FastQCFastQC Report
Fri 10 Feb 2017
ERR1632304.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632304.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104525
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1360.130112413298254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1200.11480507055728294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCT200.001834290737.09
ACGCTGA200.001834290737.011
AATAGAC200.001834290737.018
TAGACAC250.005473789329.620
GATATAC250.005473789329.63
CAAATCT250.005473789329.627
CCAGTCA250.005473789329.619
GAAATAG400.001919526523.12516
ACCATTG450.003802927220.5555558
GGTATCA1001.256376E-820.351
AAGCCGC500.006993211318.516
ATATACA500.006993211318.54
AACGCAG2050.016.2439045
ATCAACG2200.015.9772732
CTCTTAT2500.015.5437
CAACGCA2150.015.4883744
CTTATAC1101.4268486E-515.13636437
GTATCAA1353.8723738E-715.0740752
ATGGGAA750.00407130114.817
TATCAAC2400.014.6458331
Produced by FastQC (version 0.11.5)