FastQCFastQC Report
Fri 10 Feb 2017
ERR1632312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378807
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29080.7676732478544483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27500.7259633533699219No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16050.42369861169408174No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.2806178344117189No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.13516117706378183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAGTC200.001840378737.02
TCATTTG200.001840378737.022
GACACAT401.5963196E-632.37526
CACATGT401.5963196E-632.37528
ACATGTT401.5963196E-632.37529
GTGACAC401.5963196E-632.37524
AGTGACA502.7126407E-729.623
GGTTATC250.005491749429.617
TGCACTA250.005491749429.612
TTTAACG453.9989354E-628.77777934
TAACGGC453.9989354E-628.77777936
AACGGCC453.9989354E-628.77777937
TTAACGG453.9989354E-628.77777935
GTAAACA405.9297803E-527.7536
GAGGCAC556.2431354E-726.909097
GAGCTGA358.8583765E-426.428576
GTAAGAT358.8583765E-426.428573
TCTCCGT509.070092E-625.90000231
TAAACAG451.3209252E-424.66666837
ATTGCGG751.3675162E-824.66666626
Produced by FastQC (version 0.11.5)