FastQCFastQC Report
Fri 10 Feb 2017
ERR1632311.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632311.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722031
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12380.17146078215478283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8350.11564600411893672No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7630.10567413310508827No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCC250.005495005829.63
GGTATCA5600.021.8035721
ACTATTC555.140548E-420.1818183
GGATAGA555.140548E-420.1818181
CGTTATT759.257947E-619.7333342
CTTATAC6200.018.79838837
CGACCGT500.007032409318.56
TATTATG609.232282E-418.52
ACCCGTT902.1499618E-618.530
TATACAC2200.017.6590937
CTAGACC650.001579165117.0769234
CGGGTAA1107.7977165E-716.81818224
GCGGTAT1005.8716996E-616.65000224
TAACCCG1005.8716996E-616.65000228
TAATACT803.380387E-416.18754
TAGGACA1151.2405544E-616.0869564
GTAACCC1151.2405544E-616.08695627
TTGATTA1059.333395E-615.85714436
CGGTATC1059.333395E-615.85714425
GTTATTC700.002591241615.8571433
Produced by FastQC (version 0.11.5)