##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632301.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39155
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.258664282977907	31.0	31.0	34.0	30.0	34.0
2	31.49771421274422	31.0	31.0	34.0	30.0	34.0
3	31.529638615757886	31.0	31.0	34.0	30.0	34.0
4	35.370271995913676	37.0	35.0	37.0	33.0	37.0
5	34.47444770782786	35.0	35.0	37.0	30.0	37.0
6	34.39675648065381	35.0	35.0	37.0	30.0	37.0
7	34.36840761077768	35.0	35.0	37.0	30.0	37.0
8	34.32151704763121	35.0	35.0	37.0	30.0	37.0
9	35.93142638232665	37.0	35.0	39.0	31.0	39.0
10	35.62691865662112	37.0	35.0	39.0	30.0	39.0
11	35.80643595964755	37.0	35.0	39.0	30.0	39.0
12	35.74677563529562	37.0	35.0	39.0	30.0	39.0
13	35.85120674243392	37.0	35.0	39.0	30.0	39.0
14	36.81353594687779	38.0	36.0	40.0	31.0	41.0
15	36.73124760566977	38.0	36.0	40.0	31.0	41.0
16	36.683182224492406	38.0	36.0	40.0	31.0	41.0
17	36.59450900268165	38.0	36.0	40.0	31.0	41.0
18	36.5593410803218	38.0	36.0	40.0	31.0	41.0
19	36.60472481164602	38.0	35.0	40.0	31.0	41.0
20	36.54095262418593	38.0	35.0	40.0	30.0	41.0
21	36.49993615119397	38.0	35.0	40.0	30.0	41.0
22	36.43856467884051	38.0	35.0	40.0	30.0	41.0
23	36.361511939726725	38.0	35.0	40.0	30.0	41.0
24	36.25404162942153	38.0	35.0	40.0	30.0	41.0
25	36.145064487294086	38.0	35.0	40.0	30.0	41.0
26	35.931094368535305	38.0	34.0	40.0	29.0	41.0
27	35.83657259609245	38.0	34.0	40.0	29.0	41.0
28	35.70483973949687	38.0	34.0	40.0	29.0	41.0
29	35.56112884689056	38.0	34.0	40.0	28.0	41.0
30	35.424262546290386	38.0	34.0	40.0	27.0	41.0
31	35.380538883922874	38.0	34.0	40.0	27.0	41.0
32	35.207355382454345	38.0	34.0	40.0	27.0	41.0
33	35.10721491508109	38.0	33.0	40.0	27.0	40.0
34	35.0315413101775	38.0	33.0	40.0	26.0	41.0
35	34.89827608223726	38.0	33.0	40.0	26.0	40.0
36	34.86106499808454	37.0	33.0	39.0	26.0	40.0
37	34.74174434938067	37.0	33.0	39.0	26.0	40.0
38	34.71791597497127	37.0	33.0	39.0	26.0	40.0
39	34.60623164346827	37.0	33.0	39.0	26.0	40.0
40	34.466555995402885	37.0	33.0	39.0	25.0	40.0
41	34.39920827480526	37.0	33.0	39.0	25.0	40.0
42	34.31796705401609	37.0	32.0	39.0	25.0	40.0
43	33.3191674115694	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	3.0
17	0.0
18	4.0
19	8.0
20	26.0
21	37.0
22	87.0
23	117.0
24	158.0
25	244.0
26	329.0
27	479.0
28	718.0
29	911.0
30	1295.0
31	1638.0
32	2182.0
33	2784.0
34	3628.0
35	4703.0
36	5783.0
37	6531.0
38	5743.0
39	1740.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.699527518835396	18.516153747924914	12.470948793257566	26.313369939982124
2	22.339420252841272	19.19805899629677	31.43659813561486	27.025922615247094
3	22.607585238156048	20.937300472481166	27.80487804878049	28.6502362405823
4	17.606946750095773	15.333929255522921	30.476312092963866	36.58281190141744
5	17.75252202783808	33.1605158983527	31.01264206359341	18.074320010215807
6	38.053888392287064	31.418720469927212	13.678968203294598	16.848422934491126
7	31.60260503128591	27.78955433533393	18.830289873579364	21.777550759800793
8	29.47771676669646	30.054909973183502	18.549355127059126	21.91801813306091
9	27.77167666964628	13.244796322308773	17.75252202783808	41.23100498020687
10	19.844208913293322	24.004597114033967	30.593793896054144	25.55740007661857
11	38.17137019537735	19.55305835780871	18.56978674498787	23.705784701826076
12	24.313625335206233	23.524454092708467	25.690205593155408	26.471714978929896
13	32.69058868599157	17.313242242370066	22.518196909717787	27.477972161920572
14	24.92401992082748	19.499425360745754	21.808198186693907	33.768356531732856
15	29.15081087983655	24.22679095900907	19.259353850083002	27.363044311071384
16	27.672072532243646	23.054526880347336	21.09053760694675	28.182862980462264
17	25.976248244157834	24.6584088877538	22.42114672455625	26.944196143532118
18	26.566211211850337	21.557910867066784	23.841144170604007	28.034733750478868
19	27.55969863363555	22.880858127953008	22.72762099348742	26.83182224492402
20	26.750095773209043	22.357297918528925	23.465713191163324	27.42689311709871
21	29.776529178904354	21.688162431362535	22.02273017494573	26.512578214787386
22	29.06397650363938	22.375175584216574	22.257693781126292	26.30315413101775
23	28.216064359596476	22.490103435065762	21.61920572085302	27.674626484484737
24	28.03217979823777	22.37262163197548	22.08913293321415	27.506065636572597
25	27.401353594687777	22.548844336610905	22.62801685608479	27.421785212616523
26	28.157323458051337	22.878304175711914	22.64589452177244	26.31847784446431
27	28.338654067168946	22.441578342485	22.255139828885202	26.964627761460864
28	26.637721874600945	21.843953518069213	23.81560464819308	27.70271995913676
29	26.862469671817134	21.61920572085302	24.096539394713318	27.421785212616523
30	25.8408887753799	22.556506193334187	24.349380666581535	27.25322436470438
31	27.217469033329078	23.051972928106245	22.559060145575277	27.1714978929894
32	26.808836674754183	22.367513727493296	23.299706295492275	27.523943302260246
33	27.255778316945474	22.832333035372237	22.538628527646534	27.373260120035752
34	27.490741923126038	22.725067041246326	22.612693142638232	27.1714978929894
35	27.010598901800538	22.725067041246326	24.083769633507853	26.18056442344528
36	27.237900651257824	23.069850593793895	22.078917124249777	27.613331630698507
37	26.30315413101775	22.142765930277104	23.542331758396116	28.011748180309027
38	26.97995147490742	22.071255267526496	24.14506448729409	26.803728770271995
39	25.766824160388204	21.76733495083642	25.143659813561488	27.32218107521389
40	25.483335461626865	21.029242753160517	26.116715617417952	27.370706167794662
41	24.640531222066148	21.453198825181968	26.49980845358192	27.406461499169964
42	24.56902055931554	21.690716383603625	26.282722513089006	27.457540543991826
43	24.173157961946114	20.120035755331376	27.523943302260246	28.182862980462264
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.5
4	3.0
5	3.0
6	5.0
7	7.0
8	8.0
9	9.0
10	11.5
11	14.0
12	14.0
13	24.5
14	35.0
15	43.0
16	51.0
17	52.5
18	54.0
19	54.0
20	64.0
21	74.0
22	77.0
23	80.0
24	91.0
25	102.0
26	102.0
27	140.5
28	179.0
29	233.0
30	287.0
31	382.0
32	477.0
33	477.0
34	613.5
35	750.0
36	894.5
37	1039.0
38	1181.0
39	1323.0
40	1323.0
41	1487.5
42	1652.0
43	1810.0
44	1968.0
45	2155.5
46	2343.0
47	2343.0
48	2430.0
49	2517.0
50	2736.0
51	2955.0
52	3149.0
53	3343.0
54	3343.0
55	3407.0
56	3471.0
57	3459.5
58	3448.0
59	3131.5
60	2815.0
61	2815.0
62	2789.5
63	2764.0
64	2355.5
65	1947.0
66	1719.5
67	1492.0
68	1492.0
69	1249.5
70	1007.0
71	945.5
72	884.0
73	900.5
74	917.0
75	917.0
76	817.0
77	717.0
78	480.0
79	243.0
80	173.0
81	103.0
82	103.0
83	69.0
84	35.0
85	28.5
86	22.0
87	20.0
88	18.0
89	18.0
90	12.5
91	7.0
92	4.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	39155.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.43608734516664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.13102210786471	67.99131656238029
2	5.135931204929411	7.74869109947644
3	1.7638893591089142	3.991827352828502
4	0.7549852727088059	2.2781253990550376
5	0.5484646375732132	2.0687013152854044
6	0.3521007549852727	1.593666198442089
7	0.26746115042150526	1.4123355893244796
8	0.16927920912753494	1.0215808964372366
9	0.11172427802417306	0.7585238156046481
>10	0.7414429359786031	9.84803984165496
>50	0.020313505095304194	0.9679478993742817
>100	0.003385584182550699	0.31924403013663644
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	125	0.31924403013663644	TruSeq Adapter, Index 12 (95% over 22bp)
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	90	0.22985570169837827	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	63	0.16089899118886475	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	59	0.1506831822244924	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	57	0.14557527774230622	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	56	0.14302132550121313	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	54	0.13791342101902695	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	49	0.1251436598135615	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG	49	0.1251436598135615	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	48	0.1225897075724684	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	46	0.11748180309028222	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	45	0.11492785084918913	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	42	0.10726599412590986	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	41	0.10471204188481677	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.012769761205465458	0.0
27	0.0	0.0	0.0	0.0306474268931171	0.0
28	0.0	0.0	0.0	0.0919422806793513	0.0
29	0.0	0.0	0.0	0.1481292299833993	0.0
30	0.0	0.0	0.0	0.22730174945728515	0.0
31	0.0	0.0	0.0	0.3882007406461499	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	50	6.9558155E-9	33.3	1
CCGTCTT	25	0.0054325396	29.599998	37
GCCGTCT	25	0.0054325396	29.599998	36
GGAACGA	25	0.0054325396	29.599998	19
GTATCAA	60	4.1030944E-8	27.749998	2
CAACGCA	70	1.8262472E-7	23.785713	6
AGGCTTC	40	0.0018982469	23.125	25
TCAACGC	75	3.553705E-7	22.2	5
ATCAACG	75	3.553705E-7	22.2	4
AACGCAG	80	6.614009E-7	20.8125	7
TATCAAC	80	6.614009E-7	20.8125	3
CGGGTGT	65	6.6871224E-5	19.923077	14
GCGGGTG	65	6.6871224E-5	19.923077	13
AGTACAT	75	8.896903E-6	19.733334	14
ACATGGG	80	1.5527106E-5	18.5	17
ACGCAGA	90	2.0463685E-6	18.5	8
GTACATG	80	1.5527106E-5	18.5	15
CAGAGTA	90	2.0463685E-6	18.5	11
AGAGTAC	90	2.0463685E-6	18.5	12
GAGTACA	80	1.5527106E-5	18.5	13
>>END_MODULE