FastQCFastQC Report
Fri 10 Feb 2017
ERR1632310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11364
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1441.2671594508975714No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT1391.2231608588525167TruSeq Adapter, Index 7 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1100.9679690249912002No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT890.783174938401971No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT710.6247800070397747No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC670.5895811334037311No Hit
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC610.5367828229496656No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG590.5191833861316438No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC380.33438929954241464RNA PCR Primer, Index 7 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.2815909890883492No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC190.16719464977120732No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG180.15839493136219643No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA170.14959521295318548No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC160.1407954945441746No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTC160.1407954945441746No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC160.1407954945441746No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTA140.12319605772615277No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.11439633931714185No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA130.11439633931714185No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.10559662090813093No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAA120.10559662090813093No Hit
GTTTTTAATAAACAGTCGCAGCCACCGATTTTTTGCAACCCAT120.10559662090813093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGATT200.0017662337.015
TCCTCGC200.0017662337.021
CAGTCGG200.0017662337.010
GCCTTAT200.0017662337.026
GGGCAGT200.0017662337.07
CGCCTTA200.0017662337.025
TCGGTGA200.0017662337.013
TTATTGA200.0017662337.029
TGATTCC200.0017662337.017
TATTGAT200.0017662337.030
TTCCTCG200.0017662337.020
TCGCCTT200.0017662337.024
GCAGTCG200.0017662337.09
CTTATTG200.0017662337.028
AGTCGGT200.0017662337.011
CGGTGAT200.0017662337.014
ATTGATA200.0017662337.031
GTGATTC200.0017662337.016
GATTCCT200.0017662337.018
CCTTATT200.0017662337.027
Produced by FastQC (version 0.11.5)