##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632302.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	408183
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.206941494378746	33.0	31.0	34.0	30.0	34.0
2	32.401280798073415	34.0	31.0	34.0	31.0	34.0
3	32.483861405301056	34.0	31.0	34.0	31.0	34.0
4	36.05615128508536	37.0	35.0	37.0	35.0	37.0
5	35.77141135226112	37.0	35.0	37.0	35.0	37.0
6	35.769150111592104	37.0	35.0	37.0	35.0	37.0
7	35.74482523769976	37.0	35.0	37.0	35.0	37.0
8	35.725287432352644	37.0	35.0	37.0	33.0	37.0
9	37.45695925601997	39.0	37.0	39.0	35.0	39.0
10	37.33376696236737	39.0	37.0	39.0	34.0	39.0
11	37.434689832746585	39.0	37.0	39.0	35.0	39.0
12	37.37323700399086	39.0	37.0	39.0	35.0	39.0
13	37.40852509781152	39.0	37.0	39.0	35.0	39.0
14	38.64707741380704	40.0	38.0	41.0	35.0	41.0
15	38.61905566865842	40.0	38.0	41.0	35.0	41.0
16	38.62390153436081	40.0	38.0	41.0	35.0	41.0
17	38.59639181445577	40.0	38.0	41.0	35.0	41.0
18	38.5935181033997	40.0	38.0	41.0	35.0	41.0
19	38.61385946989463	40.0	38.0	41.0	35.0	41.0
20	38.57854197749539	40.0	38.0	41.0	34.0	41.0
21	38.52343434194957	40.0	38.0	41.0	34.0	41.0
22	38.488905711408854	40.0	38.0	41.0	34.0	41.0
23	38.4361230134523	40.0	38.0	41.0	34.0	41.0
24	38.37751204729251	40.0	38.0	41.0	34.0	41.0
25	38.2987581550432	40.0	38.0	41.0	34.0	41.0
26	38.197673592481806	40.0	38.0	41.0	34.0	41.0
27	38.08708348951328	40.0	38.0	41.0	34.0	41.0
28	38.00710465648986	40.0	38.0	41.0	33.0	41.0
29	37.93828503391861	40.0	37.0	41.0	33.0	41.0
30	37.85496456246341	40.0	37.0	41.0	33.0	41.0
31	37.77362359529917	40.0	37.0	41.0	33.0	41.0
32	37.666029697464126	40.0	37.0	41.0	33.0	41.0
33	37.580815467572144	39.0	37.0	41.0	33.0	41.0
34	37.49423910353934	39.0	37.0	41.0	32.0	41.0
35	37.47044340406141	39.0	37.0	41.0	32.0	41.0
36	37.42573551568782	39.0	37.0	40.0	32.0	41.0
37	37.37204146179532	39.0	36.0	40.0	32.0	41.0
38	37.30488775867687	39.0	36.0	40.0	32.0	41.0
39	37.25022355169128	39.0	36.0	40.0	32.0	41.0
40	37.18412084775701	39.0	36.0	40.0	31.0	41.0
41	37.1315586391398	39.0	36.0	40.0	31.0	41.0
42	37.070661933495515	39.0	36.0	40.0	31.0	41.0
43	36.10248589480699	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	8.0
18	9.0
19	32.0
20	52.0
21	79.0
22	158.0
23	278.0
24	481.0
25	735.0
26	1191.0
27	1678.0
28	2430.0
29	3518.0
30	4821.0
31	6614.0
32	8739.0
33	11889.0
34	16824.0
35	24708.0
36	39671.0
37	69053.0
38	127153.0
39	88060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.476822895613	17.38901424116144	11.24985606945904	27.884306793766523
2	20.909249037809023	19.79430794521085	31.07821736819025	28.21822564878988
3	21.32695384178175	19.905287579345536	28.028114840647454	30.739643738225258
4	16.081757447027435	14.50256380104022	31.10443110075628	38.31124765117607
5	17.481374775529606	33.80885534184422	31.760754367526328	16.949015515099845
6	38.71792798818177	31.82959603903151	13.990293569305923	15.462182403480792
7	32.658880943106396	27.82722455369283	18.838854141402265	20.67504036179851
8	29.44169649397452	30.601470418905247	18.58235154330288	21.374481543817357
9	27.944818868007733	12.838114277174698	17.559770985072873	41.6572958697447
10	18.30257507049534	25.132844826952617	30.654632848501773	25.909947254050262
11	40.01710017320663	19.264643554484138	18.77295232775495	21.945303944554283
12	24.696765911368185	22.977194052667553	26.07800912825865	26.248030907705612
13	33.19418006139403	17.213112745998732	22.033009703980813	27.559697488626423
14	25.200461557683685	18.135003172596605	21.56900213874659	35.095533130973116
15	27.786311531837438	25.294292020980784	19.52359603413175	27.395800413050033
16	28.221165506647754	22.98772854332493	20.95775669246392	27.833349257563395
17	26.898229470605095	23.761891112564708	22.06436818779812	27.27551122903208
18	27.311279499636193	22.07784253631337	22.92476658753549	27.686111376514944
19	27.521234348319258	22.616081512458873	22.24614939867657	27.616534740545294
20	27.723349576047994	22.53278553981915	22.70623715343363	27.03762773069922
21	28.737355548859213	22.383587753532115	22.295147029641115	26.583909667967554
22	28.84735523037461	22.27187315493296	21.40559503947984	27.47517657521259
23	27.500900331468976	22.37672808519708	22.119735510788054	28.002636072545894
24	27.42985376657039	22.376238108887435	21.89287647942222	28.301031645119956
25	27.966377825632133	22.153543876153588	22.106016174117983	27.7740621240963
26	27.92546480377674	22.187842217828766	22.016105521298044	27.870587457096452
27	27.738048865337362	22.199111672950615	22.38750756400928	27.675331897702748
28	27.041057564866737	22.30984631893048	22.687373065512283	27.9617230506905
29	26.909743913881762	22.530825634580566	22.642295245024904	27.917135206512768
30	26.30192830176661	22.964699656771597	23.155300441223666	27.57807160023813
31	27.842658807446657	22.719221525639234	21.81644017511753	27.62167949179657
32	26.857561436904525	22.443364863308858	22.4147012491946	28.284372450592016
33	26.684844787754514	22.03080481058741	23.01933201529706	28.265018386361017
34	27.372036562032225	21.53788864308411	23.166814884500333	27.923259910383337
35	26.74707177907948	21.902186029305483	23.836612499785637	27.514129691829396
36	26.548386385518256	21.779446963739304	24.077680844131187	27.594485806611253
37	27.02219347694539	21.194905226332306	24.035297893346858	27.747603403375447
38	25.994223179309277	20.855106655593204	25.429525482442923	27.721144682654593
39	26.03832104717737	20.80414911939008	25.75854457436983	27.398985259062727
40	25.38836747243271	21.48668611872616	25.750704953415504	27.37424145542563
41	25.23965966245532	20.73849229389759	26.93669261091226	27.085155432734826
42	24.999081294419415	20.61281337047354	27.066046356658656	27.322058978448393
43	23.46766033862263	20.171099727328183	27.55308280844621	28.80815712560298
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	9.0
4	17.0
5	17.0
6	19.5
7	22.0
8	25.0
9	28.0
10	31.5
11	35.0
12	35.0
13	68.5
14	102.0
15	155.5
16	209.0
17	217.5
18	226.0
19	226.0
20	290.5
21	355.0
22	407.5
23	460.0
24	606.0
25	752.0
26	752.0
27	985.5
28	1219.0
29	1630.5
30	2042.0
31	2696.5
32	3351.0
33	3351.0
34	4558.0
35	5765.0
36	7367.5
37	8970.0
38	10368.0
39	11766.0
40	11766.0
41	13590.0
42	15414.0
43	17678.5
44	19943.0
45	22888.0
46	25833.0
47	25833.0
48	27667.5
49	29502.0
50	31899.5
51	34297.0
52	35800.5
53	37304.0
54	37304.0
55	37123.0
56	36942.0
57	36197.5
58	35453.0
59	35299.5
60	35146.0
61	35146.0
62	33225.5
63	31305.0
64	27537.0
65	23769.0
66	21244.0
67	18719.0
68	18719.0
69	15201.0
70	11683.0
71	9754.0
72	7825.0
73	5936.5
74	4048.0
75	4048.0
76	3146.5
77	2245.0
78	1847.0
79	1449.0
80	1209.0
81	969.0
82	969.0
83	724.5
84	480.0
85	378.0
86	276.0
87	209.0
88	142.0
89	142.0
90	120.5
91	99.0
92	56.5
93	14.0
94	8.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	408183.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.64592437952596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.16081159333541	49.66819629587996
2	6.697857227190402	7.722083424469586
3	2.2174277765740613	3.8347702177634613
4	1.1280806442286506	2.601170060448468
5	0.7015508491902095	2.0220773600405515
6	0.45718690981467225	1.581297721829145
7	0.3646161785208967	1.4713045658197141
8	0.27517821646473883	1.2690322125775328
9	0.22560121821410817	1.1704491688589471
>10	1.56220214849701	18.0405181157997
>50	0.15796700261566302	6.201266252316386
>100	0.05152023535418514	4.417834604196529
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.4498815482271435E-4	0.0
10	0.0	0.0	0.0	2.4498815482271435E-4	0.0
11	0.0	0.0	0.0	2.4498815482271435E-4	0.0
12	0.0	0.0	0.0	2.4498815482271435E-4	0.0
13	0.0	0.0	0.0	2.4498815482271435E-4	0.0
14	0.0	0.0	0.0	2.4498815482271435E-4	0.0
15	0.0	0.0	0.0	2.4498815482271435E-4	0.0
16	0.0	0.0	0.0	4.899763096454287E-4	0.0
17	0.0	0.0	0.0	4.899763096454287E-4	0.0
18	0.0	0.0	0.0	4.899763096454287E-4	0.0
19	0.0	0.0	0.0	4.899763096454287E-4	0.0
20	0.0	0.0	0.0	7.349644644681429E-4	0.0
21	0.0	0.0	0.0	7.349644644681429E-4	0.0
22	0.0	0.0	0.0	0.0014699289289362859	0.0
23	0.0	0.0	0.0	0.0029398578578725717	0.0
24	0.0	0.0	0.0	0.004164798631986143	0.0
25	0.0	0.0	0.0	0.004409786786808857	0.0
26	0.0	0.0	0.0	0.0073496446446814296	0.0
27	0.0	0.0	0.0	0.02033401685028529	0.0
28	0.0	0.0	0.0	0.07202651751787802	0.0
29	0.0	0.0	0.0	0.16169218218299145	0.0
30	0.0	0.0	0.0	0.2807564254268306	0.0
31	0.0	0.0	0.0	0.7254099264300571	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAATG	25	0.005492242	29.6	15
AACATAA	25	0.005492242	29.6	31
TTATTCT	40	5.930889E-5	27.750002	4
CGGCTAT	35	8.8595593E-4	26.42857	28
GTAGACT	80	2.7148417E-8	23.125002	16
GTACTAT	40	0.0019290653	23.125002	1
CTTATAC	315	0.0	22.90476	37
TTTATTC	65	2.6758116E-6	22.76923	3
TATACAC	90	3.810783E-9	22.61111	37
GGTATCA	165	0.0	22.424242	1
TTGTAGA	85	5.160109E-8	21.764706	2
CTAGACA	135	1.8189894E-12	20.555555	4
AGGACTA	45	0.0038216452	20.555555	5
TAAAACA	45	0.0038216452	20.555555	4
GGCCCTT	45	0.0038216452	20.555555	33
ACATGTA	100	1.2836608E-8	20.35	8
TCTAGAC	140	3.6379788E-12	19.821428	3
GCGTTAT	85	1.2412856E-6	19.588234	1
TGACCCG	85	1.2412856E-6	19.588234	13
TAGACTC	95	1.6695049E-7	19.473684	17
>>END_MODULE