##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632307.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	531878
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.904604063337832	31.0	31.0	34.0	30.0	34.0
2	32.09302321208999	33.0	31.0	34.0	30.0	34.0
3	32.115088422532985	34.0	31.0	34.0	30.0	34.0
4	35.8556041047007	37.0	35.0	37.0	35.0	37.0
5	35.414074280192075	37.0	35.0	37.0	33.0	37.0
6	35.3988038610358	37.0	35.0	37.0	33.0	37.0
7	35.39767578279229	37.0	35.0	37.0	33.0	37.0
8	35.352421043923606	37.0	35.0	37.0	33.0	37.0
9	37.02384569393733	39.0	37.0	39.0	33.0	39.0
10	36.82988956113996	38.0	37.0	39.0	33.0	39.0
11	36.981065206682736	39.0	37.0	39.0	33.0	39.0
12	36.92210807741625	39.0	37.0	39.0	33.0	39.0
13	36.96941967894893	39.0	37.0	39.0	33.0	39.0
14	38.12303573375849	40.0	38.0	41.0	33.0	41.0
15	38.099479579903665	40.0	38.0	41.0	33.0	41.0
16	37.99596712027946	40.0	37.0	41.0	33.0	41.0
17	38.02010611456011	40.0	37.0	41.0	33.0	41.0
18	38.02173806775238	40.0	37.0	41.0	33.0	41.0
19	38.08513794516788	40.0	38.0	41.0	34.0	41.0
20	38.05781024971892	40.0	37.0	41.0	34.0	41.0
21	38.017242675952005	40.0	37.0	41.0	33.0	41.0
22	37.99681881935331	40.0	37.0	41.0	33.0	41.0
23	37.952088636867856	39.0	37.0	40.0	33.0	41.0
24	37.87145172389157	39.0	37.0	41.0	33.0	41.0
25	37.79840489736368	39.0	37.0	40.0	33.0	41.0
26	37.66014198744825	39.0	37.0	40.0	33.0	41.0
27	37.57313707278737	39.0	37.0	40.0	33.0	41.0
28	37.492069609948146	39.0	37.0	40.0	32.0	41.0
29	37.344379726177806	39.0	37.0	40.0	32.0	41.0
30	37.274700213206785	39.0	36.0	40.0	32.0	41.0
31	37.1797141449731	39.0	36.0	40.0	31.0	41.0
32	37.08703499674738	39.0	36.0	40.0	31.0	41.0
33	36.95089099379933	39.0	36.0	40.0	31.0	41.0
34	36.93458274265903	39.0	36.0	40.0	31.0	41.0
35	36.89362786202851	39.0	36.0	40.0	31.0	41.0
36	36.86133098191691	39.0	36.0	40.0	31.0	41.0
37	36.839619235990206	39.0	36.0	40.0	31.0	41.0
38	36.750751112097134	39.0	35.0	40.0	31.0	41.0
39	36.586115612978915	38.0	35.0	40.0	30.0	41.0
40	36.58799198312395	38.0	35.0	40.0	30.0	41.0
41	36.58790737725569	38.0	35.0	40.0	30.0	41.0
42	36.49125739361282	38.0	35.0	40.0	30.0	41.0
43	35.56604898115733	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	1.0
15	4.0
16	7.0
17	12.0
18	37.0
19	71.0
20	100.0
21	201.0
22	354.0
23	599.0
24	937.0
25	1408.0
26	2032.0
27	2990.0
28	4181.0
29	6000.0
30	8009.0
31	10817.0
32	14581.0
33	20444.0
34	28730.0
35	41755.0
36	65688.0
37	103694.0
38	145289.0
39	73934.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.15308773816552	18.893618461376484	12.704981217497247	26.248312582960754
2	19.834059690380123	20.988647772609507	33.430598746329046	25.746693790681324
3	21.016473702616015	22.014070895957342	29.348271596117907	27.621183805308736
4	16.474078642094618	15.79572759166576	32.5678069030868	35.162386863152825
5	16.13809933857012	35.0535273126544	33.00587728764867	15.8024960611268
6	35.6841230507748	33.34035248684849	15.215331335381421	15.76019312699529
7	29.89350941381294	29.14972982526068	20.48044852390962	20.476312237016757
8	26.921963307375	32.79267049962586	19.970557157844468	20.31480903515468
9	26.62546674237325	14.313056753616433	19.21192453908603	39.84955196492429
10	17.535224243153504	26.50288223991216	32.73288235271998	23.22901116421435
11	36.18686992129774	21.258634498888842	20.83015277939678	21.724342800416636
12	22.112777742264203	24.954218824617673	27.817657432719535	25.115346000398585
13	30.98473710136535	19.312511515798736	23.864495241389942	25.83825614144597
14	23.798126638063614	20.650976351719756	23.462147334539125	32.088749675677505
15	26.619450325074546	26.82231639586522	21.237765051383963	25.320468227676272
16	26.13193251083895	25.528974689684475	22.84508853534081	25.49400426413576
17	24.73856786706726	25.886387479835598	24.26928731776836	25.105757335328775
18	24.237137087828412	24.764325653627335	25.53160687225266	25.46693038629159
19	25.703826817428055	24.748344545177652	25.323852462406794	24.223976174987495
20	25.79764532467972	24.199910505792683	25.312571679971725	24.689872489555874
21	25.639150331466993	24.846487352362757	24.12357721131538	25.390785104854874
22	25.86326187584371	24.474785571127214	24.11210841583972	25.549844137189353
23	25.38740087012435	24.878449569262123	24.19859441450859	25.535555146104933
24	26.205257596666904	24.74740447997473	24.1136125201644	24.933725403193964
25	25.974753608910312	24.10778411590628	24.44996784977006	25.467494425413346
26	25.68182929167967	24.63572473386754	24.188629723357614	25.493816251095176
27	25.636706161939394	24.35878152508658	24.144258645779672	25.860253667194357
28	25.07511120971351	24.519156648705152	24.78839132282215	25.61734081875919
29	25.022843584431016	24.911539864404993	24.739695945310768	25.32592060585322
30	24.99614573266802	25.629561666397183	24.365361981507036	25.008930619427765
31	25.72958460398813	24.615231312443832	24.612599129875647	25.042584953692387
32	24.699837180706854	24.638168903395137	24.745524349568885	25.91646956632912
33	24.99501765442451	24.24371754424887	24.82148161796502	25.939783183361598
34	25.483663546903614	24.05927675143548	24.811516926814043	25.645542774846863
35	25.25861193732397	24.1136125201644	25.199199816499274	25.428575726012355
36	25.026791858283293	24.30519780852	25.089024174716755	25.578986158479957
37	25.555860554488056	23.034041641128226	25.335697283963615	26.0744005204201
38	24.57048420878472	23.701111909122016	26.532400287283924	25.196003594809333
39	24.364045890222947	23.320573514979	26.851458417155815	25.46392217764224
40	24.105903985500436	22.834371792027493	27.516836567784342	25.54288765468773
41	23.507082451238816	23.154746013183473	27.708233843099357	25.62993769247835
42	23.874647945581508	22.808049966345667	27.44275942979405	25.874542658278777
43	23.248940546516305	22.787556544921955	27.833262515088048	26.13024039347369
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	14.5
2	17.0
3	38.5
4	60.0
5	60.0
6	82.0
7	104.0
8	100.5
9	97.0
10	170.0
11	243.0
12	243.0
13	400.5
14	558.0
15	902.5
16	1247.0
17	1259.5
18	1272.0
19	1272.0
20	1722.0
21	2172.0
22	2612.0
23	3052.0
24	3748.0
25	4444.0
26	4444.0
27	5130.5
28	5817.0
29	7909.5
30	10002.0
31	11301.5
32	12601.0
33	12601.0
34	14307.5
35	16014.0
36	18028.0
37	20042.0
38	23065.5
39	26089.0
40	26089.0
41	28659.5
42	31230.0
43	30769.0
44	30308.0
45	32695.5
46	35083.0
47	35083.0
48	37017.0
49	38951.0
50	41386.0
51	43821.0
52	46169.5
53	48518.0
54	48518.0
55	44133.0
56	39748.0
57	37470.5
58	35193.0
59	32383.5
60	29574.0
61	29574.0
62	27690.0
63	25806.0
64	23172.5
65	20539.0
66	17960.5
67	15382.0
68	15382.0
69	13334.5
70	11287.0
71	9918.5
72	8550.0
73	7033.5
74	5517.0
75	5517.0
76	4455.5
77	3394.0
78	2866.5
79	2339.0
80	1843.5
81	1348.0
82	1348.0
83	1019.5
84	691.0
85	570.0
86	449.0
87	310.0
88	171.0
89	171.0
90	134.0
91	97.0
92	62.5
93	28.0
94	17.0
95	6.0
96	6.0
97	5.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	531878.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.85146751753896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.25301311092814	61.23799387456159
2	5.775067378796154	7.836935933279725
3	1.4480795699689168	2.9476297171367327
4	0.6507043454180624	1.766049790266205
5	0.3781258277203812	1.2828196158555991
6	0.22546197794880812	0.9178755643940186
7	0.1792648157274369	0.8514366574957456
8	0.10954019992405072	0.5945970653609162
9	0.10079834375175058	0.6155383992204269
>10	0.7138958689217434	9.664706904671792
>50	0.10531268058914083	4.951649878214615
>100	0.06017867039438571	7.111214589883887
>500	5.57209911059117E-4	0.22155200965877572
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	604	0.11355987651303495	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	568	0.10679140705199314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.8801304058449494E-4	0.0	0.0	0.0	0.0
3	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
4	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
5	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
6	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
7	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
8	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
9	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
10	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
11	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
12	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
13	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
14	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
15	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
16	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
17	1.8801304058449494E-4	1.8801304058449494E-4	0.0	0.0	0.0
18	1.8801304058449494E-4	1.8801304058449494E-4	0.0	1.8801304058449494E-4	0.0
19	1.8801304058449494E-4	1.8801304058449494E-4	0.0	1.8801304058449494E-4	0.0
20	1.8801304058449494E-4	1.8801304058449494E-4	0.0	3.760260811689899E-4	0.0
21	1.8801304058449494E-4	1.8801304058449494E-4	0.0	3.760260811689899E-4	0.0
22	1.8801304058449494E-4	1.8801304058449494E-4	0.0	5.640391217534848E-4	0.0
23	1.8801304058449494E-4	3.760260811689899E-4	0.0	0.0011280782435069696	0.0
24	1.8801304058449494E-4	3.760260811689899E-4	0.0	0.0020681434464294443	0.0
25	1.8801304058449494E-4	3.760260811689899E-4	0.0	0.0026321825681829294	0.0
26	1.8801304058449494E-4	3.760260811689899E-4	0.0	0.004512312974027878	0.0
27	1.8801304058449494E-4	3.760260811689899E-4	0.0	0.01410097804383712	0.0
28	1.8801304058449494E-4	3.760260811689899E-4	0.0	0.0483193514302152	0.0
29	1.8801304058449494E-4	3.760260811689899E-4	0.0	0.09381850725166298	0.0
30	1.8801304058449494E-4	3.760260811689899E-4	0.0	0.16112717578091215	0.0
31	1.8801304058449494E-4	3.760260811689899E-4	0.0	0.3685055595456101	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	265	0.0	23.735847	1
GCTAGAT	70	5.0934195E-6	21.142859	3
TAGACCC	45	0.0038231458	20.555555	4
AGTGCGT	45	0.0038231458	20.555555	8
TTACACT	45	0.0038231458	20.555555	4
GTGCTAG	110	3.8400685E-8	18.5	1
TGCTAGA	80	1.6140108E-5	18.5	2
TGTACTG	80	1.6140108E-5	18.5	5
CCAAATT	60	9.2279224E-4	18.5	13
GTATCAA	345	0.0	17.695652	2
CTCTATG	115	6.385744E-8	17.69565	1
AGATAGG	65	0.0015784258	17.076923	35
TTAGAAC	65	0.0015784258	17.076923	3
GCGGGTA	65	0.0015784258	17.076923	23
TCTATGG	130	1.3880708E-8	17.076923	2
CTAGATT	90	4.4387336E-5	16.444445	4
ATCTCGC	185	1.8189894E-11	16.0	11
TACCCTA	70	0.0025900379	15.857143	5
AGGCGAT	70	0.0025900379	15.857143	26
TAACCCG	70	0.0025900379	15.857143	28
>>END_MODULE