##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632305.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2947327
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.952093880319353	33.0	31.0	34.0	30.0	34.0
2	32.13433392358568	33.0	31.0	34.0	30.0	34.0
3	32.162541516431666	34.0	31.0	34.0	30.0	34.0
4	35.882936640556004	37.0	35.0	37.0	35.0	37.0
5	35.496010113570705	37.0	35.0	37.0	33.0	37.0
6	35.47202872297509	37.0	35.0	37.0	33.0	37.0
7	35.467879539664246	37.0	35.0	37.0	33.0	37.0
8	35.430611533772804	37.0	35.0	37.0	33.0	37.0
9	37.10877585011775	39.0	37.0	39.0	33.0	39.0
10	36.905622959379805	39.0	37.0	39.0	33.0	39.0
11	37.0594949254019	39.0	37.0	39.0	33.0	39.0
12	36.990039110013925	39.0	37.0	39.0	33.0	39.0
13	37.056354452695615	39.0	37.0	39.0	33.0	39.0
14	38.22616017835822	40.0	38.0	41.0	34.0	41.0
15	38.19804928329975	40.0	38.0	41.0	34.0	41.0
16	38.082887986300804	40.0	38.0	41.0	33.0	41.0
17	38.11622090117588	40.0	38.0	41.0	33.0	41.0
18	38.13012875734521	40.0	38.0	41.0	33.0	41.0
19	38.18313950233551	40.0	38.0	41.0	34.0	41.0
20	38.14621825131721	40.0	38.0	41.0	34.0	41.0
21	38.115261048400804	40.0	38.0	41.0	34.0	41.0
22	38.08769335740486	40.0	38.0	41.0	34.0	41.0
23	38.03381742168412	40.0	37.0	41.0	33.0	41.0
24	37.96758452658969	40.0	37.0	41.0	33.0	41.0
25	37.87746897443005	39.0	37.0	41.0	33.0	41.0
26	37.75056381595934	39.0	37.0	40.0	33.0	41.0
27	37.66157810110653	39.0	37.0	40.0	33.0	41.0
28	37.57447951991754	39.0	37.0	40.0	33.0	41.0
29	37.417791442890454	39.0	37.0	40.0	32.0	41.0
30	37.35298730001795	39.0	37.0	40.0	32.0	41.0
31	37.248756924494636	39.0	36.0	40.0	32.0	41.0
32	37.15657203968206	39.0	36.0	40.0	31.0	41.0
33	36.99843688874699	39.0	36.0	40.0	31.0	41.0
34	36.98738450127862	39.0	36.0	40.0	31.0	41.0
35	36.955071493594026	39.0	36.0	40.0	31.0	41.0
36	36.93200381226786	39.0	36.0	40.0	31.0	41.0
37	36.89879405983795	39.0	36.0	40.0	31.0	41.0
38	36.828496125472334	39.0	36.0	40.0	31.0	41.0
39	36.63964466786346	39.0	35.0	40.0	30.0	41.0
40	36.63558573582097	39.0	35.0	40.0	30.0	41.0
41	36.62768874984011	39.0	35.0	40.0	30.0	41.0
42	36.50816282007392	38.0	35.0	40.0	30.0	41.0
43	35.594335138245604	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	1.0
12	2.0
13	1.0
14	5.0
15	11.0
16	27.0
17	78.0
18	122.0
19	313.0
20	550.0
21	1033.0
22	1894.0
23	3184.0
24	4979.0
25	7600.0
26	11066.0
27	16198.0
28	23191.0
29	31936.0
30	43670.0
31	58501.0
32	78601.0
33	107281.0
34	152871.0
35	222905.0
36	347839.0
37	563663.0
38	830884.0
39	438916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73404342307454	18.36050088775355	12.062455234861961	26.843000454309955
2	20.06360339385484	20.07483390882654	32.87344770363112	26.9881149936875
3	20.870809380838978	21.566592373360677	28.596792958501045	28.965805287299307
4	15.921816615529934	15.489865902222592	32.75442460236004	35.833892879887436
5	16.628524761589063	34.3002998988575	32.79035546445983	16.280819875093602
6	36.452181926199565	33.26129065420973	15.011873470436093	15.274653949154606
7	30.983905077380285	28.63733817116323	20.40391174783117	19.97484500362532
8	28.142177640960774	32.040998504746845	19.211136056501367	20.605687797791013
9	27.252659782915163	13.693492442474147	18.761677954295536	40.29216982031515
10	17.820723659098565	25.603843754018474	32.31826668706933	24.257165899813625
11	37.75651632818482	20.460607187461726	20.41700836045678	21.365868123896668
12	22.945095674826714	23.9540777117707	28.10332888071124	24.99749773269135
13	31.924655798287738	18.630779686135945	22.789259556201262	26.655304959375055
14	23.871867627853984	19.326019813885598	23.61390507398738	33.188207484273036
15	26.626838487890893	26.126181451871478	20.81445323169095	26.43252682854668
16	26.536587219538244	24.61413341648212	22.75393941697002	26.09533994700961
17	25.29536084730334	24.87813534093774	24.07069185061583	25.7558119611431
18	25.49584759342957	24.054100546020173	24.70692936345373	25.743122497096522
19	26.052317913824968	24.127149786908614	24.673203889490374	25.147328409776044
20	26.242320584041064	24.037441383328012	24.307584465517397	25.412653567113523
21	26.548224883088984	23.903523429873914	23.8979929950087	25.650258692028405
22	26.76160466755131	23.844622602106927	23.746364078366604	25.647408651975162
23	26.282899725751502	23.84431724067265	23.90674668945794	25.96603634411791
24	26.170933866517014	24.004156986991944	23.620215876962412	26.20469326952863
25	26.34268270877307	23.5457076869991	24.04439683821985	26.06721276600798
26	26.038949868813333	23.90192876460603	24.04158072721486	26.017540639365773
27	25.953720099602112	23.838074295794122	24.04840046591369	26.159805138690075
28	25.94052169983175	23.802889872755888	24.231176248851927	26.025412178560437
29	25.822007534284452	24.107470938921946	24.378631892558918	25.691889634234684
30	25.666646422334544	24.265851736166365	24.090574272892013	25.976927568607078
31	26.069553870337426	24.01911969727146	23.98386741613672	25.92745901625439
32	25.454454154561063	24.089997479071716	24.189477448549145	26.26607091781808
33	25.35772243799212	23.81527397536819	24.68436654636557	26.14263704027412
34	25.98825987072354	23.247064204277297	24.486628053147818	26.27804787185134
35	25.570491499585895	23.522466288945882	25.039332249187147	25.867709962281076
36	25.554985924534336	23.394350202743027	24.718261665570193	26.332402207152445
37	25.309577118521293	22.832247660337654	25.42133940346626	26.436835817674798
38	24.86398692781629	22.91445774425437	25.97784365290991	26.243711675019433
39	24.689489832651756	22.57265651215491	26.540081911508288	26.19777174368504
40	24.506781907810026	22.37373050224831	27.039212140356327	26.08027544958534
41	23.89751798833316	22.37800556232817	27.452467948076343	26.272008501262327
42	23.752742739438144	22.486442800544356	27.508891955320873	26.25192250469663
43	23.323608137135786	22.06653689936678	27.837291213360444	26.77256375013699
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	77.0
1	102.5
2	128.0
3	314.5
4	501.0
5	501.0
6	639.5
7	778.0
8	782.0
9	786.0
10	1104.0
11	1422.0
12	1422.0
13	2479.0
14	3536.0
15	5100.5
16	6665.0
17	6895.0
18	7125.0
19	7125.0
20	8877.0
21	10629.0
22	11427.0
23	12225.0
24	15377.5
25	18530.0
26	18530.0
27	22950.5
28	27371.0
29	34229.0
30	41087.0
31	49113.5
32	57140.0
33	57140.0
34	68423.5
35	79707.0
36	91652.5
37	103598.0
38	116080.0
39	128562.0
40	128562.0
41	142491.0
42	156420.0
43	169017.5
44	181615.0
45	191107.5
46	200600.0
47	200600.0
48	207792.5
49	214985.0
50	221037.0
51	227089.0
52	229293.5
53	231498.0
54	231498.0
55	226469.0
56	221440.0
57	214613.5
58	207787.0
59	200794.5
60	193802.0
61	193802.0
62	179602.0
63	165402.0
64	148686.5
65	131971.0
66	116534.0
67	101097.0
68	101097.0
69	87188.0
70	73279.0
71	62336.5
72	51394.0
73	42400.0
74	33406.0
75	33406.0
76	27557.0
77	21708.0
78	17907.5
79	14107.0
80	11795.5
81	9484.0
82	9484.0
83	7370.5
84	5257.0
85	4056.0
86	2855.0
87	2133.0
88	1411.0
89	1411.0
90	1027.5
91	644.0
92	403.5
93	163.0
94	95.0
95	27.0
96	27.0
97	22.0
98	17.0
99	9.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2947327.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.29780873461605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.05392905298844	53.204295245890485
2	9.11978659523295	11.545250152111409
3	2.667349888592245	5.06512209229234
4	1.2493555000674725	3.163258619392458
5	0.6913903577221692	2.1881747312027815
6	0.43199602456468766	1.6406641042206054
7	0.3068497561340112	1.3596042021823953
8	0.22254635041025844	1.1269357058284302
9	0.1738067017269134	0.9901425026434161
>10	0.9506221073813332	11.110773475651397
>50	0.08917494628407245	3.915903726485804
>100	0.04281669910234591	4.336869215157068
>500	1.6115133991556987E-4	0.059367065342306534
>1k	2.148684531563682E-4	0.2936391615989734
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.785809650574911E-5
7	0.0	0.0	0.0	0.0	6.785809650574911E-5
8	0.0	0.0	0.0	3.3929048252874556E-5	6.785809650574911E-5
9	0.0	0.0	0.0	3.3929048252874556E-5	6.785809650574911E-5
10	0.0	0.0	0.0	3.3929048252874556E-5	6.785809650574911E-5
11	0.0	0.0	0.0	6.785809650574911E-5	6.785809650574911E-5
12	0.0	0.0	0.0	6.785809650574911E-5	6.785809650574911E-5
13	0.0	0.0	0.0	1.0178714475862366E-4	6.785809650574911E-5
14	0.0	0.0	0.0	1.6964524126437277E-4	6.785809650574911E-5
15	0.0	0.0	0.0	1.6964524126437277E-4	6.785809650574911E-5
16	0.0	0.0	0.0	2.3750333777012187E-4	6.785809650574911E-5
17	0.0	0.0	0.0	4.0714857903449464E-4	6.785809650574911E-5
18	0.0	0.0	0.0	4.0714857903449464E-4	6.785809650574911E-5
19	0.0	3.3929048252874556E-5	0.0	5.428647720459929E-4	6.785809650574911E-5
20	0.0	3.3929048252874556E-5	0.0	7.125100133103656E-4	1.0178714475862366E-4
21	0.0	3.3929048252874556E-5	0.0	8.821552545747384E-4	1.0178714475862366E-4
22	0.0	3.3929048252874556E-5	0.0	0.0013910909783678568	1.0178714475862366E-4
23	0.0	3.3929048252874556E-5	0.0	0.0018660976539081006	1.0178714475862366E-4
24	0.0	3.3929048252874556E-5	0.0	0.003664337211310452	1.0178714475862366E-4
25	0.0	3.3929048252874556E-5	0.0	0.004885782948413936	1.0178714475862366E-4
26	0.0	3.3929048252874556E-5	0.0	0.007905468242919772	1.0178714475862366E-4
27	0.0	3.3929048252874556E-5	0.0	0.024157482356046683	1.0178714475862366E-4
28	0.0	3.3929048252874556E-5	0.0	0.07457604805981827	1.0178714475862366E-4
29	0.0	3.3929048252874556E-5	0.0	0.142807364096349	1.0178714475862366E-4
30	0.0	3.3929048252874556E-5	0.0	0.2360104596469954	1.0178714475862366E-4
31	0.0	3.3929048252874556E-5	0.0	0.5251538088579923	1.0178714475862366E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1150	0.0	23.165218	1
TCTTATA	2965	0.0	15.848229	37
CTTATAC	1750	0.0	15.117143	37
GTATCAA	1915	0.0	13.911226	2
TTTAGAC	300	0.0	13.566667	3
CTCTTAT	4675	0.0	13.375401	37
CGATACG	100	0.0018336625	12.950001	26
ATAATAC	315	0.0	12.920634	3
TGTACCG	260	2.3646862E-11	12.807693	5
AGACCGT	255	2.237357E-10	12.333333	6
CGTTATT	195	1.4697434E-7	12.333332	2
TCTATAC	230	7.921699E-9	12.065216	3
ATAAGAC	185	1.040813E-6	12.0	3
TGTCGCG	125	7.224585E-4	11.840001	10
ATTCGTA	235	1.08957465E-8	11.808511	24
TTACACA	470	0.0	11.808511	4
ATAGTAC	330	0.0	11.772727	3
AATCGTA	110	0.0037474306	11.772727	18
ATCAACG	2805	0.0	11.673797	2
ACACCGT	270	5.802576E-10	11.648149	6
>>END_MODULE