0.0	0.15473433442532536	0.0
29	6.383429638008473E-4	3.830057782805083E-4	0.0	0.3057662796606058	0.0
30	6.383429638008473E-4	3.830057782805083E-4	0.0	0.5160364519366049	0.0
31	6.383429638008473E-4	3.830057782805083E-4	0.0	1.0989712464795385	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	740	0.0	22.75	1
ACGTTAA	45	0.0038247353	20.555557	20
CGCCTAA	45	0.0038247353	20.555557	13
TAAGACT	110	1.7516868E-9	20.181818	4
CTTATAC	950	0.0	18.694736	37
CGTTATT	115	6.397204E-8	17.695652	2
GCGTTAT	95	3.6034216E-6	17.526316	1
CTAGAAC	120	1.03991624E-7	16.958332	3
TGACCCG	90	4.443046E-5	16.444445	13
TAGAACT	160	6.2755134E-10	16.1875	4
CCAATAC	70	0.0025915045	15.857143	3
GTAAGAC	100	1.0926573E-4	14.8	3
GTACGGG	75	0.0041028536	14.8	37
GTATCAA	1140	0.0	14.767544	2
GATTAAT	190	4.5474735E-10	14.605264	1
CGGACCA	130	4.439322E-6	14.230769	9
TCTTATA	1555	0.0	14.038586	37
TCACGAG	80	0.0062962594	13.875	25
TTGGCGT	80	0.0062962594	13.875	23
ATAGCCG	80	0.0062962594	13.875	5
>>END_MODULE