Basic Statistics
Measure | Value |
---|---|
Filename | ERR1632301.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 39155 |
Sequences flagged as poor quality | 0 |
Sequence length | 43 |
%GC | 54 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT | 125 | 0.31924403013663644 | TruSeq Adapter, Index 12 (95% over 22bp) |
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC | 90 | 0.22985570169837827 | No Hit |
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC | 63 | 0.16089899118886475 | No Hit |
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT | 59 | 0.1506831822244924 | No Hit |
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT | 57 | 0.14557527774230622 | No Hit |
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG | 56 | 0.14302132550121313 | No Hit |
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT | 54 | 0.13791342101902695 | No Hit |
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG | 49 | 0.1251436598135615 | No Hit |
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG | 49 | 0.1251436598135615 | No Hit |
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC | 48 | 0.1225897075724684 | No Hit |
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA | 46 | 0.11748180309028222 | No Hit |
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT | 45 | 0.11492785084918913 | No Hit |
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT | 42 | 0.10726599412590986 | No Hit |
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC | 41 | 0.10471204188481677 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 50 | 6.9558155E-9 | 33.3 | 1 |
CCGTCTT | 25 | 0.0054325396 | 29.599998 | 37 |
GCCGTCT | 25 | 0.0054325396 | 29.599998 | 36 |
GGAACGA | 25 | 0.0054325396 | 29.599998 | 19 |
GTATCAA | 60 | 4.1030944E-8 | 27.749998 | 2 |
CAACGCA | 70 | 1.8262472E-7 | 23.785713 | 6 |
AGGCTTC | 40 | 0.0018982469 | 23.125 | 25 |
TCAACGC | 75 | 3.553705E-7 | 22.2 | 5 |
ATCAACG | 75 | 3.553705E-7 | 22.2 | 4 |
AACGCAG | 80 | 6.614009E-7 | 20.8125 | 7 |
TATCAAC | 80 | 6.614009E-7 | 20.8125 | 3 |
CGGGTGT | 65 | 6.6871224E-5 | 19.923077 | 14 |
GCGGGTG | 65 | 6.6871224E-5 | 19.923077 | 13 |
AGTACAT | 75 | 8.896903E-6 | 19.733334 | 14 |
ACATGGG | 80 | 1.5527106E-5 | 18.5 | 17 |
ACGCAGA | 90 | 2.0463685E-6 | 18.5 | 8 |
GTACATG | 80 | 1.5527106E-5 | 18.5 | 15 |
CAGAGTA | 90 | 2.0463685E-6 | 18.5 | 11 |
AGAGTAC | 90 | 2.0463685E-6 | 18.5 | 12 |
GAGTACA | 80 | 1.5527106E-5 | 18.5 | 13 |