##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632309.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	735613
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45197270847579	31.0	31.0	34.0	30.0	34.0
2	31.63418672590071	31.0	31.0	34.0	30.0	34.0
3	31.66894549171915	31.0	31.0	34.0	30.0	34.0
4	35.50264473303218	37.0	35.0	37.0	33.0	37.0
5	34.7368670754867	35.0	35.0	37.0	31.0	37.0
6	34.67054415840938	35.0	35.0	37.0	31.0	37.0
7	34.678223468046376	35.0	35.0	37.0	32.0	37.0
8	34.61984766446488	35.0	35.0	37.0	31.0	37.0
9	36.227969054380495	37.0	35.0	39.0	32.0	39.0
10	35.915644503291816	37.0	35.0	39.0	31.0	39.0
11	36.12011614802892	37.0	35.0	39.0	32.0	39.0
12	36.025153171572555	37.0	35.0	39.0	32.0	39.0
13	36.13135167540541	37.0	35.0	39.0	32.0	39.0
14	37.13158957223431	38.0	36.0	40.0	32.0	41.0
15	37.07609979704002	38.0	36.0	40.0	32.0	41.0
16	36.96127311507546	38.0	36.0	40.0	32.0	41.0
17	36.97094124220208	38.0	36.0	40.0	31.0	41.0
18	36.98749070503104	38.0	36.0	40.0	32.0	41.0
19	37.04100253801931	39.0	36.0	40.0	31.0	41.0
20	36.9947920985627	39.0	36.0	40.0	31.0	41.0
21	36.950706417640795	38.0	36.0	40.0	31.0	41.0
22	36.923663665541525	38.0	36.0	40.0	31.0	41.0
23	36.85987876777599	38.0	36.0	40.0	31.0	41.0
24	36.767128911533646	38.0	36.0	40.0	31.0	41.0
25	36.65811099042567	38.0	36.0	40.0	31.0	41.0
26	36.50138591895467	38.0	35.0	40.0	30.0	41.0
27	36.37718066428951	38.0	35.0	40.0	30.0	41.0
28	36.26357745173073	38.0	35.0	40.0	30.0	41.0
29	36.09541294131561	38.0	35.0	40.0	30.0	41.0
30	35.98992948737991	38.0	35.0	40.0	29.0	41.0
31	35.884682570862665	38.0	34.0	40.0	29.0	41.0
32	35.75916548511242	38.0	34.0	40.0	29.0	41.0
33	35.58537709366202	38.0	34.0	40.0	28.0	41.0
34	35.57460648465973	38.0	34.0	40.0	28.0	41.0
35	35.53581706685445	38.0	34.0	40.0	28.0	41.0
36	35.490713187504845	38.0	34.0	40.0	28.0	41.0
37	35.45719828224895	38.0	34.0	40.0	27.0	41.0
38	35.363676280870514	38.0	34.0	40.0	27.0	41.0
39	35.16252975409624	38.0	33.0	40.0	27.0	41.0
40	35.14278703611818	38.0	33.0	40.0	27.0	41.0
41	35.117018051611375	38.0	33.0	40.0	27.0	41.0
42	34.99107954862136	38.0	33.0	40.0	27.0	41.0
43	34.02012743113566	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	0.0
13	1.0
14	6.0
15	12.0
16	28.0
17	62.0
18	91.0
19	180.0
20	319.0
21	585.0
22	967.0
23	1589.0
24	2386.0
25	3650.0
26	5245.0
27	7468.0
28	10492.0
29	14168.0
30	18999.0
31	25586.0
32	34262.0
33	45863.0
34	61760.0
35	83255.0
36	108471.0
37	132701.0
38	128887.0
39	48575.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.850112763096895	18.345108093522	11.759987928435196	27.044791214945903
2	21.21427979114018	20.389933293729175	31.530981643880686	26.864805271249963
3	21.635153266731283	21.24160394120278	28.254530575180155	28.868712216885783
4	16.82419968108231	15.382001133748316	31.80218402883038	35.991615156338995
5	17.22400229468484	33.913484400085366	32.227543558909375	16.634969746320415
6	37.301135243667524	32.427648777278264	14.614749875274091	15.656466103780112
7	31.354258285266845	28.186424111591286	20.061771610887792	20.39754599225408
8	28.6717336425539	31.039962589024395	19.02780402195176	21.260499746469954
9	27.71402897991199	13.223393278802847	18.515985987197073	40.546591754088084
10	18.116455255684716	25.428180306764563	31.790764981043022	24.664599456507702
11	38.7096204118198	19.906798819487964	19.648374892776502	21.735205875915735
12	23.504070754595148	23.50175975682866	27.48333702639839	25.510832462177802
13	32.57541669328845	18.182250721507096	22.379294547540624	26.863038037663827
14	24.6864859647668	19.111815587815876	22.76101700214651	33.44068144527081
15	27.43480607330213	25.950057978855728	20.22041481050498	26.394721137337157
16	27.41998849938759	23.994681986316174	21.84762912020315	26.73770039409309
17	25.87909675332002	24.69042825507434	23.1984752852383	26.231999706367343
18	26.23322317577313	23.27025215704453	24.019015433386848	26.477509233795487
19	26.64879495060582	23.618804996649054	23.732451710342257	25.999948342402867
20	26.813283615161776	23.541998306174577	23.903465545062417	25.741252533601227
21	27.382196888853244	23.17726848220464	23.470765198548694	25.969769430393423
22	27.29397115059141	23.297440366062048	23.076943990930012	26.33164449241653
23	26.433600276232205	23.236402836817728	23.291730842168366	27.0382660447817
24	26.541537466031734	23.532210550928273	23.16693696277798	26.759315020262015
25	27.013252892485585	23.26318322269998	23.272970977946283	26.45059290686815
26	27.124044844231953	23.1820264187827	22.98219308250398	26.711735654481366
27	26.91850198405955	23.238306011448955	23.327619278071484	26.515572726420007
28	26.096738366505214	23.445344223117317	23.653605904191473	26.804311506185996
29	26.18822668984915	23.489525062770777	23.60670624363626	26.715542003743813
30	25.83097362335902	23.637157037735875	23.988564639287233	26.54330469961787
31	26.839112413728415	23.44140193280978	23.26141598911384	26.458069664347967
32	26.169194943536887	23.394910095389832	23.739248762596638	26.696646198476643
33	26.040050950703698	23.11459966041927	23.922089468239413	26.923259920637616
34	26.519515016727546	22.75870600438002	23.763446268622225	26.95833271027021
35	26.107885532202395	22.91666949877177	24.386735960348716	26.58870900867712
36	26.049023059679477	22.809276073152596	24.324474961698613	26.817225905469318
37	26.07566750451664	22.288621870467214	24.93716125190827	26.698549373107873
38	25.523474979370945	22.08552594910639	25.645414096814495	26.74558497470817
39	25.45346534115085	21.978812228712652	26.249943924318902	26.317778505817596
40	25.35395649614675	21.79733093352075	26.397983722419262	26.450728847913236
41	24.712450704378526	21.547879115785065	27.176382146590665	26.56328803324574
42	24.553399681626072	21.650650545871265	27.125948018863177	26.670001753639482
43	23.498497171746557	21.593691247979578	27.407074100104268	27.5007374801696
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	21.0
2	25.0
3	47.5
4	70.0
5	70.0
6	93.5
7	117.0
8	118.0
9	119.0
10	153.0
11	187.0
12	187.0
13	375.0
14	563.0
15	777.0
16	991.0
17	1053.5
18	1116.0
19	1116.0
20	1461.0
21	1806.0
22	1976.0
23	2146.0
24	2750.0
25	3354.0
26	3354.0
27	4327.0
28	5300.0
29	6502.0
30	7704.0
31	9432.0
32	11160.0
33	11160.0
34	13743.0
35	16326.0
36	19016.5
37	21707.0
38	24723.0
39	27739.0
40	27739.0
41	30859.0
42	33979.0
43	37124.0
44	40269.0
45	44174.0
46	48079.0
47	48079.0
48	51019.0
49	53959.0
50	56490.5
51	59022.0
52	60713.0
53	62404.0
54	62404.0
55	62176.5
56	61949.0
57	59811.5
58	57674.0
59	56387.5
60	55101.0
61	55101.0
62	51216.0
63	47331.0
64	41710.0
65	36089.0
66	32285.0
67	28481.0
68	28481.0
69	23639.0
70	18797.0
71	15976.0
72	13155.0
73	10290.0
74	7425.0
75	7425.0
76	5892.0
77	4359.0
78	3596.0
79	2833.0
80	2428.0
81	2023.0
82	2023.0
83	1518.5
84	1014.0
85	841.5
86	669.0
87	487.0
88	305.0
89	305.0
90	254.5
91	204.0
92	115.5
93	27.0
94	21.0
95	15.0
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	735613.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.67824689751063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.180346051548	56.386719465324376
2	6.744046474903745	8.723862059842213
3	2.1325140578305457	4.137818122343289
4	1.0487322965924688	2.7132066563360415
5	0.6228689803076272	2.01430368465687
6	0.3897303597557228	1.512424585904177
7	0.2858114954940253	1.294005053019652
8	0.21792731617311245	1.1276125408925142
9	0.1662417399291554	0.9677001869828697
>10	1.0800948742049925	13.390827662830882
>50	0.096598526333557	4.202573230501751
>100	0.03508782692708751	3.528946751365313
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	1.3594104508756643E-4	0.0	0.0	0.0
11	0.0	1.3594104508756643E-4	0.0	0.0	0.0
12	0.0	1.3594104508756643E-4	0.0	0.0	0.0
13	0.0	1.3594104508756643E-4	0.0	0.0	0.0
14	0.0	1.3594104508756643E-4	0.0	0.0	0.0
15	0.0	1.3594104508756643E-4	0.0	1.3594104508756643E-4	0.0
16	0.0	1.3594104508756643E-4	0.0	1.3594104508756643E-4	0.0
17	0.0	1.3594104508756643E-4	0.0	6.797052254378321E-4	0.0
18	0.0	1.3594104508756643E-4	0.0	8.156462705253986E-4	0.0
19	0.0	1.3594104508756643E-4	0.0	0.0012234694057880978	0.0
20	0.0	1.3594104508756643E-4	0.0	0.0013594104508756642	0.0
21	0.0	1.3594104508756643E-4	0.0	0.00190317463122593	0.0
22	0.0	1.3594104508756643E-4	0.0	0.002854761946838895	0.0
23	0.0	1.3594104508756643E-4	0.0	0.004350113442802126	0.0
24	0.0	1.3594104508756643E-4	0.0	0.007068934344553454	0.0
25	0.0	1.3594104508756643E-4	0.0	0.008836167930691818	0.0
26	0.0	1.3594104508756643E-4	0.0	0.01332222241858151	0.0
27	0.0	1.3594104508756643E-4	0.0	0.03303367395627864	0.0
28	0.0	1.3594104508756643E-4	0.0	0.07735045465482529	0.0
29	0.0	2.7188209017513286E-4	0.0	0.13947551225984314	0.0
30	0.0	2.7188209017513286E-4	0.0	0.22539025275518512	0.0
31	0.0	2.7188209017513286E-4	0.0	0.4316128181530234	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	350	0.0	24.314285	1
ACTATAC	55	1.9014518E-5	23.545454	3
TAGCACA	75	3.7369864E-7	22.199999	4
TACCCCG	45	0.003824518	20.555555	5
TAAGCGT	45	0.003824518	20.555555	4
GTATCAA	400	0.0	19.8875	2
TACGCTA	50	0.007032533	18.5	9
GTACTAT	70	1.21844045E-4	18.5	1
TACTATA	70	1.21844045E-4	18.5	2
GTATACA	70	1.21844045E-4	18.5	1
CGCGTAA	80	1.615381E-5	18.5	10
GTCTAGA	115	6.395567E-8	17.695652	1
ACTGATC	100	5.872005E-6	16.650002	8
CGCGCTT	70	0.0025913045	15.857142	12
TCTAGAC	105	9.333875E-6	15.857142	3
ACCCGGT	70	0.0025913045	15.857142	12
GTCGCGT	95	7.054152E-5	15.578948	8
CTATACT	95	7.054152E-5	15.578948	4
CGTAACT	95	7.054152E-5	15.578948	12
TTACACT	95	7.054152E-5	15.578948	4
>>END_MODULE