FastQCFastQC Report
Fri 10 Feb 2017
ERR1632303.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632303.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208844
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAC303.589486E-430.8333343
CGAAAGC250.005486174529.619
TCCGTAG250.005486174529.623
GGTATCA1250.026.641
GTATTAA358.845007E-426.428571
GAATATG358.845007E-426.428571
TTCCCAA451.3181525E-424.66666627
CGGGTTA400.001925927623.1259
TAAATCC400.001925927623.1254
AGAGCGA400.001925927623.12515
AAAAGTT502.6918532E-422.222
TTCAACG502.6918532E-422.24
AAGTTCC502.6918532E-422.224
GTATCAA1600.020.81252
GTATTGC450.003815488220.5555556
GTTTAGA450.003815488220.5555552
TTTAAAT450.003815488220.5555552
GGATCCA450.003815488220.55555519
GGTTTAG450.003815488220.5555551
TATAGGG450.003815488220.5555552
Produced by FastQC (version 0.11.5)