FastQCFastQC Report
Fri 10 Feb 2017
ERR1632308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225570
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.13299640909695437No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT2760.12235669636919803RNA PCR Primer, Index 47 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2710.12014008955091544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACT250.00548709729.64
ATTGTGT358.8472164E-426.4285726
GCCGTCT451.3186112E-424.66666636
CTTATAC1150.024.13043237
GTCTCGC502.692782E-422.21
TATGCCG502.692782E-422.233
CCGTCTT502.692782E-422.237
GGTGTGC450.003816424220.5555556
CGTATGC555.1254977E-420.1818231
GTATGCC555.1254977E-420.1818232
GGTATCA2250.018.9111121
CTCGTAT609.2054764E-418.529
CTACTCG500.007017791318.514
TAGCGCG500.007017791318.54
CGCGCGT500.007017791318.57
ATGCCGT609.2054764E-418.534
TGCCGTC609.2054764E-418.535
GTTCTAG852.7074366E-517.4117641
TCTCGTA650.001574617217.07692328
TCGTATG650.001574617217.07692330
Produced by FastQC (version 0.11.5)