FastQCFastQC Report
Fri 10 Feb 2017
ERR1632302.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632302.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408183
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAATG250.00549224229.615
AACATAA250.00549224229.631
TTATTCT405.930889E-527.7500024
CGGCTAT358.8595593E-426.4285728
GTAGACT802.7148417E-823.12500216
GTACTAT400.001929065323.1250021
CTTATAC3150.022.9047637
TTTATTC652.6758116E-622.769233
TATACAC903.810783E-922.6111137
GGTATCA1650.022.4242421
TTGTAGA855.160109E-821.7647062
CTAGACA1351.8189894E-1220.5555554
AGGACTA450.003821645220.5555555
TAAAACA450.003821645220.5555554
GGCCCTT450.003821645220.55555533
ACATGTA1001.2836608E-820.358
TCTAGAC1403.6379788E-1219.8214283
GCGTTAT851.2412856E-619.5882341
TGACCCG851.2412856E-619.58823413
TAGACTC951.6695049E-719.47368417
Produced by FastQC (version 0.11.5)