FastQCFastQC Report
Fri 10 Feb 2017
ERR1632313.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632313.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649602
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC250.005494606729.5999981
CCGTTCG250.005494606729.5999989
ACCGTTC358.8652276E-426.428578
ATTACTC656.8962254E-519.9230773
GGTTAGC609.230922E-418.525
TTAGCGA500.007031671218.49999827
ACTTAAT500.007031671218.4999981
GGTATCA3400.017.9558811
TAACGAA953.6015044E-617.52631613
TATGCCG752.0655224E-417.26666833
CTTATAC3650.017.23287837
GTTAATA650.00157893417.0769232
TCCGATA1005.8698806E-616.658
CCGATAA1005.8698806E-616.659
CTATCCT1005.8698806E-616.654
AATTCCG1005.8698806E-616.655
ATAACGA1005.8698806E-616.6512
CGATAAC1005.8698806E-616.6510
GTATTAG1452.9704097E-916.5862061
TCTTATA6250.016.57637