FastQCFastQC Report
Fri 10 Feb 2017
ERR1632306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270538
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5150.024.4271851
GACGCTA609.2375476E-418.529
TATACTG752.0674826E-417.2666665
CCGATAA904.446548E-516.4444459
GTTCTAA1059.344569E-615.8571421
TCTAATA1059.344569E-615.8571422
TATAACT700.002592695215.8571424
GTATCAA7800.015.6538472
ATACCTT957.06061E-515.5789486
GTATAGA855.364705E-415.2352931
TTATACT1152.2100963E-514.4782614
TATATAT1304.4445587E-614.2307692
TTAACAC1051.6558476E-414.0952383
TCGACCG1051.6558476E-414.09523833
CTTATAC7550.013.966886537
TCTTATA11450.013.89519737
TAATACT1601.7834827E-713.8754
TCGATGC1601.7834827E-713.87529
AATACGA1203.3008975E-513.8749995
CTAATAC2405.456968E-1213.8749993
Produced by FastQC (version 0.11.5)