FastQCFastQC Report
Fri 10 Feb 2017
ERR1632307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531878
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6040.11355987651303495No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5680.10679140705199314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2650.023.7358471
GCTAGAT705.0934195E-621.1428593
TAGACCC450.003823145820.5555554
AGTGCGT450.003823145820.5555558
TTACACT450.003823145820.5555554
GTGCTAG1103.8400685E-818.51
TGCTAGA801.6140108E-518.52
TGTACTG801.6140108E-518.55
CCAAATT609.2279224E-418.513
GTATCAA3450.017.6956522
CTCTATG1156.385744E-817.695651
AGATAGG650.001578425817.07692335
TTAGAAC650.001578425817.0769233
GCGGGTA650.001578425817.07692323
TCTATGG1301.3880708E-817.0769232
CTAGATT904.4387336E-516.4444454
ATCTCGC1851.8189894E-1116.011
TACCCTA700.002590037915.8571435
AGGCGAT700.002590037915.85714326
TAACCCG700.002590037915.85714328
Produced by FastQC (version 0.11.5)