##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632311.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	722031
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.254886563042305	33.0	31.0	34.0	30.0	34.0
2	32.418824122509974	34.0	31.0	34.0	31.0	34.0
3	32.43937310170893	34.0	31.0	34.0	31.0	34.0
4	36.05980906636972	37.0	35.0	37.0	35.0	37.0
5	35.85535801094413	37.0	35.0	37.0	35.0	37.0
6	35.85735100016481	37.0	35.0	37.0	35.0	37.0
7	35.84765058563968	37.0	35.0	37.0	35.0	37.0
8	35.816755790263855	37.0	35.0	37.0	35.0	37.0
9	37.53547008369447	39.0	37.0	39.0	35.0	39.0
10	37.393454020672245	39.0	37.0	39.0	35.0	39.0
11	37.531485490235184	39.0	37.0	39.0	35.0	39.0
12	37.45797479609601	39.0	37.0	39.0	35.0	39.0
13	37.513054148644585	39.0	37.0	39.0	35.0	39.0
14	38.76239524341753	40.0	38.0	41.0	36.0	41.0
15	38.76006431856804	40.0	38.0	41.0	35.0	41.0
16	38.64563155875579	40.0	38.0	41.0	35.0	41.0
17	38.69499370525642	40.0	38.0	41.0	35.0	41.0
18	38.70213882783426	40.0	38.0	41.0	35.0	41.0
19	38.74880995414324	40.0	38.0	41.0	35.0	41.0
20	38.72199116104433	40.0	38.0	41.0	35.0	41.0
21	38.6887017316431	40.0	38.0	41.0	35.0	41.0
22	38.65641918421785	40.0	38.0	41.0	35.0	41.0
23	38.60322340730523	40.0	38.0	41.0	35.0	41.0
24	38.53589527319464	40.0	38.0	41.0	34.0	41.0
25	38.46446897709378	40.0	38.0	41.0	34.0	41.0
26	38.35513849128362	40.0	38.0	41.0	34.0	41.0
27	38.26538334226647	40.0	38.0	41.0	34.0	41.0
28	38.17013119935294	40.0	38.0	41.0	34.0	41.0
29	38.00911595208516	40.0	38.0	41.0	33.0	41.0
30	37.95800457321084	40.0	37.0	41.0	33.0	41.0
31	37.86118047563055	40.0	37.0	41.0	33.0	41.0
32	37.776033162011046	40.0	37.0	41.0	33.0	41.0
33	37.61648599575364	40.0	37.0	41.0	33.0	41.0
34	37.60462916412176	40.0	37.0	41.0	33.0	41.0
35	37.569299656108946	40.0	37.0	41.0	33.0	41.0
36	37.54242407874454	39.0	37.0	41.0	33.0	41.0
37	37.50219034916783	39.0	37.0	41.0	33.0	41.0
38	37.42293336435693	39.0	36.0	41.0	32.0	41.0
39	37.231296163184126	39.0	36.0	41.0	32.0	41.0
40	37.228803195430665	39.0	36.0	41.0	32.0	41.0
41	37.21768040430397	39.0	36.0	41.0	32.0	41.0
42	37.08030680123153	39.0	36.0	40.0	31.0	41.0
43	36.13911868049987	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	0.0
15	0.0
16	4.0
17	5.0
18	22.0
19	37.0
20	77.0
21	194.0
22	346.0
23	593.0
24	901.0
25	1371.0
26	2045.0
27	3025.0
28	4323.0
29	6095.0
30	8197.0
31	10924.0
32	14722.0
33	20208.0
34	28492.0
35	41656.0
36	64905.0
37	113352.0
38	218920.0
39	181614.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52486250590349	17.253829821711257	11.90710648157766	27.314201190807598
2	19.67491700494854	19.657189234257256	32.66563347003106	28.002260290763136
3	20.429870739621983	20.833177522848743	28.890310803829756	29.846640933699526
4	15.447397687910907	14.923181968641236	32.76341320525019	36.866007138197666
5	16.755928762061462	34.101028903191136	32.68045277834331	16.46258955640409
6	36.92666935353191	33.25258887776287	14.756568623784853	15.064173144920371
7	31.757223720311178	28.891280291289434	19.55747052411877	19.794025464280622
8	29.646788018796975	31.571359124469723	18.66609605404754	20.11575680268576
9	27.833015479944766	13.282809186863167	17.65547462643571	41.228700706756364
10	17.92291466709878	25.45832519656358	31.34048261085743	25.27827752548021
11	39.34277060126227	19.79527194815735	19.715773976463613	21.14618347411676
12	23.870443235816744	23.48804968207736	27.41267341706935	25.228833665036543
13	32.92268614505471	17.640239823497883	22.37078463390076	27.066289397546644
14	24.21627326250535	18.30184576562502	23.20011190655249	34.28176906531714
15	27.149526820870573	25.303345701223357	20.419621872191083	27.12750560571499
16	27.271405244373163	23.930689956525413	21.97551074676849	26.822394052332932
17	25.909967854565803	24.351586012234932	23.326006778102325	26.41243935509694
18	26.353993111099104	23.13252478079196	24.076944064728522	26.43653804338041
19	27.03332682391753	23.211053265026017	23.813797468529742	25.941822442526707
20	27.216005960962896	22.996381041811222	23.405504749796062	26.38210824742982
21	27.936750638130498	23.067846117410472	22.839878066177214	26.15552517828182
22	27.995335380336854	22.943613224362945	22.87907305918998	26.181978336110223
23	27.151604293998453	22.863838256252155	23.04388592733553	26.940671522413854
24	26.79829536404947	23.18917054807896	23.065214651448485	26.94731943642309
25	26.80577426730985	22.499864964246687	23.391931925360545	27.302428843082915
26	26.698576653910983	23.010646357289367	23.452178646069214	26.838598342730435
27	26.716304424602267	22.934610840808777	23.28307233345937	27.06601240112959
28	26.599827431232175	23.063829669363226	23.56782465018815	26.768518249216445
29	26.380030774301932	23.416307610061065	23.628486865522394	26.575174750114606
30	26.20898548677273	23.55411332754411	23.389161961190034	26.847739224493132
31	26.580991674872685	23.36298579977868	23.106071622963555	26.949950902385076
32	25.90068847459458	23.29221321522206	23.578489012244628	27.228609297938732
33	25.80470921608629	23.088482350480795	24.28759983989607	26.819208593536842
34	26.35800955914635	22.75774862852149	24.015312361934598	26.86892945039756
35	26.02048942496929	22.84541799451824	24.569443694245816	26.564648886266657
36	25.786565950769425	22.90843467939742	24.362665868916984	26.942333500916167
37	25.668842473522606	22.141570098790773	24.79020983863574	27.39937758905089
38	25.062774313014263	22.031602521221387	25.71911732321742	27.186505842546925
39	25.04753951007644	21.55406069822487	26.11563769422642	27.28276209747227
40	24.739796490732395	21.38343090532124	27.06601240112959	26.810760202816773
41	24.022237272360883	21.30504091929571	27.62582215999036	27.04689964835305
42	23.764076611669026	21.165296226893304	28.140066008246183	26.930561153191483
43	22.96286447534801	20.667672163660562	28.602096031887825	27.7673673291036
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	10.0
2	11.0
3	56.0
4	101.0
5	101.0
6	145.0
7	189.0
8	189.5
9	190.0
10	261.5
11	333.0
12	333.0
13	626.0
14	919.0
15	1599.0
16	2279.0
17	2159.0
18	2039.0
19	2039.0
20	2275.0
21	2511.0
22	2456.5
23	2402.0
24	3002.5
25	3603.0
26	3603.0
27	4547.0
28	5491.0
29	6781.0
30	8071.0
31	9712.0
32	11353.0
33	11353.0
34	13789.0
35	16225.0
36	18765.0
37	21305.0
38	24358.0
39	27411.0
40	27411.0
41	30376.5
42	33342.0
43	35943.0
44	38544.0
45	41335.5
46	44127.0
47	44127.0
48	47463.5
49	50800.0
50	52859.0
51	54918.0
52	55955.5
53	56993.0
54	56993.0
55	57095.0
56	57197.0
57	56773.5
58	56350.0
59	54895.0
60	53440.0
61	53440.0
62	49639.5
63	45839.0
64	41279.5
65	36720.0
66	32735.5
67	28751.0
68	28751.0
69	24757.5
70	20764.0
71	17935.5
72	15107.0
73	12320.5
74	9534.0
75	9534.0
76	7803.5
77	6073.0
78	4991.5
79	3910.0
80	3182.5
81	2455.0
82	2455.0
83	1921.0
84	1387.0
85	1074.5
86	762.0
87	563.0
88	364.0
89	364.0
90	268.5
91	173.0
92	101.5
93	30.0
94	17.5
95	5.0
96	5.0
97	3.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	722031.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.99135853475964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.70090806737586	67.40502507306712
2	7.017909104084385	10.666008937856606
3	1.8042649172907015	4.113256266645784
4	0.8118181815846001	2.4676466600732776
5	0.4449806530464727	1.6907342173342994
6	0.2686522311366022	1.2249148810478794
7	0.174502888488237	0.9282498095122558
8	0.12454965814222392	0.7571758181813987
9	0.09487697283689298	0.6488847053586887
>10	0.5158394802182763	6.973109819813297
>50	0.0305421894049862	1.5961551152696085
>100	0.010607017554349642	1.1347121137414646
>500	3.6575922422446685E-4	0.22207837736004163
>1k	1.8287961211223343E-4	0.17204820473825505
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1238	0.17146078215478283	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	835	0.11564600411893672	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	763	0.10567413310508827	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3849820852567272E-4	0.0	1.3849820852567272E-4	0.0
3	0.0	1.3849820852567272E-4	0.0	1.3849820852567272E-4	0.0
4	0.0	2.7699641705134545E-4	0.0	1.3849820852567272E-4	0.0
5	1.3849820852567272E-4	4.1549462557701817E-4	0.0	2.7699641705134545E-4	0.0
6	1.3849820852567272E-4	4.1549462557701817E-4	0.0	2.7699641705134545E-4	0.0
7	1.3849820852567272E-4	4.1549462557701817E-4	0.0	6.924910426283636E-4	0.0
8	1.3849820852567272E-4	4.1549462557701817E-4	0.0	8.309892511540363E-4	0.0
9	1.3849820852567272E-4	4.1549462557701817E-4	0.0	9.69487459679709E-4	0.0
10	1.3849820852567272E-4	4.1549462557701817E-4	0.0	0.0018004767108337455	0.0
11	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.0018004767108337455	0.0
12	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.0018004767108337455	0.0
13	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.001938974919359418	0.0
14	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.001938974919359418	0.0
15	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.001938974919359418	0.0
16	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.0022159713364107636	0.0
17	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.0023544695449364363	0.0
18	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.0023544695449364363	1.3849820852567272E-4
19	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.002492967753462109	1.3849820852567272E-4
20	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.002631465961987782	1.3849820852567272E-4
21	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.0031854587960904726	1.3849820852567272E-4
22	1.3849820852567272E-4	5.539928341026909E-4	0.0	0.003600953421667491	1.3849820852567272E-4
23	1.3849820852567272E-4	8.309892511540363E-4	0.0	0.0045704408813471995	1.3849820852567272E-4
24	1.3849820852567272E-4	8.309892511540363E-4	0.0	0.006232419383655273	1.3849820852567272E-4
25	1.3849820852567272E-4	8.309892511540363E-4	0.0	0.006924910426283636	1.3849820852567272E-4
26	1.3849820852567272E-4	8.309892511540363E-4	0.0	0.00969487459679709	1.3849820852567272E-4
27	1.3849820852567272E-4	8.309892511540363E-4	0.0	0.02506817574314676	1.3849820852567272E-4
28	1.3849820852567272E-4	0.0012464838767310546	0.0	0.08905434808200756	1.3849820852567272E-4
29	1.3849820852567272E-4	0.0012464838767310546	0.0	0.18849606180344058	1.3849820852567272E-4
30	1.3849820852567272E-4	0.0012464838767310546	0.0	0.3386281198452698	1.3849820852567272E-4
31	1.3849820852567272E-4	0.0012464838767310546	0.0	0.7366719711480532	1.3849820852567272E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCC	25	0.0054950058	29.6	3
GGTATCA	560	0.0	21.803572	1
ACTATTC	55	5.140548E-4	20.181818	3
GGATAGA	55	5.140548E-4	20.181818	1
CGTTATT	75	9.257947E-6	19.733334	2
CTTATAC	620	0.0	18.798388	37
CGACCGT	50	0.0070324093	18.5	6
TATTATG	60	9.232282E-4	18.5	2
ACCCGTT	90	2.1499618E-6	18.5	30
TATACAC	220	0.0	17.65909	37
CTAGACC	65	0.0015791651	17.076923	4
CGGGTAA	110	7.7977165E-7	16.818182	24
GCGGTAT	100	5.8716996E-6	16.650002	24
TAACCCG	100	5.8716996E-6	16.650002	28
TAATACT	80	3.380387E-4	16.1875	4
TAGGACA	115	1.2405544E-6	16.086956	4
GTAACCC	115	1.2405544E-6	16.086956	27
TTGATTA	105	9.333395E-6	15.857144	36
CGGTATC	105	9.333395E-6	15.857144	25
GTTATTC	70	0.0025912416	15.857143	3
>>END_MODULE