##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632312.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	378807
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.353866216833374	33.0	31.0	34.0	31.0	34.0
2	32.516088667844045	34.0	31.0	34.0	31.0	34.0
3	32.49798974147785	34.0	31.0	34.0	31.0	34.0
4	36.08701000773481	37.0	35.0	37.0	35.0	37.0
5	35.94712610907401	37.0	35.0	37.0	35.0	37.0
6	36.012093229533775	37.0	35.0	37.0	35.0	37.0
7	36.01112703830711	37.0	35.0	37.0	35.0	37.0
8	36.00142024830586	37.0	35.0	37.0	35.0	37.0
9	37.691827236561096	39.0	37.0	39.0	35.0	39.0
10	37.575749656157356	39.0	37.0	39.0	35.0	39.0
11	37.70007154038864	39.0	37.0	39.0	35.0	39.0
12	37.66429870620131	39.0	37.0	39.0	35.0	39.0
13	37.726742114058084	39.0	37.0	39.0	35.0	39.0
14	38.97729450617333	40.0	38.0	41.0	36.0	41.0
15	38.99095581655038	40.0	38.0	41.0	36.0	41.0
16	38.85791973221192	40.0	38.0	41.0	36.0	41.0
17	38.927591095201514	40.0	38.0	41.0	36.0	41.0
18	38.998144173682114	40.0	38.0	41.0	36.0	41.0
19	39.07917752311863	40.0	39.0	41.0	36.0	41.0
20	39.03481456256088	40.0	39.0	41.0	36.0	41.0
21	39.02715102941603	40.0	38.0	41.0	36.0	41.0
22	39.00424226585042	40.0	38.0	41.0	36.0	41.0
23	38.96969432983023	40.0	38.0	41.0	35.0	41.0
24	38.91933095217353	40.0	38.0	41.0	35.0	41.0
25	38.85064953921126	40.0	38.0	41.0	35.0	41.0
26	38.71990221933597	40.0	38.0	41.0	35.0	41.0
27	38.63685729144393	40.0	38.0	41.0	35.0	41.0
28	38.563954731565154	40.0	38.0	41.0	35.0	41.0
29	38.43148885844243	40.0	38.0	41.0	34.0	41.0
30	38.327076849160655	40.0	38.0	41.0	34.0	41.0
31	38.19291618159115	40.0	38.0	41.0	34.0	41.0
32	38.097490278690735	40.0	37.0	41.0	34.0	41.0
33	37.92074592074592	40.0	37.0	41.0	33.0	41.0
34	37.930178164606254	40.0	37.0	41.0	33.0	41.0
35	37.82918214288543	40.0	37.0	41.0	33.0	41.0
36	37.785283798873834	40.0	37.0	41.0	33.0	41.0
37	37.71627504243586	40.0	37.0	41.0	33.0	41.0
38	37.62132959528203	40.0	37.0	41.0	33.0	41.0
39	37.413202501537725	40.0	37.0	41.0	33.0	41.0
40	37.36247482227097	40.0	37.0	41.0	33.0	41.0
41	37.29405475611591	40.0	36.0	41.0	33.0	41.0
42	37.102991760975904	40.0	36.0	41.0	32.0	41.0
43	36.31260773956131	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	1.0
15	0.0
16	0.0
17	1.0
18	5.0
19	13.0
20	24.0
21	38.0
22	110.0
23	194.0
24	335.0
25	560.0
26	933.0
27	1357.0
28	1958.0
29	2894.0
30	3899.0
31	5123.0
32	6864.0
33	9149.0
34	13120.0
35	18568.0
36	29156.0
37	56063.0
38	112177.0
39	116261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17690538981592	21.46739632583347	13.580794441496593	23.774903842854012
2	19.180743756055193	21.140580823480033	35.05294252746121	24.62573289300356
3	19.140089808266477	25.504280543918146	30.239145527933747	25.116484119881626
4	13.945096051551317	18.019730364011224	36.64187831798251	31.393295266454952
5	12.839255874363461	38.08482947780796	35.612594276240934	13.463320371587642
6	30.35952345125618	37.25643929494439	17.010773296164007	15.37326395763542
7	27.557040920574327	30.74705588861874	23.767512215983338	17.92839097482359
8	26.85562832788201	33.362371867468134	20.111824755086364	19.670175049563497
9	25.651057134635845	14.473069399456714	22.437283365935688	37.438590099971755
10	18.57568629935561	27.00715667873086	33.33544522672495	21.08171179518858
11	32.516822550797635	24.25588756279583	22.40560496506136	20.82168492134517
12	20.315094494029942	29.47543208018859	28.486010026213876	21.72346339956759
13	29.77769682186443	20.66118102358193	25.727877256756077	23.83324489779756
14	22.399797258234404	22.46869778013606	27.002140931925755	28.12936402970378
15	25.59614790645368	28.208296045215636	23.145031638802873	23.050524409527807
16	23.458383820784725	25.574764985863514	27.56311261407524	23.403738579276517
17	21.33989076231432	28.449579865208403	27.441678744057	22.768850628420278
18	21.073528208296043	26.737098311277247	28.217799565477936	23.971573914948774
19	22.439659246001263	26.305480099364587	30.530850802651482	20.72400985198267
20	21.805035281818974	26.728122764362855	30.165493245900947	21.301348707917224
21	22.59910719706869	26.688260776595996	28.836584329223058	21.87604769711225
22	23.585097424282022	25.422972648340714	28.73970121988242	22.252228707494844
23	22.319545309352783	26.763760965346467	29.260282940917143	21.656410784383603
24	21.100718835713174	27.379905862352068	28.0137378665125	23.505637435422262
25	22.519383221534977	26.67770130963789	29.361389837041024	21.441525631786106
26	21.615756836594887	27.296486073383015	29.000520053747685	22.08723703627441
27	21.1814987579427	27.42689549031565	28.651265684108267	22.740340067633387
28	21.548968208084858	26.588737800515833	30.64410108577719	21.218192905622125
29	22.501432127706195	26.477599410781743	29.514238121259638	21.506730340252425
30	21.70709622578252	27.433759143838422	28.587116922337763	22.272027708041296
31	22.4533865530468	26.87357942171079	29.135681230811468	21.537352794430937
32	21.66644227799381	26.7278587776889	29.13647319083333	22.469225753483965
33	20.573801434503586	27.769022219758348	29.517669948021023	22.139506397717042
34	23.033893249068786	25.978664597011143	28.690335711853265	22.297106442066806
35	21.582230529002896	27.290942353230008	29.559643829179507	21.56718328858759
36	22.3311607230067	26.864867861470355	27.52853035978744	23.275441055735506
37	22.141618291108664	25.38073478050828	29.307536555554677	23.17011037282838
38	21.17674699781155	26.83899716742299	29.456953013011905	22.52730282175356
39	20.479822178576427	26.476015490738025	30.15994952574794	22.884212804937608
40	21.173579157724117	25.770643097936418	31.174978287096067	21.8807994572434
41	20.37264358895163	26.031989905149587	30.443471213573137	23.151895292325644
42	19.73696367807353	26.520893225309987	30.35107587768969	23.39106721892679
43	20.38821880271484	25.800737578767023	30.911783573165224	22.89926004535291
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	41.0
2	63.0
3	180.5
4	298.0
5	298.0
6	405.0
7	512.0
8	507.0
9	502.0
10	756.0
11	1010.0
12	1010.0
13	1990.5
14	2971.0
15	4253.5
16	5536.0
17	4723.5
18	3911.0
19	3911.0
20	4501.0
21	5091.0
22	3621.5
23	2152.0
24	2078.5
25	2005.0
26	2005.0
27	2739.0
28	3473.0
29	5005.5
30	6538.0
31	8645.5
32	10753.0
33	10753.0
34	14058.5
35	17364.0
36	19152.0
37	20940.0
38	22137.0
39	23334.0
40	23334.0
41	26906.0
42	30478.0
43	32874.0
44	35270.0
45	37304.0
46	39338.0
47	39338.0
48	39030.0
49	38722.0
50	36398.5
51	34075.0
52	31274.5
53	28474.0
54	28474.0
55	25329.5
56	22185.0
57	18093.0
58	14001.0
59	12800.0
60	11599.0
61	11599.0
62	9821.0
63	8043.0
64	6383.0
65	4723.0
66	3846.0
67	2969.0
68	2969.0
69	1961.0
70	953.0
71	773.5
72	594.0
73	499.0
74	404.0
75	404.0
76	320.5
77	237.0
78	183.5
79	130.0
80	91.5
81	53.0
82	53.0
83	48.5
84	44.0
85	37.5
86	31.0
87	19.5
88	8.0
89	8.0
90	6.0
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	378807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.477021255968364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62199119179462	18.96336983179925
2	9.361998848644008	4.9575568502779745
3	4.06201169098032	3.2264990965223372
4	2.4629998036775205	2.6085159262166244
5	1.6959997924808412	2.245251127781658
6	1.2839998421028624	2.0397894667210505
7	0.9969998773872342	1.8478310862045753
8	0.7669999056729297	1.624629824466231
9	0.7489999078866014	1.7848157833648701
>10	5.879999276866778	31.991916078948808
>50	0.8029999012455821	14.602340197056215
>100	0.30999996187562945	11.767445879820247
>500	9.999998770181594E-4	0.13556233215885086
>1k	0.0039999995080726375	2.2044765186613127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2908	0.7676732478544483	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2750	0.7259633533699219	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1605	0.42369861169408174	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1063	0.2806178344117189	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	512	0.13516117706378183	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	5.279733479053977E-4	0.0
16	0.0	0.0	0.0	0.003695813435337784	0.0
17	0.0	0.0	0.0	0.006071693500912074	2.6398667395269884E-4
18	0.0	0.0	0.0	0.006071693500912074	2.6398667395269884E-4
19	0.0	0.0	0.0	0.007919600218580966	2.6398667395269884E-4
20	0.0	0.0	0.0	0.008975546914391761	2.6398667395269884E-4
21	0.0	0.0	0.0	0.010031493610202558	2.6398667395269884E-4
22	0.0	0.0	0.0	0.012407373675776847	2.6398667395269884E-4
23	0.0	0.0	0.0	0.015311227089256535	2.6398667395269884E-4
24	0.0	0.0	0.0	0.022438867285979405	2.6398667395269884E-4
25	0.0	0.0	0.0	0.02534272069945909	2.6398667395269884E-4
26	0.0	0.0	0.0	0.028510560786891476	2.6398667395269884E-4
27	0.0	0.0	0.0	0.03854205439709404	2.6398667395269884E-4
28	0.0	0.0	0.0	0.05781308159564105	2.6398667395269884E-4
29	0.0	0.0	0.0	0.09925898940621478	2.6398667395269884E-4
30	0.0	0.0	0.0	0.18320675172317302	2.6398667395269884E-4
31	0.0	0.0	0.0	0.4134031314099264	2.6398667395269884E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCAGTC	20	0.0018403787	37.0	2
TCATTTG	20	0.0018403787	37.0	22
GACACAT	40	1.5963196E-6	32.375	26
CACATGT	40	1.5963196E-6	32.375	28
ACATGTT	40	1.5963196E-6	32.375	29
GTGACAC	40	1.5963196E-6	32.375	24
AGTGACA	50	2.7126407E-7	29.6	23
GGTTATC	25	0.0054917494	29.6	17
TGCACTA	25	0.0054917494	29.6	12
TTTAACG	45	3.9989354E-6	28.777779	34
TAACGGC	45	3.9989354E-6	28.777779	36
AACGGCC	45	3.9989354E-6	28.777779	37
TTAACGG	45	3.9989354E-6	28.777779	35
GTAAACA	40	5.9297803E-5	27.75	36
GAGGCAC	55	6.2431354E-7	26.90909	7
GAGCTGA	35	8.8583765E-4	26.42857	6
GTAAGAT	35	8.8583765E-4	26.42857	3
TCTCCGT	50	9.070092E-6	25.900002	31
TAAACAG	45	1.3209252E-4	24.666668	37
ATTGCGG	75	1.3675162E-8	24.666666	26
>>END_MODULE