FastQCFastQC Report
Fri 10 Feb 2017
ERR1632305.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632305.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2947327
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.023.1652181
TCTTATA29650.015.84822937
CTTATAC17500.015.11714337
GTATCAA19150.013.9112262
TTTAGAC3000.013.5666673
CTCTTAT46750.013.37540137
CGATACG1000.001833662512.95000126
ATAATAC3150.012.9206343
TGTACCG2602.3646862E-1112.8076935
AGACCGT2552.237357E-1012.3333336
CGTTATT1951.4697434E-712.3333322
TCTATAC2307.921699E-912.0652163
ATAAGAC1851.040813E-612.03
TGTCGCG1257.224585E-411.84000110
ATTCGTA2351.08957465E-811.80851124
TTACACA4700.011.8085114
ATAGTAC3300.011.7727273
AATCGTA1100.003747430611.77272718
ATCAACG28050.011.6737972
ACACCGT2705.802576E-1011.6481496