FastQCFastQC Report
Fri 10 Feb 2017
ERR1632309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735613
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3500.024.3142851
ACTATAC551.9014518E-523.5454543
TAGCACA753.7369864E-722.1999994
TACCCCG450.00382451820.5555555
TAAGCGT450.00382451820.5555554
GTATCAA4000.019.88752
TACGCTA500.00703253318.59
GTACTAT701.21844045E-418.51
TACTATA701.21844045E-418.52
GTATACA701.21844045E-418.51
CGCGTAA801.615381E-518.510
GTCTAGA1156.395567E-817.6956521
ACTGATC1005.872005E-616.6500028
CGCGCTT700.002591304515.85714212
TCTAGAC1059.333875E-615.8571423
ACCCGGT700.002591304515.85714212
GTCGCGT957.054152E-515.5789488
CTATACT957.054152E-515.5789484
CGTAACT957.054152E-515.57894812
TTACACT957.054152E-515.5789484
Produced by FastQC (version 0.11.5)