FastQCFastQC Report
Fri 10 Feb 2017
ERR1632301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39155
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT1250.31924403013663644TruSeq Adapter, Index 12 (95% over 22bp)
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC900.22985570169837827No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC630.16089899118886475No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT590.1506831822244924No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT570.14557527774230622No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG560.14302132550121313No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT540.13791342101902695No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG490.1251436598135615No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG490.1251436598135615No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC480.1225897075724684No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA460.11748180309028222No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT450.11492785084918913No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT420.10726599412590986No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC410.10471204188481677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA506.9558155E-933.31
CCGTCTT250.005432539629.59999837
GCCGTCT250.005432539629.59999836
GGAACGA250.005432539629.59999819
GTATCAA604.1030944E-827.7499982
CAACGCA701.8262472E-723.7857136
AGGCTTC400.001898246923.12525
TCAACGC753.553705E-722.25
ATCAACG753.553705E-722.24
AACGCAG806.614009E-720.81257
TATCAAC806.614009E-720.81253
CGGGTGT656.6871224E-519.92307714
GCGGGTG656.6871224E-519.92307713
AGTACAT758.896903E-619.73333414
ACATGGG801.5527106E-518.517
ACGCAGA902.0463685E-618.58
GTACATG801.5527106E-518.515
CAGAGTA902.0463685E-618.511
AGAGTAC902.0463685E-618.512
GAGTACA801.5527106E-518.513